| Z704199 Sigma |
DNA Methylation: Approaches, Methods and Applications |
General description
A guide to understanding the complex regulatory roles of DNA methylation, this volume describes the relation that this biological mechanism has to gene silencing in disease and explores its promising role in treating cancer. The book is a state-of-the-art reference for understanding the role DNA methylation plays in human disease, particularly cancer. This compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation status of genomic DNA. It includes concepts, experimental models, and clinical uses of demethylating agents. The book provides a balance between articles clarifying methodological details and more general review chapters.
Table of Contents
Impact of DNA Methylation in Human Health and Disease;
Uses of DNA Methylation in Cancer Diagnosis and Risk Assessment;
DNA Methylation in Imprinting, X-Chromosome Inactivation and Associated Diseases;
Studying Mammalian DNA Methylation: Bisulfite Modification;
Bisulfite PCR-Based Techniques for the Study of DNA Methylation;
Microarray Analysis of DNA Methylation Targets Identified by Methyl-CpG Binding Proteins;
Methylation Arbitrarily-Primed PCR and Derived Methodologies;
Discovering DNA Methylation Differences with Restriction Landmark Genome Scanning;
Quantitative Determination of 5-methylcytosine DNA Content: HPCE and HPLC;
Qualitative Determination of 5-methylcitosine DNA and other Components of the DNA Methylation Machinery: Immunofluorescence and Chromatin Immunoprecipitation;
Mouse Models for the Study of DNA Methylation;
DNA Demethylating Agents: Concepts;
DNA Demethylating Agents: Preclinical Evaluation as Anticancer Agents;
DNA Demethylating Agents: Clinical Uses;
Uses of DNA Methylation in Cancer Diagnosis and Risk Assessment;
DNA Methylation in Imprinting, X-Chromosome Inactivation and Associated Diseases;
Studying Mammalian DNA Methylation: Bisulfite Modification;
Bisulfite PCR-Based Techniques for the Study of DNA Methylation;
Microarray Analysis of DNA Methylation Targets Identified by Methyl-CpG Binding Proteins;
Methylation Arbitrarily-Primed PCR and Derived Methodologies;
Discovering DNA Methylation Differences with Restriction Landmark Genome Scanning;
Quantitative Determination of 5-methylcytosine DNA Content: HPCE and HPLC;
Qualitative Determination of 5-methylcitosine DNA and other Components of the DNA Methylation Machinery: Immunofluorescence and Chromatin Immunoprecipitation;
Mouse Models for the Study of DNA Methylation;
DNA Demethylating Agents: Concepts;
DNA Demethylating Agents: Preclinical Evaluation as Anticancer Agents;
DNA Demethylating Agents: Clinical Uses;
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