| Z705225 Sigma |
Calcium Signaling, 2nd ed. |
General description
In this edition, Putney focuses on those processes that generate calcium signals to compile the first comprehensive exploration of calcium signaling research from a methodological standpoint. This edition deals with methods for studying calcium from a variety of perspectives. Several chapters discuss calcium indicators and other tools, and look at microscopic and electrophysiological techniques, as well as other special methodological aspects of calcium signaling research. Other chapters examine the study of different systems, ranging from those found in yeast to those found in mammals, and several more are devoted to the cellular and molecular basis for calcium signaling, including explorations of receptors, calcium pumps, apoptosis, and drug delivery.
Table of Contents
Fluorescent Calcium Indicators Based on BAPTA;
Fluorescent Indicators-Facts and Artifacts;
Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling: The Highs and Lows for Calcium Researchers;
Genetically Encoded Fluorescent Calcium Indicator Proteins;
Targeted Aequorins;
Electrophysiological Recordings of Ca2o Currents;
Molecular Biology of Ca2o Channels: Lessons from the TRP Superfamily;
Genetic and Molecular Characterization of Ca2o and IP3 Signaling in the Nematode Caenorhabditis elegans;
Calcium Signaling Networks in Yeast;
Nuclear Patch Clamp Electrophysiology of Inositol Trisphosphate Receptor Ca2o Release Channels;
Ryanodine Receptors;
Methods in Cyclic ADP-Ribose and NAADP Research;
Methods for Studying Calcium Pumps;
Measuring Single Cell and Subcellular Ca2o Signals;
Subcellular Compartmentalization of Calcium Signaling;
Apoptosis;
Calcium Entry Channels and Drug Discovery;
Index
Fluorescent Indicators-Facts and Artifacts;
Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling: The Highs and Lows for Calcium Researchers;
Genetically Encoded Fluorescent Calcium Indicator Proteins;
Targeted Aequorins;
Electrophysiological Recordings of Ca2o Currents;
Molecular Biology of Ca2o Channels: Lessons from the TRP Superfamily;
Genetic and Molecular Characterization of Ca2o and IP3 Signaling in the Nematode Caenorhabditis elegans;
Calcium Signaling Networks in Yeast;
Nuclear Patch Clamp Electrophysiology of Inositol Trisphosphate Receptor Ca2o Release Channels;
Ryanodine Receptors;
Methods in Cyclic ADP-Ribose and NAADP Research;
Methods for Studying Calcium Pumps;
Measuring Single Cell and Subcellular Ca2o Signals;
Subcellular Compartmentalization of Calcium Signaling;
Apoptosis;
Calcium Entry Channels and Drug Discovery;
Index
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