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Cell Culture books

Best Books for the Best Minds

Our Cell Culture books provides a variety of methods resources that include topics such as stem cells and gene therapy.

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Image	Product #	Book Title	Description
	Z703036	Adult Stem Cells	This comprehensive review of our current understanding of adult stem cell types and their regulation examines the derivation, characterization, and utility of adult stem cells found in skeletal muscle, dental pulp, prostate, kidney, the eye, inner ear, and other tissues. The authors pay special attention to adult stem cell plasticity and their utility in normal tissue homeostasis, as well as their applications in regenerative medicine.
	Z730580	Animal Cell Biotechnology: Methods and Protocols, 2nd ed.	This second edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques.
	Z702943	Animal Cell Culture and Technology, 2nd ed.	This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed. New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use
	A0687	Animal Cell Culture: A Practical Approach, 3rd ed.	This edition includes new or updated chapters on: cell authentication; serum-free culture; apoptosis assays; FISH; genetic modification; stem cell assays; 3-dimensional culture; tissue engineering; cytotoxicity assays; scaling-up of cell culture. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible
	Z702919	Basic Cell Culture Protocols 3rd ed.	This third edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research
	B2183	Basic Cell Culture: A Practical Approach, 2nd ed.	This book is a guide to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced and novice researchers should find this book useful as a source of reference, when entering a new field, or when setting up a new laboratory.
	Z705292	Cell Culture Technology for Pharmaceutical and Cell-Based Therapies	This reference presents a comprehensive overview of the state-of-the-art in cell culture technology. Each expertly written chapter covers research, principles, and equipment currently affecting advances in the discovery of genes, host cell selection, cloning and gene amplification, bioreactor design, protein purification, optimization and scale-up, product quality, and facility design--showcasing the importance of genomic and cell-based technologies in the pharmaceutical industry. Providing clear sections on aspects of cell biology and biochemical engineering used in pharmaceutical process development and manufacturing, this source describes manipulations and the genetic engineering of cells for the production of biotechnology products
	Z340669	Cell Growth, Differentiation and Senescence: A Practical Approach	This book provides a combination of succinctly expressed basic concepts of cell growth and cell death with detailed instructions and protocols on how to accurately measure these processes. Practical instructions are accompanied by explanatory material, which allows a researcher to choose which protocol is best for their purpose. Methods outlined range from simple techniques, such as autoradiography and cell staining, to more complex techniques, such as flow cytometry.
	Z718890	Cell Growth: Control of Cell Size	Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division
	Z702528	Cell Growth: Control of Cell Size	Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
	Z703877	Cell Migration: Developmental Methods and Protocols	A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
	C0983	Cell Movements: From Molecules to Motility, 2nd ed.	Interiors of cells are busy with purposeful movement, as vesicles are transported from one location to another and chromosomes align and then separate during cell division. What powers these movements? How are they controlled and made responsive to external stimuli? This book describes how complex movements can arise from properties and behaviors of biological molecules. Biophysical parameters are highlighted, emphasizing importance of diffusion, viscosity, and mechanical forces in determining cellular responses.
	C9862	Cell Physiology Sourcebook: A Molecular Approach, 3rd ed.	This authoritative book gathers together a broad range of ideas and topics. It provides clear, concise, and comprehensive coverage of cellular physiology from fundamental concepts to more advanced topics and contains substantial new material. The book includes chapters on sensory transduction, the physiology of protozoa and bacteria, the regulation of cell division, and programmed cell death. Features include eight new chapters on such topics as membrane structure, intracellular chloride regulation, transport, sensory receptors, pressure, and olfactory/taste receptors; broad coverage of both animal and plant cells.
	C4724	Cell Separation: A Practical Approach	The successful separation of specific cells requires not only a knowledge of potential methods, but also detailed protocols that are known to work well in practice. This book is unique in that it brings together the best methods currently used for separating cells. For each approach, the authors provide clear explanations of the theoretical basis of the procedures as well as practical details of the technique. All researchers for whom cell separation is the starting point for their experimental work should read this book. As such, it is an invaluable laboratory manual for cell biologists, biochemists, molecular biologists, and physiologists.
	C9605	Cell and Tissue Culture for Medical Research	This is a collection of fundamental and specific applied procedures in cell and tissue culture for clinical research including those that form the basis for the new medical techniques of tissue engineering and gene therapy. It includes step-by-step protocols with troubleshooting tips, experimental examples to indicate expected results, and alternative procedures.
	C8485	Cell-Cell Interactions: A Practical Approach, 2nd ed.	The book comprises ten chapters written by experts in the field on cell-cell interactions. These areas have been selected for their breadth and relevance where these interactions have been shown to play a critical role in biological processes. Chapters include methodologies for specialized cell types, specialized domains of membrane contact, different developmental models and mammalian development.
	Z703990	Culture of Animal Cells: A Manual of Basic Techniques, 5th ed.	This fifth and fully revised edition of Culture of Animal Cells continues to provide the most comprehensive information on the techniques, equipment, principles, and practices of animal cell culture. It offers a complete background related to growth of animal cells in tissue culture, beginning with laboratory design, safety, and bioethics, then continuing to coverage of preparation through to contamination, molecular techniques, and troubleshooting.
	Z705314	Culture of Cells for Tissue Engineering	Specific applications of tissue engineering include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. This book offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
	C3612	Culture of Epithelial Cells, 2nd ed.	This edition, devoted to those cells that line the body protecting surfaces and secreting or absorbing material, provides revised descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. Each chapter is devoted to a different type of epithelium--epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. Freshney contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form. Other features include alternate techniques coupled with critical comparative analyses and new chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium.
	C5228	Culture of Hematopoietic Cells	Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.
	Z701696	Culture of Human Tumor Cells	This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. It is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed.
	D7687	DNA Transfer To Cultured Cells	DNA Transfer to Cultured Cells describes updated methods and applications for transferring genetic material to cells in-vitro. Advances achieved in recent years to develop approaches for introducing DNA into cells have facilitated studies on the mechanism of expression of different important genes in the cells. Consequently, it has been possible to evaluate the role of different genes and their activation in a variety of important processes, such as growth and uncontrolled proliferation (cancer). Different cell types require different schemes for DNA transfer; this book describes these diverse methods, their application to specific cell types and their relevance to a biological problem.
	Z700495	Dendritic Cells 2nd ed.	This new edition substantially revises and expands the 1999 work with a section of color plates and new information on the interaction of dendritic cells and viruses, the role of dendritic cells in wound healing and atherosclerosis, delivery of apoptotic bodies, genetic engineering of dendritic cells, imaging, and practical aspects of clinical protocol development. The 45 chapters discuss the origin and molecular biology of dendritic cells, their interaction with other cells, and their roles in cancer, allergy, and infections. With the volume of literature on dendritic cells almost doubling every year, this book provides the most up-to-date synthesis of the literature, written by the very best authors.
	Z718327	Development of Human Gene Therapy	The idea of human gene therapy was accepted by the medical community and society at large long before believable clinical benefits began to emerge. In this book, some of the field’s most distinguished contributors chronicle the evolution of this momentous direction for medicine, illustrating how imaginative concepts shaped the development of technologies and brought the daring new idea to its current position of imminent practical success. This is a book designed to endure as clinical advances accumulate, a clear-eyed work of reference that will anchor the further development of this revolution in therapy. It is an essential addition to libraries of clinical medicine, biotechnology, and public policy, and a resource that no laboratory investigator with an interest in the biology of gene transfer should be without.
	C4849	Dynamics Of Cell Division	This volume focuses on the structural aspects of cell division, ranging from nuclear envelope breakdown to cytokinesis and partitioning of the cytoplasm. It examines spindle assembly and chromosome behavior in mitosis and meiosis, centromere and kinetochore structure and regulation, telomeres, the role of centrosomes, and mechanisms by which overall regulation is achieved. Written as a companion volume to Cell Cycle Control, this book provides an up-to-date account of developments in this exciting area of cell biology.
	E1404	Embryonic Stem Cells: Methods and Protocols	This book describes productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. These detailed protocols range from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, to the generation of antibodies against very rare antigens, and to the identification of and characterization of protein and protein interactions. This will prove an invaluable resource for those actively using, or planning to use ES cells, to study fate choices and specific lineages.
	E2029	Epithelial Cell Culture Protocols	A team of experimenters and researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria.
	Z369950	Epithelial Cell Culture: A Practical Approach	Cells in culture can sometimes organize into tissue-like structures. In vitro epithelial ¿tissue¿ serves as a useful model system for studying pharmacology, toxicology, and cell biology. Written by an international panel of researchers, this book details the methods for establishing such a culture and describes some interesting research applications.
	Z700223	FISH Technology	Fluorescence in situ hybridization (FISH) is a powerful method to directly visualize the localization of genomic alterations in the nucleus. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; FISH-related techniques, such as PRINS and microarrays.
	G3918	Gene Therapy Protocols, 2nd ed.	In this revised edition experts detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. This edition offers a compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step procedures that enable its successful therapeutic application.
	Z702617	Gene and Cell Therapy: Therapeutic Mechanisms and Strategies, 2nd ed.	This edition offers current data on delivery systems and therapeutic strategies, gene expression and detection, and disease targets and therapeutic strategies. It presents results and prospects in gene therapy clinical trials, other cell-based therapies and trials, and regulatory issues. Topics include optimization of non-viral gene therapeutics, gene therapy for cancer, and DNA vaccines.
	H6033	Haematopoietic and Lymphoid Cell Culture	This reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact haematopoietic and lymphoid cells, and their maintenance as primary cultures. It provides technical information on the signals and mediators required for the differentiation and growth of these cells. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and culturing of murine thymic explants.
	Z700509	Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells	The authors describe the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.
	Z702927	Human Cell Culture Protocols 2nd ed.	A revised and updated collection readily reproducible techniques for culturing human cells. This edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine¿ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
	H8786	Human Cytogenetics: Constitutional Analysis, A Practical Approach, 3rd ed.	This covers all basic aspects of human cytogenetic study other than malignancies and abnormalities. Since the 2nd edition, the largest new development has been the transition of fluorescence in situ hybridization to an essential tool for cytogenetecists. Its use in chromosome analysis is covered in detail. Another new technology described in detail is computerised image analysis. Conventional techniques have not been forgotten, with chapters on chromosome staining and banding techniques and meiotic studies. New authors take a fresh look at lymphocyte culture and prenatal diagnosis
	H8911	Human Cytogenetics: Malignancy and acquired abnormalities, A Practical Approach, 3rd ed.	The advent of molecular technologies has lead to a rapid acceleration in the cytogenetic study of malignancy and acquired abnormalities. As well as having a chapter devoted to molecular technologies (FISH, PRINS, and CGH), molecular methodology is emphasized in all chapters. There are three chapters on the specific methodology for the cytogenetic study of myeloid leukemia, acute lymphoblastic leukemia, and lymphomas and lymphoproliferative disorders. Also covered are chromosome instability syndromes, mutagen-induced chromosome damage in lymphocytes and the role of cytogenetics in the assessment of hematological disorders.
	Z704288	Human Embryonic Stem Cells	This text begins by addressing the basic biology of the cells, in the context of the other stem cell populations present in fetuses and adult organisms. Discussion then turns to how stem cells can be used to further the understanding of human developmental processes, which currently lags far behind that of model organisms. Several chapters describe attempts to generate stem cell derived specific lineages as potential tools for cell based therapeutics, and these are followed by discussion of the legal and moral issues which accompany this ground breaking research.
	Z701408	Human Embryonic Stem Cells	A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.