| Z719242 Sigma |
Cell Imaging: Methods Express Serieshard cover |
General description
Cell Imaging: Methods Express covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but many of the techniques and approaches are equally relevant to the imaging of fixed specimens
The topics in this comprehensive research guide include:
Confocal versus widefield imaging; Transmitted light imaging; Photobleaching and photoactivation; Deconvolution and 3D reconstruction; Spectral imaging; Quantification and image analysis; Imaging calcium signals and activation of calcium binding proteins; Fluorescence Resonance Energy Transfer; Total Internal Reflection Fluorescence Microscopy; Correlative light-electron microscopy; Fluorescence Lifetime imaging; High Content and High Throughput imaging
The topics in this comprehensive research guide include:
Confocal versus widefield imaging; Transmitted light imaging; Photobleaching and photoactivation; Deconvolution and 3D reconstruction; Spectral imaging; Quantification and image analysis; Imaging calcium signals and activation of calcium binding proteins; Fluorescence Resonance Energy Transfer; Total Internal Reflection Fluorescence Microscopy; Correlative light-electron microscopy; Fluorescence Lifetime imaging; High Content and High Throughput imaging
Table of Contents
1. An introduction to cell imaging
2. Confocal or wide-field? A guide to selecting appropriate methods for cell imaging
3. Image quantification and analysis
4. Transmitted light imaging
5. Spectral imaging techniques for fluorescence microscopy
6. Measurement of protein motion by photobleaching
7. Imaging calcium and calcium-binding proteins
8. Total internal reflection fluorescence microscopy
9. An overview of three-dimensional and four-dimensional microscopy by computational deconvolution
10. Correlative microscopy using Tokuyasu cryosections: Applications for immunolabeling and in situ hybridization
11. Fluorescence lifetime imaging
12. Homo-FRET measurements to investigate molecular scale organization of proteins in living cells
13. High-content and high-throughput screening
Appendix 1
List of suppliers
Index
2. Confocal or wide-field? A guide to selecting appropriate methods for cell imaging
3. Image quantification and analysis
4. Transmitted light imaging
5. Spectral imaging techniques for fluorescence microscopy
6. Measurement of protein motion by photobleaching
7. Imaging calcium and calcium-binding proteins
8. Total internal reflection fluorescence microscopy
9. An overview of three-dimensional and four-dimensional microscopy by computational deconvolution
10. Correlative microscopy using Tokuyasu cryosections: Applications for immunolabeling and in situ hybridization
11. Fluorescence lifetime imaging
12. Homo-FRET measurements to investigate molecular scale organization of proteins in living cells
13. High-content and high-throughput screening
Appendix 1
List of suppliers
Index
Related Information
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