Z705039
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Plant Cell Culture Protocols, 2nd ed.

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General description

A collection of the most frequently used techniques for plant cell and tissue culture. The readily reproducible methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

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Identifiers

ISBN-10

1-58829547-8

ISBN-13

978-1-58829547-7

Properties

publication info

V. Loyola-Vargas and F. Vasquez-Flota, Humana Press, 2006, 416 pp., hard cover

Table of Contents

PART I: INTRODUCTION
1. An Introduction to Plant Cell Culture: Back to the Future
2. History of Plant Tissue Culture
PART II: CELL CULTURE AND PLANT REGENERATION: THE FUNDAMENTALS
3. Pathogen and Biological Contamination Management: The Road Ahead
4. Growth Measurement: Estimation of Cell Division and Cell Expansion
5. Callus and Suspension Culture Induction, Maintenance, and Characterization
6. Measurement of Cell Viability in In Vitro Cultures
7. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation Vitrification
8. Somatic Embryogenesis in Picea Suspension Cultures
9. Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes
10. Direct Somatic embryogenesis in Coffea canephora
PART III: PLANT PROPAGATION IN VITRO
11. A New Temporary Immersion Bioreactor System for Micropropagation
12. Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro
13. Use of Statistics in Plant Biotechnology
14. An Efficient Methods for the Micropropagation of Agave Species
15. Micropropagation of Endangered Plant Species
16. Clonal Propagation of Softwoods
PART IV. APPLICATIONS FOR PLANT PROTOPLASTS
17. Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora
18. Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts
19. Production of Cybrids in Brassicaceae Species
20. Guard Cell Protoplasts: Isolation, Culture and Regeneration of Plants
21. Production of INterspecific Hybrid Plants in Primula
PART V. PROTOCOLS FOR GENOMIC MANIPULATION
22. Agrobacterium Mediated Transformation of Petunia Leaf Discs
23. Transformation of Wheat Via Particle Bombardment
24. Chloroplast Transformation
25. The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers
26. Transforamtion of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System
PART VI. ACCUMULATION OF METABOLITES IN PLANT CELLS
27. Cpsaicin Accumulation in Capsicumspp. Suspension Cultures
28. Isolation and PUrification of Ribosome-Inactivating Proteins
29. Catharnathus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids
30. Methods for Regeneration and Transformation in Eschscholzia californica: A Model Plant to Investigate Alkaloid Biosynthesis
Index

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