| Z703702 Sigma |
Amyloid Precursor Protein: A Practical Approach |
General description
Amyloid precursor protein (APP) is an extensively studied transmembrane protein, and its derivative, amyloid β-protein, has been implicated as the primary cause of Alzheimer's disease. In the search for an effective treatment, APP is a unique model protein that illustrates the wide array of techniques available for characterization. This book presents a variety of biological techniques to elucidate the structure and function of this transmembrane protein. Each technique is presented with detailed protocols to achieve reproducible results and provide a framework for studying other membrane proteins. These protocols also form the core of the authors' approaches to elucidating the function of APP. This book contrasts methods to explain how the development of biological techniques affects the way molecular targets are examined.
Table of Contents
Antibodies to APP & Aβ Proteins
Biochemical Characterization of Amyloid Precursor Protein
Assays for Analysis of APP Secretion and Recycling
Strategies for Crystallizing the N-Terminal Growth Factor Domain of Amyloid Precursor Protein
Analysis of Amyloid Precursor Protein Processing Protease β-Secretase: Tools For Memapsin 2 (β-Secretase) Inhibition Studies
Assays for Amyloid Precursor Protein γ-Secretase Activity
Cell-Free Reconstitution of β-Amyloid Production and Trafficking
Studying Amyloid β-Protein Assembly
Intracellular Accumulation of Amyloid β and Mitochondrial Dysfunction in Down Syndrome
Linking Alzheimer's Disease, β-Amyloid, and Lipids: A Technical Approach
Regulation of Amyloid Precursor Protein Processing by Lithium
Immunocytochemical Analysis of Amyloid Precursor Protein and its Derivatives
Pathological Detection of Aβ and APP in the Brain
Creating APP Transgenic Lines in Mice
Generation of Amyloid Precursor Protein Knockout Mice
Biochemical Characterization of Amyloid Precursor Protein
Assays for Analysis of APP Secretion and Recycling
Strategies for Crystallizing the N-Terminal Growth Factor Domain of Amyloid Precursor Protein
Analysis of Amyloid Precursor Protein Processing Protease β-Secretase: Tools For Memapsin 2 (β-Secretase) Inhibition Studies
Assays for Amyloid Precursor Protein γ-Secretase Activity
Cell-Free Reconstitution of β-Amyloid Production and Trafficking
Studying Amyloid β-Protein Assembly
Intracellular Accumulation of Amyloid β and Mitochondrial Dysfunction in Down Syndrome
Linking Alzheimer's Disease, β-Amyloid, and Lipids: A Technical Approach
Regulation of Amyloid Precursor Protein Processing by Lithium
Immunocytochemical Analysis of Amyloid Precursor Protein and its Derivatives
Pathological Detection of Aβ and APP in the Brain
Creating APP Transgenic Lines in Mice
Generation of Amyloid Precursor Protein Knockout Mice
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