Handbook of Assay Development in Drug Discovery 

1-57444471-9

978-1-57444471-1

L. Minor, CRC Press, 2006, 488 pp., hard cover

Protein Kinases in Drug Discovery: Rationale, Success, and Challenge;
An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development;
Time-Resolved Fluorescence Based Assays for Kinases;
Development of High-throughput Screening Assays in Scintillating Microplates (FlashPlates) to Identify Inhibitors of Kinase Activity;
Development of High-Throughput Screening Assays for Kinase Drug Targets using AlphaScreen™ Technology;
Homogeneous High-Throughput Screening (HTS) Assays for Serine/Threonine Kinases Using ß-Galactosidase Enzyme Fragment Complementation;
IMAP Assays for Assaying Protein Kinases;
A Homogenous, Luminescent, High-Throughput, Versatile Assay for a Wide Range of Kinases;
Proteases as Drug Targets;
A Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays;
Scintillation Proximity Assay (SPA) Receptor Binding Assays;
Radioligand Binding Filtration Assay: Full Automation;
Nuclear Receptors As Drug Targets;
Nuclear Receptor Scintillation Proximity Assays; D
Homogenous Assay Development for Nuclear Receptor Using the AlphaScreen Technology;
Development of Nuclear Receptor Homogenous Assay Using the Lance Technology;
The Emerging Role of Cell-based Assays in Discovery;
The Preparation of Cells for High-Content Screening;
The Evolution of cAMP Assays;
A Homogenous Fluorescent Polariaiton assay for Inositol 1,4,5-Trisphoshpate (Ins P3);
Scintillation Proximity Assay of Inositol Phosphates;
Measuring Calcium Mobilization with Gq-Coupled GPCRs Using the Fluorometric Imaging Plate Reader (FLIPR);
Development of FLIPR-Based HTS Assay for Gi-Coupled GPCRs;
Aurora Assays;
Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters;
Reporter Gene Assays for Drug Discovery;
mRNA Detection from Cells Using Quantigene® Branched DNA Technology;
Homogeneous Miltiwell Assays for Measuring Cell Viability, Cytotoxicity and Apoptosis;
Development of Image-Bases Assays for Drug Discovery;
GPCR Internalization Measured by Image Analysis;
TRANSFLUOR®, a Universal Cell-Based Assay for Screening G-Protein Coupled Receptors;