| Product Name |
Luciferase from Photinus pyralis (firefly),
BioUltra, lyophilized powder, ≥98% (SDS-PAGE), 15-30 × 106 light units/mg protein |
| Product Number |
L9506 |
| Product Brand |
Sigma |
| CAS Number |
61970-00-1 |
| Storage Temp |
−20°C |
|
| TEST |
SPECIFICATION |
| APPEARANCE |
REPORT RESULT |
| PROTEIN BY UV ABSORBANCE |
APPROX. 20% |
| ENZYMATIC ACTIVITY |
15 TO 30MILLION LIGHT UNITS/MG PROTEIN |
| UNIT DEFINITION |
ONE LIGHT UNIT PRODUCES A BIOMETER PEAK HEIGHT EQUIVALENT TO 0.02 MICROCURIES OF 14C IN PPO/POPOP COCKTAIL. LIGHT UNITS ARE MEASURED IN 50MICROLITERS OF ASSAY MIXTURE CONTAINING 5PICOMOLES OF ATP AND 7.5MOLES LUCIFERIN IN GLYCINE-TRIS BUFFER, PH7.6 AT 25DEGC |
| ATPASE |
<5 NANOMOLAR UNITS/MG PROTEIN |
| NUCLEOSIDE DIPHOSPHOKINASE |
<20 NANOMOLAR UNITS/MG PROTEIN |
| SENSITIVITY |
LESS THAN OR EQUAL TO 1FEMTOMOLE OF ATP CAN BE DETERMINED USING 0.2MICROGRAMS OF LUCIFERASE AND SUITABLY SENSITIVE LIQUID SCINITILLATION COUNTERS OR LUMINOMETERS |
| RECOMMENDED RETEST |
1 YEAR |
|
DOCUMENT # L9506/02/21/02/3 |
Certificate of Analysis
Certificate of Origin
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