| Z705705 Sigma |
Cell-Cell Interactions: Methods and Protocols |
General description
A versatile collection of readily reproducible cell–cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell–cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
Table of Contents
1. Blood Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood Brain Barrier and Transport Measurements
2. Interactions of Intestinal Epithelial Cells with Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences
3. The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
4. Cell-Cell Interactions on Solid Matrices
5. Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function
6. Nucleotide Metabolism and Cell-Cell Interactions
7. Analysis of Mammalian Sperm-Egg Membrane Interactions during In Vitro Fertilization
8. Collagen Gel Contraction Assay
9. Methods to Assess Tissue Permeability
10. Bacterial-Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy
11. A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum
12. Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
13. Expression Cloning of Signaling Proteins Regulated by Cell Adhesion
14. Assays for the Calcium Sensitivity of Desmosomes
15. Tight Junctions and Cell-Cell Interactions
16. Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Section
17. In Vitro Neutrophil Transepithelial Migration
18. The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions
19. Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators
20. Isolation and Analysis of Lipid Rafts in Cell-Cell Interactions
Index
2. Interactions of Intestinal Epithelial Cells with Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences
3. The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
4. Cell-Cell Interactions on Solid Matrices
5. Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function
6. Nucleotide Metabolism and Cell-Cell Interactions
7. Analysis of Mammalian Sperm-Egg Membrane Interactions during In Vitro Fertilization
8. Collagen Gel Contraction Assay
9. Methods to Assess Tissue Permeability
10. Bacterial-Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy
11. A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum
12. Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
13. Expression Cloning of Signaling Proteins Regulated by Cell Adhesion
14. Assays for the Calcium Sensitivity of Desmosomes
15. Tight Junctions and Cell-Cell Interactions
16. Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Section
17. In Vitro Neutrophil Transepithelial Migration
18. The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions
19. Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators
20. Isolation and Analysis of Lipid Rafts in Cell-Cell Interactions
Index
Related Information
Links
Page Options| |
|
© 2008 Sigma-Aldrich Co. Reproduction forbidden without permission. |