| Product Name |
Nuclease P1 from Penicillium citrinum,
lyophilized powder, ≥200 units/mg protein (E1%/280, 3'-5'-Phosphodiesterase) |
| Product Number |
N8630 |
| Product Brand |
Sigma |
| CAS Number |
54576-84-0 |
| Storage Temp |
2-8°C |
|
| TEST |
SPECIFICATION |
| APPEARANCE |
REPORT RESULT |
| PROTEIN BY UV ABSORBANCE |
REPORT RESULT |
| 3'-5'-PHOSPHODI- ESTERASE ACTIVITY |
> OR = 200UNITS/MG PROTEIN, > OR = 250 UNITS/VIAL (USING RNA SUBSTRATE) |
| UNIT DEFINITION |
ONE UNIT WILL LIBERATE 1.0MICROMOLE OF ACID SOLUBLE NUCLEOTIDES FROM RNA, R6625, PER MINUTE AT PH5.3 AT 37DEGC. |
| 3'-NUCLEOTIDASE ACTIVITY |
> OR = 1,000UNITS/MG PROTEIN (USING 3'-AMP SUBSTRATE) |
| UNIT DEFINITION |
ONE UNIT WILL HYDROLYZE 1.0MICROMOLE OF ORTHOPHOSPHATE FROM 3'-AMP PER MINUTE AT PH7.2 AT 37DEGC. |
| RECOMMENDED RETEST |
4 YEARS |
| DOCUMENT # |
N8630/12/18/07/6 |
Certificate of Analysis
Certificate of Origin
Related Information
Links
Page Options
