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Restriction Enzymes

Restriction Enzyme
Description
Product #
Acc I from Acinetobacter calcoaceticus buffered aqueous glycerol solution Recognition Sequence: 5'-GT/(A,C)(G,T)AC-3'
Ligation and recutting results: After 2-10-fold Acc I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity retained after 10 minutes at 65 °C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80 °C for 20 minutes.
 R6142
Alu I from Arthrobacter luteus buffered aqueous glycerol solution Recognition Sequence: 5'-AG/CT-3'
Ligation and recutting results: After 2-10-fold Alu I overdigestion of 1 μg λ DNA substrate, results in 95% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R6885
Apa I from Acetobacter pasteurianus buffered aqueous glycerol solution Recognition Sequence: 5'-GGGCC/C-3'
Ligation and recutting results: After 2-10-fold Apa I overdigestion of Ad-2 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R4258
Ava I from Anabaena variabilis buffered aqueous glycerol solution Recognition sequence: 5′-C/PyCGPuG-3′
Ligation and recutting results: After 2-10-fold Ava I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R3379
Ava II from Anabaena variabilis buffered aqueous glycerol solution Recognition sequence: 5′-G/G(A,T)CC-3′
Ligation and recutting results: After 2-10-fold Ava II overdigestion of 1 μg λ DNA substrate, results in 100%cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R6004
BamH  I from Bacillus amyloliquefaciens H buffered aqueous glycerol solution Recognition sequence: 5′-G/GATCC-3′
Ligation and recutting results: After 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95%recut.
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
 R0260
Bcl I from Bacillus caldolyticus buffered aqueous glycerol solution Recognition sequence: 5′-T/GATCA-3′
Ligation and recutting results: After 2-10-fold Bcl I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95% recut.
Heat inactivation: This enzyme cannot be heat inactivated at 65 °C for 15 minutes.
 R8631
Bgl II from Bacillus licheniformis buffered aqueous glycerol solution Recognition sequence: 5′-A/GATCT-3′
Ligation and recutting results: After 2-10-fold Bgl II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat Inactivation: This enzyme cannot be heat inactivated.
 R6377
Bgl I from Bacillus licheniformis buffered aqueous glycerol solution Recognition sequence: 5′-GCC(N)4/NGGC-3′
Ligation and recutting results: After 2-10-fold Bgl I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivation is achieved at 65 °C for 15 minutes.
 R6753
Bln I from Brevibacterium linens buffered aqueous glycerol solution Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.
 R3131
Bsm I from Bacillus stearothermophilus NUB 36 buffered aqueous glycerol solution Recognition sequences: 5'-GAATGCN/-3' 3'-CTTAC/GN 5
Ligation and recruiting results: After 2-10-fold Bsm I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Heat inactivation for Bsm I is not available.
 R3635
BssH II from Bacillus stearothermophilus buffered aqueous glycerol solution Recognition sequence: 5′-G/CGCGC-3′
Ligation and recutting results: After 2-10-fold BssH II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Sensitive and inactivated at 80 °C for 20 minutes.
Comment: Optimal activity is at 50 °C
 R2634
BstE II from Bacillus stearothermophilus ET buffered aqueous glycerol solution Recognition sequence: 5′-G/GTNACC-3′
Ligation and recutting results: After 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.
 R4253
Cfo I from Clostridium formicoaceticum buffered aqueous glycerol solution Recognition Sequence: 5′-GCG/C-3′
Ligation and recutting results: After 2-10-fold Cfo I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Not complete at 65 °C for 10 minutes.
 R1761
Cla I from Caryophanon latum L buffered aqueous glycerol solution Recognition sequence: 5′-AT/CGAT-3′
Ligation and recutting results: After 2-10-fold Cla I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Enzyme is not inactivated at 65 °C for 20 minutes.
 R7763
Dde I from Desulfovibrio desulfuricans strain Norway buffered aqueous glycerol solution Recognition sequence: 5′-C/TNAG-3′
Ligation and recutting results: After 2-10-fold Dde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Completed after 65 °C incubation for 20 minutes.
Comment: Single-stranded DNA cleaves slowly.
 R4256
Dpn I from Diplococcus pneumoniae buffered aqueous glycerol solution Recognition sequence: 5′-GmA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.
 R8381
Dra I from Deinococcus radiophilus buffered aqueous glycerol solution Recognition sequence: 5′-TTT/AAA-3′
Ligation and recutting results: After 2-10-fold Dra I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation: 65 °C for 20 minutes.
 R4381
EclX I from Enterobacter cloacae 590 buffered aqueous glycerol solution Recognition sequence: 5′-C/GGCCG-3′
Ligation and recutting results: After 2-10-fold EclX I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
 R3884
EcoR I from Escherichia coli BS5 buffered aqueous glycerol solution Recognition sequence: 5′-G/AATTC-3′
Ligation and recutting results: After 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
 R4640
EcoR I from Escherichia coli BS5 buffered aqueous glycerol solution Recognition sequence: 5′-G/AATTC-3′
Ligation and recutting results: After 2-10-fold EcoR I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
 R6265
EcoR II from Escherichia coli MV1 193 buffered aqueous glycerol solution Recognition sequence:5′-/CC(AT)GG-3′
Ligation and recutting results: After 2-10-fold EcoR II overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: 65 °C for 10 minutes.
 R1636
EcoR V from Escherichia coli buffered aqueous glycerol solution Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
 R2756
Hae III from Haemophilus aegyptius buffered aqueous glycerol solution Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
 R5628
Hind III from Haemophilus influenzae buffered aqueous glycerol solution Recognition sequence: 5′-A/AGCTT-3′
Ligation and recutting results: After 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R1137
Hpa I from Haemophilus parainfluenzae buffered aqueous glycerol solution Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.
 R8507
Hpa II from Haemophilus parainfluenzae buffered aqueous glycerol solution Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Hpa II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
 R0629
Kpn I from Klebsiella pneumoniae buffered aqueous glycerol solution Recognition sequence: 5′-GGTAC/C-3′
Ligation and recutting results: After 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R1258
Ksp I from Kluyvera sp. buffered aqueous glycerol solution Recognition sequence: 5′-CCGC/GG-3′
Ligation and recutting results: After 2-10-fold Ksp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
 R4134
Mlu I from Micrococcus luteus (lysodeikticus) buffered aqueous glycerol solution Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
 R8257
Msp I from Moraxella sp. buffered aqueous glycerol solution Recognition sequence: 5′-C/CGG-3′
Ligation and recutting results: After 2-10-fold Msp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R4506
Mva I from Micrococcus varians Rfl 19 buffered aqueous glycerol solution Recognition sequence: 5′-CC/(A,T)GG-3′
Ligation and recutting results: After 2-10-fold Mva I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut
Heat inactivation: Inactivated at 60 °C for 15 minutes.
 R1632
Nco I from Nocardia corallina buffered aqueous glycerol solution Recognition sequence: 5′-C/CATGG-3′
Ligation and recutting results: After 2-10-fold Nco I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R8761
Nde I from Neisseria denitrificans buffered aqueous glycerol solution Recognition sequence: 5′-CA/TATG-3′
Ligation and recutting results: After 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 60 °C for 15 minutes.
 R5509
Nhe I from Neisseria mucosa heidelbergensis buffered aqueous glycerol solution Recognition sequence: 5′-G/CTAGC-3′
Ligation and recutting results: After 2-10-fold Nhe I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >80% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R5634
Not I from Nocardia otidiscaviarum buffered aqueous glycerol solution Recognition sequence: 5′-GC/GGCCGC-3′
Ligation and recutting results: After 2-10-fold Not I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R8506
Nsi I from Neisseria sicca buffered aqueous glycerol solution Recognition sequence: 5′-ATGCA/T-3′
Ligation and recutting results: After 2-10-fold Nsi I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R5884
Pst I from Providencia stuartii buffered aqueous glycerol solution Recognition sequence: 5′-CTGCA/G-3′
Ligation and recutting results: After 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
 R7023
Pvu I from Proteus vulgaris buffered aqueous glycerol solution Recognition sequence: 5′-CGAT/CG-3′
Ligation and recutting results: After 2-10-fold Pvu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
 R1508
Pvu II from Proteus vulgaris buffered aqueous glycerol solution Recognition sequence: 5′-CAG/CTG-3′<
Ligation and recutting results: After 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.
 R2631
Rsa I from Rhodopseudomonas sphaeroides buffered aqueous glycerol solution Recognition sequence: 5′-GT/AC-3′
Ligation and recutting results: After 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R4756
SAC™ I from Streptomyces achromogenes buffered aqueous glycerol solution Recognition sequence: 5′-GAGCT/C-3′
Ligation and recutting results: After 2-10-fold Sac I overdigestion of 1 μg λ Hind III DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation : Inactivated at 65 °C for 15 minutes.
 R5268
Sal I from Streptomyces albus G buffered aqueous glycerol solution Recognition sequence: 5′-G/TCGAC-3′
Ligation and recutting results: After 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R0754
Sau3A I from Staphylococcus aureus buffered aqueous glycerol solution Recognition sequence: 5′-/GATC-3′
Ligation and recutting results: After 2-10-fold Sau3A I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.
 R0762
Sca I from Streptomyces caespitosus buffered aqueous glycerol solution Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.
 R5007
ScrF I from Streptococcus cremoris buffered aqueous glycerol solution Recognition sequence: 5′-CC/NGG-3′
Ligation and recutting results: After 2-10-fold ScrF I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.
 R7888
Sfi I from Streptomyces fimbriatus buffered aqueous glycerol solution Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Ligation and recutting results: After 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
 R8256
Sma I from Serratia marcescens Sb buffered aqueous glycerol solution Recognition sequence: 5′-CCC/GGG-3′
Ligation and recutting results: After 2-10-fold Sma I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >80% recut.
Heat inactivation: 65 °C for 15 minutes.
 R4503
Spe I from Sphaerotilus sp. buffered aqueous glycerol solution Recognition sequence: 5′-A/CTAGT-3′
Ligation and recutting results: After 2-10-fold Spe I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R5257
Sph I from Streptomyces phaeochromogenes buffered aqueous glycerol solution Recognition sequence: 5′-GCATG/C-3′
Ligation and recutting results: After 2-10-fold Sph I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R7135
Ssp I from Sphaerotilus sp. buffered aqueous glycerol solution Recognition sequence: 5′-AAT/ATT-3′
Ligation and recutting results: After 2-10-fold Ssp I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R6759
Stu I from Streptomyces tubercidicus buffered aqueous glycerol solution Recognition sequence: 5′-AGG/CCT-3′
Ligation and recutting results: After 2-10-fold Stu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R8013
Swa I from Staphylococcus warneri buffered aqueous glycerol solution Recognition sequence: 5′-ATTT/AAAT-3′
Ligation and recutting results: After 2-10-fold Swa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
 R1885
Taq I from Thermus aquaticus recombinant, expressed in Escherichia coli (Strain that carries a Taq I overproducing plasmid.), buffered aqueous glycerol solution Recognition sequence: 5′-T/CGA-3′
Ligation and recutting results: After 2-10-fold Taq I overdigestion of 1 μg λ DNA substrate, results in >95% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
 R9507
Xba I from Xanthomonas badrii buffered aqueous glycerol solution Recognition sequence: 5′-T/CTAGA-3′
Ligation and recutting results: After 2-10-fold Xba I overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Up to 15 units of enzyme inactivated at 65 °C for 15 minutes.
 R7260
Xho I from Xanthomonas holcicola buffered aqueous glycerol solution Recognition sequence: 5′-C/TCGAG-3′
Ligation and recutting results: After 2-10-fold Xho I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
 R6379

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