| Z703753 Sigma |
Cell Cycle Control: Mechanisms and Protocols |
General description
This collection of techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these reproducible methods enable fundamental research on well-defined cell cycle regulators and those more recently defined in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.
Table of Contents
PART I. Overviews of Cell Cycle Control in Different Organisms
1. Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts
2. The Plant Cell Cycle: An Overview
3. The C. elegans Cell Cycle: Oveview of Molecules and Mechanisms
4. Developmental Control of Growth and Cell Cycle Progression in Drosophila
5. The Xenopus Cell Cycle: An Overview
6. The Mammalian Cell Cycle: An Overview
PART II Cell Cycle Study Methods and Techniques
7. Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
8. Mapping Origins of DNA Replication in Eukaryotes
9. In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
10. Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs
11. The Cell Cycle and Virus Infection
12. Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies
PART III Detection and Measurement of Cell Cycle Molecules
13. E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
14. Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors
15. Measurement of Geminin Activity in Xenopus Egg Extracts
16. CDK-Activating Kinases: Detection and Activity Measurements
17. Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
18. Measurement of Wee Kinase Activity
19. CDC25 Dual Specificity Protein Phosphatases: Detection and Activity Measurements
20. Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
21. Polo-Like Kinase 1: Activity Measurement and RNAi Mediated Knockdown
22. The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
23. Purification of the Ndc80 Kinetochore Subcomplex from Xenopus Eggs
Index
1. Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts
2. The Plant Cell Cycle: An Overview
3. The C. elegans Cell Cycle: Oveview of Molecules and Mechanisms
4. Developmental Control of Growth and Cell Cycle Progression in Drosophila
5. The Xenopus Cell Cycle: An Overview
6. The Mammalian Cell Cycle: An Overview
PART II Cell Cycle Study Methods and Techniques
7. Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
8. Mapping Origins of DNA Replication in Eukaryotes
9. In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast
10. Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs
11. The Cell Cycle and Virus Infection
12. Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies
PART III Detection and Measurement of Cell Cycle Molecules
13. E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression
14. Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors
15. Measurement of Geminin Activity in Xenopus Egg Extracts
16. CDK-Activating Kinases: Detection and Activity Measurements
17. Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements
18. Measurement of Wee Kinase Activity
19. CDC25 Dual Specificity Protein Phosphatases: Detection and Activity Measurements
20. Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
21. Polo-Like Kinase 1: Activity Measurement and RNAi Mediated Knockdown
22. The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells
23. Purification of the Ndc80 Kinetochore Subcomplex from Xenopus Eggs
Index
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