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Image	Product #	Book Title	Description
	Z700320	DNA Array Image Analysis: Nuts & Bolts	DNA Microarrays are an indispensable tool in molecular biology, which allows researchers to gain insight into the intricate mechanisms of gene expression. Common to all array-based technologies is the necessity to analyze digital images of the scanned DNA array. This book emphasizes aspects of both theory and application. It offers the basics of machine vision and image analysis for biologists, "how-to" approach to microarray image analysis, and practical tips for both the novice in the field and the advanced user.
	D6064	DNA Arrays: Methods and Protocols	Microarray technology is used in research of gene expression, mutation analysis, proteomics and gene sequencing. Protocols provide instruction in designing and constructing DNA arrays as well as hybridizing them with biological samples for analysis. Details include how to attach or print arrays on various matrices, biological sample preparation (DNA and RNA), hybridization conditions, signal detection, probe optimization, different printing technologies and data collection and analysis. Other topics are genotyping, sequencing by hybridization, antisense reagents, gene expression analysis, and business aspects of biochip technology.
	D5565	DNA Arrays: Technologies and Experimental Strategies	A giant leap in technology took place with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. This book offers a view of different aspects of this rapidly developing technology, including oligonucleotides and electrochemical arrays. Up-to-date insights into methodological fundamentals of construction of DNA arrays, optimization of hybridization conditions, the use of expression data in bioinformatics, different software tools, and the application of microarray technology for drug discovery process are provided.
	D4816	DNA Chromatography	This book describes DNA Chromatography a powerful and effective tool for the molecular biologist researcher. The technology is useful for a variety of applications including the analysis of mutations (SNPs, insertions, and deletions), the size determination of PCR products, the purification of single- and double-stranded nucleic acids, analysis of enzymatic reaction products and many others. DNA Chromatography is automated and can replace many of the gel electrophoresis technologies being used resulting in faster and more accurate analysis.
	Z362360	DNA Cloning: A Practical Approach, 2nd ed. Volume 1: Core Techniques	Topics include transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and gene expression, in vitro mutagenesis, and DNA sequencing.
	Z362379	DNA Cloning: A Practical Approach, 2nd ed. Volume 2: Expression Systems	This volume provides essential information and full details of current procedures for the expression of cDNA clones in E. coli, lambda-phage, yeast, and baculovirus expression systems. Also covered are the isolation and purification of recombinant protein(s), and the generation of monoclonal and polyclonal antibodies.
	Z306193	DNA Cloning: A Practical Approach, 2nd ed. Volume 3: Complex Genomes
	Z306207	DNA Cloning: A Practical Approach, 2nd ed. Volume 4: Mammalian Systems	Topics covered include: high efficiency gene transfer into mammalian cells, use of vectors based on gene amplification, retroviral vectors, genetic manipulation of embryonic stem cells, production of transgenic rodents, genomic and expression analysis of transgenic animals, expression using a defective HSV-1 vector system, and adenovirus vectors.
	D4691	DNA Methylation Protocols	This book offers a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.
	Z704199	DNA Methylation: Approaches, Methods and Applications	A guide to understanding the complex regulatory roles of DNA methylation, this volume describes the relation that this biological mechanism has to gene silencing in disease and explores its promising role in treating cancer. The book is a state-of-the-art reference for understanding the role DNA methylation plays in human disease, particularly cancer. This compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation status of genomic DNA. It includes concepts, experimental models, and clinical uses of demethylating agents. The book provides a balance between articles clarifying methodological details and more general review chapters.
	D5440	DNA Microarrays: A Molecular Cloning Manual	DNA microarray, or DNA chip technology is a new and powerful means of discovering, characterizing and analyzing genes and their expression patterns. This manual assembles and synthesizes the expertise of over 30 innovators in this emerging field to provide authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for extensive image and data analysis. It complements the best selling manual "Molecular Cloning".
	D6187	DNA Microarrays: A Practical Approach	Written and edited by experts in the field, this book provides valuable insight into the remarkable advancement of DNA microarrays. It is essential for researchers investigating patterns of gene expression and useful for all those interested in the use of biochips.
	Z700185	DNA Microarrays: Gene Expression Applications	This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but tricky methods. Software, data mining procedures and probable future developments, which should be useful to anyone interested in expression measurement, are also covered. It provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.
	Z719293	DNA Microarrays: Methods Express Series Hardcover	DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology and its applications. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. THIS BOOK WILL BE PUBLISHED IN THE FUTURE, SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULLFILLMENT WHEN PUBLISHED.
	Z719307	DNA Microarrays: Methods Express Series Softcover	DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology and its applications. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. THIS BOOK WILL BE PUBLISHED IN THE FUTURE, SIGMA-ALDRICH CAN ACCEPT ORDERS NOW FOR FULLFILLMENT WHEN PUBLISHED.
	D7937	DNA Profiling and DNA Fingerprinting	This book reflects practical approaches to answering a variety of biological and medical questions using DNA fingerprinting and genetic profiling in a broad sense that also includes statistical evaluation of the data. It has been written for all researchers in the theoretical and applied sciences wherever genetic identification and relationship analysis are required.
	Z704784	DNA Repair : Mammalian Systems, 2nd ed.	This collection of reproducible techniques for repairing mammalian DNA contains 14 entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of “adducted” bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting.
	D6561	DNA Repair Protocols: Eukaryotic Systems	Instructions are provided for analyzing DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. Methods focus on eukaryotic model systems that have made, or may make, contributions to the understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and cell cycle. Non-mammalian systems as yeast, C. elegans, Drosophila, Xenopus, and plants are considered.
	D8436	DNA Repair Protocols: Prokaryotic Systems	This is a collection of protocols from leaders in the field. First section deals with identification, purification and characterization of DNA repair enzymes. Repair pathways include base excision repair, mismatch repair and recombination repair as it explores activities and characterization of specific DNA repair proteins. Second section provides protocols for applied use of DNA repair proteins in molecular biology techniques including mutation detection, cloning and genome diversification.
	Z369888	DNA Repair and Mutagenesis	A major reference work for molecular biologists working in carcinogenesis, gene regulation, environmental studies, and molecular evolution. The text details the mechanisms by which DNA can be damaged and the strategies that organisms have developed to mitigate the effects of that damage. The concluding chapter discusses human diseases that result from defective DNA repair processes.
	Z702501	DNA Repair in Cancer Therapy	The authors explain in detail the various mechanisms by which cancer cells can circumvent anticancer therapy. They also review the clinical impact of such novel inhibitors of DNA repair mechanisms as methylguanine-DNA-methyltransferase. Also examined are inhibitors of other DNA repair enzymes such as PARP and DNA-PK, now under development and close to clinical trials. The book captures for both cancer researchers and practicing oncologists dealing with hallmark "relapse" or "drug resistance" phenomena on a daily basis-the many exciting new uses of DNA repair inhibitors, either alone or in combination with anticancer therapies
	Z378437	DNA Replication in Eukaryotic Cells	An international panel of contributors combine to create this exhaustive treatise. General topics include origins of replication, priming, role of transcription factors in replication, fidelity and repair, chromatin structure, and comparisons to prokaryotic mechanisms. A section on proteins includes chapters on cellular and viral DNA polymerases, accessory proteins, ligases, topoisomerases, and telomerases. Finally, about half the book is devoted to discussion of specific replication systems including eleven viruses, yeast, insects, plants, mammals, organelles, and more.
	Z705462	DNA Sequencing II: Optimizing Preparation and Cleanup	Kieleczawa's second volume is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Clean-up of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a great addition to your molecular biology library.
	D4814	DNA Sequencing Protocols, 2nd ed.	Major advances have made PCR-based semiautomated fluorescent sequencing the norm. This new edition provides up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. It offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. It offers easy-to-follow methods that will improve the accuracy and quality of DNA sequences obtained by smaller laboratories and help lay the foundation for molecular diagnostics.
	Z373850	DNA Sequencing Strategies	With more than 100 charts, tables, and photographs, this book encompasses many important advancements and developments. Designed as an easy to use bench reference, it guides you through step-by-step sequencing processes, refining techniques, and research requirements.
	Z702870	DNA Sequencing: Optimizing the Process and Analysis	DNA sequencing techniques have evolved rapidly in recent years, but scientists still encounter challenges in sequencing genomes. This book was written to help troubleshoot these difficulties while also providing a practical guide for those routinely sequencing DNA to refine and enhance their operation. This volume covers important topics in the field, including: biochemical and technological advances induced by the Human Genome Project; proven and newly emerging methods of preparing DNA templates; effects of some widely used laboratory reagents on DNA sequencing; Laboratory Information Management Systems (LIMS); and the future of DNA sequencing.
	D9313	DNA Topoisomerase Protocols	This is a collection of protocols for investigating DNA structure, topology, and DNA topoisomerase function. These methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. Protocols detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases.
	D7687	DNA Transfer To Cultured Cells	DNA Transfer to Cultured Cells describes updated methods and applications for transferring genetic material to cells in-vitro. Advances achieved in recent years to develop approaches for introducing DNA into cells have facilitated studies on the mechanism of expression of different important genes in the cells. Consequently, it has been possible to evaluate the role of different genes and their activation in a variety of important processes, such as growth and uncontrolled proliferation (cancer). Different cell types require different schemes for DNA transfer; this book describes these diverse methods, their application to specific cell types and their relevance to a biological problem.
	Z340715	DNA Viruses: A Practical Approach	This book groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Topics include virus genome mapping, sequence analysis,, viral cytokine-binding proteins, analysis of virus replication, and neoplastic transformation.
	Z705136	DNA Viruses: Methods and Protocols	A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, this book will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
	D2936	DNA-Protein Interactions: A Practical Approach	This is a novel compilation of methods for studying the interactions of proteins with DNA. Multidisciplinary approaches are valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. It covers all of the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA, with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure.
	D6689	DNA-Protein Interactions: Principles and Protocols, 2nd ed.	Revised second edition provides the only book exclusively concentrating on up-to-date techniques for protein-DNA interactions. Easy-to-follow protocols illuminate the molecular basis of protein-nucleic acid interactions. Use them successfully to reveal location of DNA binding site, strength and specificity of a binding, identities of individual groups on actual bases involved in binding, and specific amino acid residues of the protein that interact with DNA. Some techniques can be used to identify previously unknown DNA binding proteins from crude cell extracts.
	Z705721	Data Analysis and Presentation Skills: an Introduction for the Life and Medical Sciences	Undergraduate and post-graduate students in the life sciences are required to research scientific information using a variety of resources. They are then required to use this information to design experiments, generate results, analyse their data and ultimately present this information to academics and referees. The title provides students with the opportunity of developing key skills within several areas enabling them to use such skills as research and presentation tools. • A hands-on approach introducing essential ICT skills required for bioscientists to work through final year projects and postgraduate study • Includes numerous relevant examples and case studies relevant to students within the biosciences
	Z703362	Dead Ends and Detours: Direct Ways to Successsful Total Synthesis	In almost all publications, the valuable information provided is almost always based on successful organic reactions. However, a chemist's life is not that easy. Trial and error is still one of the most commonly used methods. Thus it would be useful to have access to those syntheses that do not work, since they also provide important results of great importance for further synthesis. This book fills just such a gap. Using major total syntheses, the authors explain a variety of problems and recommend ways out of such dilemmas: Problems at the start and end of a synthesis, difficult and unexpected reactivities of functional groups, problems due to steric properties and much more. The result is a wealth of information for any organic chemist and an essential tool for successful total synthesis.
	Z702560	Dean’s Analytical Chemistry Handbook, 2nd ed.	This reference provides coverage of the full range of analytical methods, including all conventional wet and instrumental techniques. It also provides information on the preliminary operations of analysis, preliminary separation methods, quality control and quality assurance, and statistics in chemical analysis. This resource presents fundamental data on subjects such as: Volumetric and Gravimetric Analysis; Chromatographic Methods; Electronic Absorption and Luminescence Spectroscopy; Optical Activity and Rotary Dispersion; Nuclear Magnetic Resonance Spectroscopy and Electron Spin Resonance; Electroanalytical Methods; Thermal Analysis; Detection and Determination of Functional Groups in Organic Compounds; Water Analysis. It also contains new chapters on: Pesticide and Herbicide Analysis, and Environmental Analysis.
	Z558427	Dean’s Handbook of Organic Chemistry	This book updates biopolymers, electronegativity, nomenclatures, properties tables, and structural drawings making the data easier for the researcher to access and use.
	Z552216	Degradable Polymers: Principles and Applications, 2nd edition	Discussion on composting as a means of recovering value from wastes has led to a new impetus to understand how plastics degrade in the environment and the implication of this for international standards. Polymers based on renewable resources are also a major topic in this edition but the debate continues about their long-term sustainability and ecological advantages over degradable man-made polymers.
	Z513660	Dendrimers and Dendrons: Concepts, Syntheses, Applications	The book gives a comprehensive, up-to-date account of the topic, from the historical overview and theoretical background up to the most recent achievements. Includes sections on divergent and convergent synthesis, chiral dendritic macromolecules, metallodendimers, and much more. For special synthetic problems, the well-selected, detailed list of references allows easy access to further literature.
	Z514039	Dendrimers and other Dendritic Polymers	Provides a detailed insight into dendritic polymers, and discusses all the known subclasses of dendritic polymers in addition to dendrons and dendrimers, including hyperbranched polymers, dendrigrafts and megamers. With contributions from many of the leading scientists in the field of dendritic polymers, this comprehensive volume provides an overview of developments in the field of dendrimers, with a comparison of properties and synthesis to traditional polymers. Also includes a discussion of commercial and potential applications for dendritic polymers, an identification of the key trends and analytical perspectives in dendrimer research , and practical procedures for the laboratory preparation of some of the more commonly used dendrimer families. This will be essential reading for all chemists, polymer/material scientists, plastics engineers, nanotechnologists and postgraduate polymer scientists and engineers.
	Z700495	Dendritic Cells 2nd ed.	This new edition substantially revises and expands the 1999 work with a section of color plates and new information on the interaction of dendritic cells and viruses, the role of dendritic cells in wound healing and atherosclerosis, delivery of apoptotic bodies, genetic engineering of dendritic cells, imaging, and practical aspects of clinical protocol development. The 45 chapters discuss the origin and molecular biology of dendritic cells, their interaction with other cells, and their roles in cancer, allergy, and infections. With the volume of literature on dendritic cells almost doubling every year, this book provides the most up-to-date synthesis of the literature, written by the very best authors.
	Z730017	Deoxynucleoside Analogs in Cancer Therapy	This book expertly summarizes the current status of development and