SYBR^® Green Based Quantitative PCR

SYBR^® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma’s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated Taq polymerase, JumpStart Taq.

SYBR Green I Based QPCR Technical Animation