| Z702668 Sigma |
Apoptosis Methods and Protocols |
General description
A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in detail, these reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. This book constitutes a key technical reference to the significant methodologies used in the field and powerful tools to illuminate the phenomenon of programmed cell death.
Table of Contents
1. Measurement of Apoptosis by DNA Fragmentation
2. Measurement of Caspase Activity in Cells Undergoing Apoptosis
3. Flow Cytometry Based Methods for Apoptosis Detection in Lymphoid Cells
4. Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays
5. Detecting and Quantifying Apoptosis in Tissue Sections
6. Immunoassay for Single Stranded DNA in Apoptotic Cells
7. Methods to Measure Membrane Potential and Permability Transition in Mitochondria During Apoptosis
8. Measurement of Changes in Intracellular Calcium During Apoptosis
9. Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
10. Assays to Measure Stress Activated MAPK Activity
11. Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability
12. Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
13. Measuring Programmed Cell Death in Plants
14. Detection of Apoptosis in Drosophila
15. Measurement of Apoptotic Cell Clearance In Vitro
16. Yeast Two Hybrid Screening for Proteins Interacting WIth the Anti-Apoptotic Protein A20
17. The Yeast Three Hybrid System As a Tool to Study Caspases
18. Cloning of Apoptosis Related Genes by Representational Difference Analysis of cDNA
19. Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis
2. Measurement of Caspase Activity in Cells Undergoing Apoptosis
3. Flow Cytometry Based Methods for Apoptosis Detection in Lymphoid Cells
4. Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays
5. Detecting and Quantifying Apoptosis in Tissue Sections
6. Immunoassay for Single Stranded DNA in Apoptotic Cells
7. Methods to Measure Membrane Potential and Permability Transition in Mitochondria During Apoptosis
8. Measurement of Changes in Intracellular Calcium During Apoptosis
9. Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2
10. Assays to Measure Stress Activated MAPK Activity
11. Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability
12. Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons
13. Measuring Programmed Cell Death in Plants
14. Detection of Apoptosis in Drosophila
15. Measurement of Apoptotic Cell Clearance In Vitro
16. Yeast Two Hybrid Screening for Proteins Interacting WIth the Anti-Apoptotic Protein A20
17. The Yeast Three Hybrid System As a Tool to Study Caspases
18. Cloning of Apoptosis Related Genes by Representational Difference Analysis of cDNA
19. Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis
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