Human Genomic DNA

Human Random Control DNA Panels for use as reference standards in routine assays
Sigma-Aldrich and ECACC have teamed together to provide researchers with control populations of human genomic DNA for gene regulation and quantitative PCR research. The range of Human Random Control (HRC) DNA samples represents a control population of 480 UK Caucasian blood donors. The HRC DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) that can be continuously propagated in culture. This ensures an infinite supply of the unvarying DNA panels. The composition of each panel is completely defined and standardized so that each lot will be identical. Therefore, the HRC DNA Panels can be used as Reference Standards as routine quality control in the laboratory.

• Consistent Control Samples
  The DNA is extracted from immortalized cell lines held as cryopreserved banks, so there is no batch-to-batch variation.
• Convenient and Ready to Use
  Ideal for most standard genetic research applications and avoids errors in preparing controls.
• Cost Effective
  The 10 μg format provides enough purified HRC DNA for thousands of PCR assays.
• Format
  The purified HRC DNA is available in 5 different panels (HRC 1 through 5) consisting of 96 individuals with 10 μg each at a concentration of 200 ng/μl. For convenience, three of the panels (HRC1, HRC2, and HGDBC1) are available as a 96‑well format for use in PCR without the need for aliquoting or dilution at a concentration of 8 ng/μl.