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Ion Channels: Methods and Protocols

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General description

It is now clear that ion channels play essential roles in cell biology and physiology and that their dysfunction is the root cause of many human diseases. In this book internationally recognized biomedical scientists describe recent technological braekthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterogenous expression of ion channels in cells for determining channel structure-function, for studying channel regulation and physiological function and for genetic screening and investigating channelopathies.

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Identifiers

ISBN-10

1-58829576-1

ISBN-13

978-1-58829576-7

Properties

publication info

J. Stockard and M. Shapiro, Humana Press, 2006, 400 pp., hard cover

Table of Contents

SECTION 1. Methods for Exogenous Expression Ion Channels in Cells
1. Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System
2. Overexpression of Proteins in Neurons Using Replication-Deficient Virus
3. Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System
SECTION 2. Methods for Studying Channel Structure-Function
4. Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels
5. Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System
6. Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis
SECTION 3. Methods for Studying Channel Regulation and Physiological Function
7. Probing the Effects of Phosphoinositides on Ion Channels
8. Epithelial Sodium Channel in Planar Lipid Bilayers
9. A Simple in-vivo Method for Assessing Changes of Membrane Bound Ion Channel Density in Xenopus Oocytes
10. Preparation of Cortical Brain Slices for Electrophysiological Recording
11. Juxtacellular Labeling and Chemical Phenotyping of Extracellulary Recorded Neurons In Vivo
12. Carbon Fiber Amperometry in the Study of Ion Channels and Secretion
SECTION 4. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology
13. Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Update-Deficient Escherichia coli
14. KCNQ1 K Channel-Mediated Cardiac Channelopathies
15. Tissue-Specific Transgenic and Knockout Mice

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