Z704083
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Analytical Techniques in DNA Sequencing

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General description

This text takes a look at the various DNA sequencing techniques that were developed and put to use during and after the Human Genome Project. Special attention is paid to the breakthrough Sanger Method, as well as to several other techniques that improved the accuracy, improved the detection limits, and in a variety of ways dramatically reduced the time needed to generate a DNA sequence. This volume describes the improvements in DNA separation based on advanced capillary electrophoresis and microchip sequencing. It also looks at the use of mass spectrometry and single molecule detection, and takes an in-depth look at the use of various fluorescence methods for the detection of sequencing fragments. One chapter is dedicated to chip-based microfabricated sequencing systems, which could well be the future of DNA sequencing.

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Identifiers

ISBN-10

0-82475342-9

ISBN-13

978-0-82475342-9

Properties

publication info

B. Nunnally, CRC Press, 2005, 256 pp., hard cover

Table of Contents

Introduction to DNA Sequencing: Sanger and Beyond,
Developments in the Detection of DNA Sequencing Fragments Using Fluorescence: Energy Transfer and Fluorescence Lifetimes,
Microscale Sample Preparation for DNA Sequencing and Genotyping,
Microfabricated DNA Sequencing Devices,
Analysis of Nucleic Acids by Mass Spectrometry,
Sequencing the Single DNA Molecule,
DNA Sequencing for Genome Analysis,
Sequence Reconstruction from Nucleic-Acid Micro-Array Data,
Ancient DNA,
Forensic DNA Sequencing,