Z704393
Sigma

Chemical Genomics: Reviews and Protocols

1 of 1

General description

An up-to-date introduction for the uninitiated into the various “flavors” of chemical genomics with a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, emphasizing the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches to protein–small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports.

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Identifiers

ISBN-10

1-58829-399-8

ISBN-13

978-1-58829-399-2

Properties

publication info

E. Zanders, Humana Press, 2005, 296 pp., hard cover

Table of Contents

Part I. Reviews Introduction to Chemical Genomics
Chemistry for Chemical Genomics
Computer-Aided Design of Small Molecules for Chemical Genomics
Part II. Protocols Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe
The Role and Application of In Silico Docking in Chemical Genomics Research Aldo Jongejan, Chris de Graaf, Nico P. E. Vermeulen, Rob Leurs, and Iwan J. P. de Esch
Synthesis of Complex Carbohydrates and Glyconjugates: Enzymatic Synthesis of Globotetraose Using b-1,3-N-Acetylgalactosaminyltransferase LgtD From Haemophilus infuenzae Strain Rd Kang Ryu, Steven Lin, Jun Shao, Jing Song, Min Chen, Wei Wang, Hanfen Li, Wen Yi, and Peng George Wang
High-Throughput Cloning for Proteomics Research Sharon A. Doyle Screening for the Expression of Soluble Recombinant Protein in Escherichia coli Sharon A. Doyle
High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli Michael B. Murphy and Sharon A. Doyle
The Wheat Germ Cell-Free Expression System: Methods for High-Throughput Materialization of Genetic Information Tatsuya Sawasaki, Mudeppa D. Gouda, Takayasu Kawasaki, Takafumi Tsuboi, Yuzuru Tozawa, Kazuyuki Takai, and Yaeta Endo
Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System Yaeta Endo and Tatsuya Sawasaki
Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System Toshiyuki Kohno Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening Jon Hoyt and Randall W. King
Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis Jonathan M. Blackburn and Darren J. Hart
Peptide and Small-Molecule Microarrays Jan Marik and Kit S. Lam
Peptide Mass Fingerprinting: Protein Identification Using MALDI-TOF Mass Spectrometry Judith Webster and David Oxley A Practical Protocol for Carbohydrate Microarrays Ruobing Wang, Shaoyi Liu, Dhaval Shah, and Denong Wang
Development of a Yeast Two-Hybrid Screen for Selection of Human Ras–Raf Protein Interaction Inhibitors Vladimir Khazak, Erica A. Golemis, and Lutz Weber Index

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