B Cell Protocols 

1-58829347-5

978-1-58829347-3

H. Gu and K. Rajewsky, ed., Humana Press, 2004, 304 pp., hard cover

1. Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen
2. Characterization of B Cell Matruation in the Peripheral Immune System
3. Identification of B Cell Subsets: An exposition of 11- Color (Hi-D) FACS Methods
4. Analysis of B Cell Life Span and Homeostasis
5. Differentiation of B Lymphocytes From Hematopoietic Stem Cells
6. Analysis of lymphocyte Development and Function Usgin the RAG Deficient Blastocyst Complementation System
7. Conditional Gene Mutagenesis in B Lineage Cells
8. Analysis of the Germinal Center Reaction and In Vivo Long Lived Plasma Cells
9. T Cell Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences
10. Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
11. Immunoglobulin Class Switching: In Vitro Induction and Analysis
12. In Vitro and In Vivo Assays of B Lymphocyte Migration
13. Analysis of Antigen Specific B Cell Memory Directly Ex Vivo
14. B Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
15. Isolation of Lipid Rafts From B Lymphocytes
16. Molecular Single Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells
17. Analysis of B Cell Immune Tolerance Induction Using Transgenic Mice
18. Analysis of B Cell Signaling Using DT40 B Cell Line
19. B Cell Development and Pre-B-1 Cell Plasticity In Vitro