Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual 

0-87969-765-2

978-0-87969-765-5

T. Friedmann and J. Rossi, ed., Cold Spring Harbor Laboratory Press, 2006, 793 pp., soft cover

PARTIAL TOC
1. Introduction
VIRAL VECTORS
2. Retroviral Vectors
3. Development of Lentiviral Vectors Expressing siRNA
4. HIV–2 Vectors in Human Gene Therapy: Design, Construction, and Therapeutic Potential
5. SIV Vectors as Vehicles for DNA Delivery
6. Production and Use of Feline Immunodeficiency Virus–based Lentiviral Vectors
7. Lentivirus Transduction of Hematopoietic Cells
8. Spleen Necrosis Virus–based Vectors
9. Foamy Virus Vector Production and Transduction of Hematopoietic Cells
10. Simian Foamy Virus Type–1 Vectors
11. Generation of VSV–G–pseudotyped Retroviral Vectors
12. Targeted Gene Transfer with Surface–engineered Lentiviral Vectors
13. Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement
14. Generation of 2A Peptide–linked Multicistronic Vectors
15. Construction of First–generation Adenoviral Vectors
16. Production and Characterization of Helper–dependent Adenoviral Vectors
17. Cell and Tissue Targeting
18. Stable Producer Cell Lines for AAV Assembly
19. Strategies for the Design of Hybrid Adeno–associated Virus Vectors
20. Recombinant Herpes Simplex Virus Vectors
21. Herpes Simplex Virus Type–1–derived Amplicon Vectors
22. γ–2 Herpesvirus Saimiri–based Vectors
23. Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors
24. Polyomaviruses: SV40
25.
26.
27.
28.
29.
30.
31.
32.
33.
NONVIRAL TECHNIQUES AND VECTORS
34. An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery
35. Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation
36. Gene Delivery to Skin Using Biolistics
37. Optimizing Electrotransfection of Mammalian Cells In Vitro
38. Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos
39. Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery
40. Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery
41. HVJ Liposomes and HVJ Envelope Vectors
42. Polylysine Copolymers for Gene Delivery
43. PEI Nanoparticles for Targeted Gene Delivery
44. Cyclodextrin–containing Polycations for Nucleic Acid Delivery
45. Bionanocapsules Using the Hepatitis B Virus Envelope L Protein
46. Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro
47. PEGylated Poly–l–lysine DNA Nanoparticles
48. Water–soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery
49. Cationic Polysaccharides for DNA Delivery
50.
51.
52.
53.
54.
55.
56.
57.
58.
59.
REGULATION OF TRANSGENE EXPRESSION
60. Conditional Mutagenesis of the Genome Using Site–specific DNA Recombination
61. Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells
62. Mifepristone–inducible Gene Regulatory System
63. Dimerizer–mediated Regulation of Gene Expression
64.
65.
66.
SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY
67. Assembly of De Novo Bacterial Artificial Chromosome–based Human Artificial Chromosomes
68. Delivery of Naked DNA Using Hydrodynamic Injection Techniques
69. Nonviral Gene Transfer across the Blood–brain Barrier with Trojan Horse Liposomes
70.
71.
72.
73.
TRANSGENIC APPROACHES
74. Pronuclear Microinjection in Mice
75. Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors
APPENDIX