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Biotechnology books

Life Science and Chemistry books

Our Biotechnology books is a compilation of titles on Life Science techniques from affinity capillary electrophoresis to visualization of receptors.

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Image	Product #	Book Title	Description
	P2481	2-D Proteome Analysis Protocols	Leading investigators in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains.
	Z730394	Advanced Technologies in Biopharmaceutical Processing	An increasing number of pharmaceuticals in human and veterinary medicine are being developed using advanced genetic and other methods that focus on modification of somatic and embryonic cells. These methods call for new processes that go beyond the traditional unit processes of chemical and biological production. This book is the first to describe these advanced biological processes and show how they are applied to the production of biopharmaceuticals, from product generation and purification to fill-finish operations. The work explains how these technologies function similarly to unit operations for producing advanced biopharmaceuticals, such as hormones, modified proteins, etc. From large-scale animal cell bioreactors to patient-customized products, this volume guides users on how to apply new technologies in process development.
	Z702226	Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics	This reference presents recent breakthroughs and techniques in affinity capillary electrophoresis (ACE) to measure and determine the physicochemical and thermodynamic parameters of drug compounds—offering strategies to explore and characterize interactions between drugs, drug vehicles, and biological membranes to facilitate developments in controlled drug delivery and targeting. This book contains current applications and procedures to identify binding constants between drugs, biological structures, proteins, nucleic acids, and plasmids; increase drug bioavailability; enhance the permeability, solubility, strength, and function of drug formulations; examine protein-protein exchanges and reactions; calculate pK_a values.
	A5847	Affinity and Immunoaffinity Purification Techniques	Application of affinity and immunoaffinity ligands is an essential aid in the selective isolation of specific analytes. Techniques have nuances, such as ligand orientation, ligand stability, and bound analyte recovery, that can hinder successful isolation of the desired material and result in suboptimal or nonrecovery. Other reasons for failure are that separation science approaches an art form, and that tricks essential for success are lost in the text or missing from published articles. This is a sound guide for those wishing to use these techniques in their experimental designs.
	A6341	Automation Technologies for Genome Characterization	For end users of automated laboratory systems, this book will be a valuable reference for learning about appropriate technologies used in human genome characterization. It outlines how to access information about organizations and laboratories active in this field.
	Z719099	Basic Methods in Microscopy	Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
	Z719102	Basic Methods in Microscopy	Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
	Z702625	Bio conjugation Protocols: Strategies and Methods	Experts update the classic bioconjugation methods and introduce techniques that go beyond pure biomedical applications to include elements from organic synthesis, molecular biology, and materials science. These methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address on-chip synthesis to generate microarrays for the high-throughput analysis of RNA and DNA.
	Z704768	Biocatalysts and Enzyme Technology	A comprehensive overview of current knowledge of biocatalysis and enzyme technology. Following an introduction to the history of enzyme applications and the motivations for using these highly selective and environmentally friendly methods, the book goes on to cover enzyme mechanisms and kinetics, production, recovery, characterization and their design, including recombinant methods. Alongside the application of soluble and immobilized biocatalysts, including whole-cell systems, the authors treat the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. In line with the book's didactic approach, a number of case studies further exemplify the advantages of enzyme processes. Each topic includes exercises, designed to facilitate access to this area of research.
	Z510734	Biocatalysts for Fine Chemicals Synthesis	Includes a state-of-the-art review on biocatalysis followed by a collection of protocols covering areas where cells and enzymes have been proven to be useful catalysts. Contains fully tested and validated protocols and Step-by-step instructions for the expert and the inexperienced chemist
	Z374210	Bioconjugate Techniques	A practical guide to modification and cross-linking technology for research, diagnostics and therapeutics. Contains detailed information on the chemistry, reagent systems and practical applications for creating labeled or conjugated molecules.
	Z269662	Biodegradable Hydrogels for Drug Delivery	This book provides an in-depth investigation of recent advances in biodegradable hydrogels. Based on extensive research and experience, the book is the first to deal exclusively with this important area in drug delivery. A valuable guide for all, the book begins with a detailed introduction to current biodegradable hydrogel technology. Following chapters present advanced R&D, delivery systems, and more. It covers the mechanisms of biodegradation, types of biodegradable hydrogels, chemical and physical gels, chemical and enzymatic degradation, and examples of biodegradable drug delivery systems.
	Z379565	Bioluminescence Methods and Protocols: Methods in Molecular Biology, Vol. 102	A collection of methods for using luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, and cell-based assays. The contributors are those who have developed and perfected their methods. The wide variety of illustrative applications ranges from specific molecular titer to microbial detection and analysis. Other topics include the manipulation of the lux, luc, and gfp genes and construction of recombinant organisms. Cutting-edge techniques, detailed instructions, and diverse examples should make this book a standard reference.
	Z701106	Biotechnology and Biopharmaceuticals: Transforming Proteins and Genes into Drugs	This book defines biotechnology from the perspective of pharmaceuticals. The first section focuses on the process of transforming a biologic macromolecule into a therapeutic agent, while the second section provides a brief overview of each class of macromolecule with respect to physiological role and clinical application. Additional detail is also provided in the second section for each FDA approved, recombinantly derived biopharmaceutical for each category of macromolecule. The final section looks to the future and the new advances that will enhance our ability to develop new macromolecules into effective biopharmaceuticals. This last section discusses various drug delivery strategies while also describing gene and cell therapy strategies.
	B5160	Biotechnology from A to Z, 2nd ed.	Fully updated to incorporate the most currently terminology, this second edition offers an illuminating and practical introduction to the ideas of biotechnology. From ADEPT to zoonosis, this book provides a concise and readable overview of the field. It explains the usefulness of many techniques in this fascinating and controversial industry.
	Z373680	Biotechnology: A Laboratory Course, 2nd ed.	this second edition consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry. New exercises on PCR and western blotting have been added, as well as new procedures on large-scale plasmid isolation and DNA quantitation. The procedures and principles demonstrated are widely applicable in the biological sciences.
	Z702838	Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory	This book is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis.
	Z364347	Capillary Electrophoresis	This is a complete, practical guide to the principles, forms, and instrumentation of capillary electrophoresis. The material on method development will be especially useful to those who are new to the technique.
	Z369411	Capillary Electrophoresis Guidebook: Principles, Operation and Applications	Method development, quantitative procedures, optimization of precision and sensitivity, fraction collection and method validation are among the practical areas covered by this text. Specific techniques addressed include micellar electrokinetic capillary chromatography, capillary gel electrophoresis, advanced sampling techniques and electrochromatography.
	Z378135	Capillary Electrophoresis in Analytical Biotechnology	The unique focus makes this methods book especially valuable to life scientists. Applications of CE to peptides for mapping proteins, to recombinant-expressed proteins for analysis of post-translational modification, to glycoproteins and oligosaccharides, and to nucleic acids round out the practical sections. A balanced account of the theory of CE helps practitioners develop an appreciation for methods development and for the capabilities and limitations of the technique.
	Z702641	Capillary Electrophoresis of Proteins and Peptides	Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding
	Z379832	Capillary Electrophoresis: Theory and Practice, 2nd ed.	From the basic theory of separation and detection through real-world analytical problems, this up-to-date volume will appeal to students and everyday practitioners alike. Features new chapters on separation of inorganic ions and carbohydrates, as well as traditional biochemistry and biotechnology applications. Appendices provide lists of standard buffer solutions, instrument suppliers, and details of several student experiments.
	Z730661	Centrifugal Separations in Biotechnology	This book is the first devoted to centrifugal separation in biotechnology. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation.
	Z357421	Centrifugation: Essential Data Series	This volume provides the core data required to carry out any centrifugal separation, whether working with cells, organelles, or macromolecules. Contents include types of centrifuges and rotors; gradient choice, preparation, fractionation and analysis; and other useful information.
	Z702609	Chemical Genomics	The most comprehensive reference on this new and rapidly accelerating area of biotechnological innovation.This highly interdisciplinary reference/text identifies and elucidates the most influential developments in chemical genomics for improved research and advancement in a diverse array of fields ranging from proteomics, bioinformatics, and microfluidics to drug discovery and toxicology.
	Z702676	Chemoinformatics: Concepts, Methods and Tools for Drug Discovery	The authors explain the theory behind the crucial concepts of molecular similarity and diversity, describe the challenging efforts to use chemoinformatics approaches to virtual and high-throughput screening, and illuminate the latest developments in multidimensional QSAR analysis. Other topics of interest include the use of partitioning algorithms and classification methods for analyzing large compound databases, screening sets, and virtual screening for active molecules; different approaches to target class-specific library design; and the generation of a novel class of molecular surface properties descriptors that can be readily calculated from 2D representations of molecular structures.
	Z730688	Crystallography Made Crystal Clear 3rd ed.	This book is the most comprehensive and concise reference for beginning Macromolecular crystallographers, written by an expert in the field. Rhodes' uses visual and geometric models to help readers understand the mathematics that form the basis of x-ray crystallography. He has invested a great deal of time and effort on World Wide Web tools for users of models. Rhodes' personal CMCC Home Page also provides access to tools and links to resources discussed in the text. Most significantly, the final chapter introduces the reader to macromolecular modeling on personal computers-featuring SwissPdbViewer, a free, powerful modeling program now available for PC, Power Macintosh, and Unix computers. This updated and expanded new edition uses attractive four-color art, web tool access for further study, and concise language to explain the basis of X-ray crystallography, increasingly vital in today's research labs.
	Z703222	Crystallography Made Crystal Clear, 2nd ed.	This second edition explains how scientists discover the structures of the macromolecules. Scientists do not see these molecules directly. Instead, they build models as a means of interpreting data from x-ray diffraction by crystals, or by irradiation by other forms of energy. Users of these models need to know how they are obtained in order to know what they are seeing when they study a model of a macromolecule. They also need to know how to judge whether conclusions they draw from the molecular models are really supported by the models. This book uses visual and geometric models to help readers understand the mathematics that forms the basis of x-ray crystallography. The field of protein crystallography has been instrumental in discovering the molecular principles of biology and in discovering new drugs.
	C5231	Cytoskeleton Methods and Protocols	Recent advances in experimental instrumentation, reagents and imaging technology have expanded the range of tools available for the study of the cytoskeleton. This book details reproducible methods for investigating cytoskeleton structure and function utilizing diverse model systems in a variety of organisms. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. Help notes and tips accompany each protocol and provide additional information.
	E8025	Electron Microscopy Methods and Protocols	Experts describe key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological x-ray microanalysis, this book includes methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Immunogold labeling techniques for ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in detail to provide insight into their advantages and limitations.
	E8890	Electron Microscopy: Principles and Techniques for Biologists. 2nd ed.	A direct, uncomplicated introduction to electron microscopy for biologists. This second edition has enhanced layout, illustrations, and text so that it is more accessible to the reader. Over 200 new line drawings and micrographs have been added.
	Z703575	Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, 4th ed.	This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
	E8778	Enzyme Assays: A Practical Approach, 2nd ed.	This edition combines revised chapters with new contributions in describing the design and execution of enzyme assays. Topics include protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. Theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high-throughout screening; statistical analysis of enzyme kinetic data; and determination of active site concentration
	E3903	Enzyme Technologies for Pharmaceutical and Biotechnological Applications	This review of enzyme function in human and animal health covers basic principles and applications in antibiotic biosynthesis, biocatalysis, screening and assay optimization, and new technologies. It offers examples of laboratory, pharmaceutical, and manufacturing processes. Emphasizing modern methods of metabolic engineering and drug discovery processes, it details enzyme targets related to human disease, development of effective enzymatic assays, methodologies in high-throughput screening, discovery of novel compounds for lead generation, improvements in antibiotic yield, and generation of contemporary protein products.
	E7154	Essentials of Genomics and Bioinformatics	Genomics is a new discipline that is revolutionizing the way biological and medical research is done. The human genome is almost completely sequenced, which will help to accelerate research and development in Genomics even more. The chapters in this book capture the rapidly evolving field. Overviews of nucleic acid technologies, proteomics and bioinformatics are followed by chapters on applications, including human health, agriculture and comparative genomics. The book also includes two chapters on legal and ethical issues of Genomics, a topic that is becoming more and more important.
	Z700223	FISH Technology	Fluorescence in situ hybridization (FISH) is a powerful method to directly visualize the localization of genomic alterations in the nucleus. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; FISH-related techniques, such as PRINS and microarrays.
	Z700940	FISH, A Practical Approach	This laboratory manual covers the theory and application of the commonly used FISH procedures. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.
	F0178	Flow Cytometry: First Principles, 2nd ed.	With advances in flow cytometry, this edition provides an update, reflecting innovations while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. It explains the basic principles of flow cytometry, surveys scientific and clinical applications and highlights state-of-the-art techniques. This edition contains revisions of all chapters with new discussions on fluorochrome and laser options for multicolor analysis, an additional section on apoptosis in the chapter on DNA, and new chapters on intracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology.
	F3426	Fundamental Laboratory Approaches for Biochemistry and Biotechnology	Written by active scientists, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. It features 13 chapters on principles of biochemistry and molecular biology techniques, and 26 laboratory experiments covering basic methods. All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation. Unique chapter on using the Web for biochemistry research is provided along with a comprehensive Website that supports the book. (http://www.umich.edu/~aninfa/book/)
	F9927	Fundamentals of Light Microscopy and Electronic Imaging	Researchers need new skills in order to use a modern research microscope, such as how to align microscope optics and perform image processing. This book explores the basics of microscope design and use. It discusses optical principles involved in diffraction and image formation in the light microscope, basic modes of light microscopy, components of modern electronic imaging systems, and image processing operations necessary to acquire and prepare an image. It also reviews such topics as Illuminators, filters, and isolation of specific wavelengths; Phase contrast and differential interference contrast; Fluorescence and confocal laser scanning microscopy; Digital CCD microscopy and image processing.
	Z357413	Gel Electrophoresis: Essential Data Series	This volume presents comprehensive data on gel electrophoresis of proteins, nucleic acids, nucleoproteins and carbohydrates. Also presented are sections on electrophoresis chemicals, types of apparatus, troubleshooting, and manufacturers and suppliers.