Z703990
Sigma

Culture of Animal Cells: A Manual of Basic Techniques, 5th ed.

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General description

This fifth and fully revised edition of Culture of Animal Cells continues to provide the most comprehensive information on the techniques, equipment, principles, and practices of animal cell culture. It offers a complete background related to growth of animal cells in tissue culture, beginning with laboratory design, safety, and bioethics, then continuing to coverage of preparation through to contamination, molecular techniques, and troubleshooting.

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Identifiers

ISBN-10

0-471-45329-3

ISBN-13

978-0-471-45329-1

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publication info

R. Ian Freshney, John Wiley & Sons, 2005, 672 pp., hard cover

Table of Contents

1. Introduction
2. Training Programs
3. Biology of Cultured Cells
4. Laboratory Design and Layout
5. Equipment
6. Aseptic Technique
7. Safety, Bioethics and Validation
8. Culture Vessels and Substrates
9. Defined Media and Supplements
10. Serum Free Media
11. Preparation and Sterilization
12. Primary Culture
13. Subculture and Cell Lines
14. Cloning and Selection
15. Cell Separation
16. Characterization
17. Differentiation
18. Transformation and Immortalization
19. Contamination
20. Cryopreservation
21. Quantitation
22. Cytotoxicity
23. Culture of Specific Cell Types
24. Culture of Tumor Cells
25. Organotypic Culture
26. Scale Up
27. Specialized Techniques
28. Problem Solving
29. In Conclusion

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Z703990 (Sigma)