Plant Cell Culture 

1-85996320-X

978-1-85996320-3

D. Evans, et al., BIOS Scientific, 2003, 256 pp., soft cover

Ch 1. AN INTRODUCTION TO PLANT CELL AND TISSUE CULTURE
Understanding function and development
Horticultural/agricultural developments
Role in development of molecular biology
Ch 2. BASIC PLANT BIOLOGY FOR CELL CULTURE
Meristem
Organs
Anthers and pollen
Embryos and Embryogenesis
Callus and protoplasts
Nutrients, media and requirements for growth
Ch 3. TISSUE CULTURE IN AGRICULTURE, HORTICULTURE AND FORESTRY
Micropropagation
Plant breeding
Cloning
Ch 4. TISSUE CULTURE IN GENETIC ENGINEERING AND BIOTECHNOLOGY
Start points - cultures, tissues and species
Selectable markers
Transient expression using suspension culture and protoplasts
Stable transformation systems based on Agrobacterium tumifaciens
Stable transformation using other methods
Growing transformed plants
Embryo rescue
Laboratory uses and agricultural/industrial applications
Ch 5. BASIC STERILE TECHNIQUE AND MEDIA PREPARATION
The laboratory
The equipment
Working in sterile conditions
Culture growth conditions including lighting, temperature etc
Contamination - how to avoid it, what to do when it happens
New methods to inhibit bacteria and fungi
Health and safety
Ch 6. CALLUS CULTURE
Introduction
Techniques
Monitoring growth
Organogenesis
Ch 7. SUSPENSION CULTURE
Introduction
Techniques
Monitoring growth
Applications including whole plants from suspension culture
Ch 8. PROTOPLASTS
Introduction
Techniques
Applications including protoplast fusion, whole plants from photoplasts
Flow Cytometry
Ch 9. HAPLOID CULTURES
Introduction
Techniques
Applications
To include ferns, mosses and lower plants
Ch 10. ORGAN CULTURE
Root tip
Shoot tip and axillary bud culture
Leaf
Flowers
Fruit
Applications including virus-free plants
Ch 11. REGENERATING PLANTS/MICROPROPAGATION
Start points
Culture requirements
Acclimation
Ch 12. SOMACLONAL VARIATION AND ARTIFICIAL MUTAGENESIS
Introduction
Screening
Applications
Ch 13. BACTERIAL CULTURE FOR PLANT SCIENCE
Agrobacterium tunifacens
Escherichia coli product of competent cells etc
Ch 14. CELL CULTURE IN PLANT PATHOLOGY
Introduction
Bacteria
Fungi
Viruses
Other pathogens
Ch 15. SCALING UP
Secondary products
Continuous and fed batch cultures
Production of industrial and medicinal compounds
Ch 16. PROSPECTS AND PROBLEMS-CHALLENGES FOR PLANT CELL CULTURE
Clonal propagation and disease
Intractable species
Genetic conservation
Public perception
High value products and green agriculture