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PKH Fluorescent Cell Linkers

PKH Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects. Patented cell linker technology incorporates aliphatic reporter molecules into the cell membrane lipid bilayer by selective partitioning. PKH2 and PKH67 are green fluorochromes with excitation (490 nm) and emission (504 nm) similar to fluorescein, while PKH26, a red fluorochrome, has excitation (551 nm) and emission (567 nm) characteristics compatible with rhodamine or phycoerythrin detection systems. PKH26 may also be excited by the 488 nm emission of an argon-ion laser. Labeled cells retain both biological and proliferative activity, and are ideal for cell tracking and cell-cell interaction studies.
• Fluorescent labeling stable for up to 100 days on live cells
• Non-cytotoxic - no effect on biological or proliferative activity
• Versatile - labels various eukaryotic cells and cell lines, bacteria, and parasites
• No significant dye leakage or transfer from cell to cell
• Different dyes permit multi-color analysis

See www.sigma-aldrich.com/pkh for additional technical details on PKH Fluorescent Cell Linkers, including a comprehensive bibliography.


Standard Protocol for PKH Dye Labeling

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Confocal image of a Leishmania donovani promastigote labeled with PKH26 and expressing GFP....
PKH26-labeled young migrating neurons invading the developing cortex. (Photo courtesy of H. Wichte...

Product #
Description
MIDCLARET CellVue® Claret Far Red Fluorescent Cell Linker MIDI Kit New
MINCLARET CellVue® Claret Far Red Fluorescent Cell Linker MINI Kit New
GLDIL Diluent A For general membrane labeling
PCLDIL Diluent B for Phagocytic cell labeling
CGLDIL Diluent C For general membrane labeling
PKH2GL PKH2 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
PKH2PCL PKH2 Green Fluorescent Cell Linker Kit for Phagocytic Cell Labeling
PKH26GL PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling
PKH26PCL PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling
MINI26 PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
P7458 PKH26 Reference Microbeads
PKH67GL PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling
MIDI67 PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling New
MINI67 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
P2477 PKH67 Reference Microbeads

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