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P2481 2-D Proteome Analysis Protocols Leading investigators in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains.
Z730300 PCR 2nd ed. A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
P4609 PCR Applications: Protocols for Functional Genomics From ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences, PCR Applications examines the latest developments in this field. It includes statistical refinement of primer design parameters, techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology.
P8117 PCR Cloning Protocols, 2nd ed. This edition update and expands Bruce White′s best-selling “PCR Protocols” (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long PCR and GC-rich template amplification. Powerful applications of PCR in library construction and sublibrary generation and screening are presented.
P8867 PCR Mutation Detection Protocols This book provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included.
Z701270 PCR Primer: A Laboratory Manual, 2nd ed. This second edition of a much praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered in protocols that have the hallmark reliability of the previous edition.
Z369918 PCR Protocols for Emerging Infectious Diseases The spectres of completely new infectious diseases and multiple drug-resistant pathogens are frightening reminders of our frailty. Modern PCR techniques provide the best methods for identifying the causes. This book presents 12 detailed protocols that can serve as models for development of new protocols when they are needed.
Z701351 PCR Protocols, 2nd ed. This edition has updated and expanded the number of protocols to take advantage of the newest technologies. These methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and a discussion of the interpretation and use of results.
P8177 PCR Protocols: A Guide to Methods and Applications The polymerase chain reaction (PCR) is a powerful new molecular biological method with widespread applications in genetics and disease diagnosis. This manual details current PCR methods.
Z373818 PCR Sequencing Protocols This book offers a comprehensive collection of the most reliable protocols for PCR Sequencing. Informative explanations of why certain steps are necessary and extensive tips that help make the difference between success and failure are also included. This book will offer time-saving techniques for even highly skilled researchers.
Z364452 PCR Strategies A compilation of short articles written by PCR experts specifically for this volume. Practical information and advice includes background on key PCR concepts, analysis of PCR products, special applications, and alternatives to PCR for gene amplification.
P5855 PCR Technique: Quantitative PCR This volume in the “BioTechniques Update Series” collects a number of important papers focused on various aspects of quantitative PCR that first appeared in BioTechniques from 1991 to 1996. This period of time witnessed the introduction, rapid development and numerous improvements of this laboratory method. These papers, most of which were reviewed and updated by the original authors, present novel methods to quantitate PCR products, improvements and modifications of previously published methods, and applications of quantitative PCR to several clinical situationa.
Z704318 PCR Technology: Current Innovations, 2nd ed. This book is a fundamental reference for scientists new to PCR technology and a source of up-to-date applications for those familiar with the method. It provides theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. The book begins with discussions of the preparation of PCR experiments, followed by examples of analytical PCR divided into qualitative and quantitative applications. The final section explores preparative methods addressing DNA generation for further analysis and in vitro evolution. Featuring detailed protocols, this volume contains critical information for practitioners in a wide variety of fields, including forensics, molecular biology research, clinical DNA diagnostics, botany, and paleontology.
Z705489 PCR Troubleshooting: the Essential Guide A unique PCR troubleshooting guide that is an essential companion for anyone who uses the polymerase chain reaction technique. Aimed at a reader with some experience in PCR the book discusses the many and varied problems encountered with PCR together with tips, advice and procedures to obviate rather than overcome the PCR problems. Written in the language of the laboratory with a little humour and a down-to-earth approach, the book is easy to read and understand. If you fail at PCR, consult this book! The advice in these pages is invaluable and is the sort of advice that is not usually found elsewhere.
Z379603 PCR in Bioanalysis: Methods in Molecular Biology, Vol. 92 Marking the transformation of PCR from a purely research technique to an analytical tool, this volume details 22 cutting-edge protocols for molecular diagnosis of specific diseases. Coverage extends to infectious diseases, genetic syndromes, and cancer in humans, animals and plants. Diagnose such diseases as hepatitis C, tuberculosis, chlamydia and trichomonad infections. Animal tests include those for a bovine HIV-like virus, rodent parvoviruses, and Ross River virus. There are also general protocols for selective UV radiation fractionation (SURF) and for detecting circulating cancer cells. Overall, a concise but thorough introduction to practical diagnostic PCR.
Z702374 PCR/RT-PCR in situ Light and Electron Microscopy Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. This book covers methods of in situ PCR and reverse transcription PCR (RT-PCR), two approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. It provides theoretical consideration, as well as practical approaches to in situ PCR. Authors provide detailed protocols including the preparation of tissue samples, the rationale for the design of primers and revelation. They emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity.
P7186 PCR: A Practical Approach This book covers gene analysis at the level of restriction fragment polymorphism, point mutation, and sequence analysis. Methods are given for using different source materials: cloned DNA, genomic DNA, RNA, nucleic acid from archive material, and PCR products themselves.
Z704857 PG200 (Progenesis) software Progenesis PG200 provides simple laboratory image analysis for single stain 2D gels using a routine, three step approach – Set-up, Process and Review. With this approach an analysis is set-up in minutes, processed automatically and reviewed using a comprehensive suite of display options to show spots of interest. Analysis workflows can be tailored to the specific requirements of your lab using an intuitive user interface and step by step approach. Key features of PG200 include
• Analysis Wizard to set-up entire an experiment for analysis
• Automated Matching with option for manual seed placement
• Background subtraction that is flexible and fully informative
• Normalisation methods to perform accurate intensity calibration and MW/pl determination on your 2D gels
• Editing tools to optimize analysis results
• SameSpots spot outline method, which offers a brand new approach for analysing aligned images
• Image views and measurements tables that highlight areas of interest and present results
• Workspaces that can be recalled to display custom window layouts for easy data review
Z705608 PRINS and In Situ PCR Protocols, 2nd ed. This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection.
Z373931 PRINS and In Situ PCR Protocols: Methods in Molecular Biology, Vol. 71 This new, cutting edge publication demonstrates clearly how oligonucleotide primers may be successfully used to detect and amplify, or extend, complementary sequences ::FIin situ. It provides methods for rapid identification and characterization of chromosomal cDNA sequences, viral genomes, and rare messenger RNAs in cells.
Z276642 Palladium Reagents and Catalysts: Innovations in Organic Synthesis Palladium is one of the most popular compounds used in the synthesis of pharmaceuticals, foods, cosmetics, and materials worldwide. Now in paperback, this book provides a comprehensive overview of palladium chemistry, highlighting recent discoveries and applications, and detailing new synthetic methods.
Z706027 Palladium Reagents and Catalysts: New Perspectives for the 21st Century Jiro Tsuji, one of the pioneers in this field of organic synthesis, provides synthetic organic chemists with a remarkable overview of the many applications of organopalladium chemistry. Tsuji discusses the recent developments in the field as well as the explosive growth over the last five years. Highlighting the most recent discoveries in this rapidly expanding field, the book focuses on new aspects of organopalladium chemistry, putting emphasis on synthetic applications and investigates the new perspectives on the synthetic uses of contemporary organopalladium chemistry This is an essential reference source and companion for students, and both industrial and academic research chemists working in organic synthesis, particularly on synthesis of natural products and medicinal compounds.
Z730203 Palladium in Heterocyclic Chemistry: A Guide for the Synthetic Chemist, 2nd ed. Heterocycles are of paramount importance in the pharmaceutical industry and palladium chemistry is one of the most novel and efficient ways of making heterocycles. Today, palladium-catalyzed coupling is the method of choice for the synthesis of a wide range of biaryls and heterobiaryls. The number of applications of palladium chemistry to the syntheses of heterocycles has grown exponentially. These developments highlight the need for a monograph dedicated solely to the palladium chemistry in heterocycles and this book provides a comprehensive explanation of the subject. The principal aim of the book is to highlight important palladium-mediated reactions of heterocycles with emphasis on the unique characteristics of individual heterocycles. This book comprises a compilation of important preparations of heteroaryl halides, boranes and stannanes for each heterocycle.
P9234 Passion for DNA: Genes, Genomes, and Society A principal architect and visionary of the new biology, a Nobel Prize-winner at 34 and best-selling author at 40 (The Double Helix), James D. Watson had the authority and courage to take a prominent role as commentator on the march of DNA science and its implications for society. In essays for publications and in lectures, he delivered what were, in effect, dispatches from the front lines of the revolution. This book is a collection of outspoken and sparkling ideas and opinions.
Z700657 Patch-Clamp Analysis: Advanced Techniques This book describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, it presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Z528684 Patent Strategy for Researchers and Research Managers, 2nd ed. This edition introduces researchers to patent applications and patent portfolios. With minimum use of ''legal jargon", it provides the technical professional with the help and advice they require to understand the legal complexities that they may encounter before and during a patent application. It also discusses the responsibilities of the researcher after patent applications have been filed and the role the researcher can play in the maintenance of a global patent estate. Over the last five years intellectual property has taken center stage around the world as countries realize the value of having a consistent and enforceable patent business. The increase in patent activity has resulted in a number of new laws being created. To reflect these changes this edition has been reorganised and completely updated.
Z702811 Patents, Copyrights & Trademarks for Dummies This book explains, in layman's terms, the basic nature, function, and application of intellectual property (IP) rights, including how you can acquire those rights, wield them effectively against your competitors, or exploit them lucratively through licensing agreements and other rewarding adventures. This book covers all of these critical concepts, such as Working with IP professionals, Presenting a patent explanation, Determining what is copyrighted and what isn't, Protecting your commercial identity, Inspecting the basic elements of a license, Determining infringement, Avoiding the ten worst naming blunders.
P1994 Peptide Antibiotics: Discovery, Modes of Action and Applications This reference summarizes the latest research on the structure, function, and design of synthetic and natural peptide antibiotics, describing practical applications in food preservation and packaging, and in the prevention and treatment of infectious diseases by direct antibacterial action and as part of the adaptive immune response. It discusses these compounds and their applications including: the distribution and classification of diverse, antimicrobial peptides throughout nature, the role in host defense of mucosal surface peptide antibiotics such as defensins and cathepsins, and the genetic basis determining the production of bacterial peptide antibiotics.
Z700215 Peptide Arrays on Membrane Supports: Synthesis and Applications Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented.
Z703826 Peptide Synthesis and Applications Experts describe in detail the methodologies of contemporary peptide synthesis and illustrate their numerous applications. Techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Z553751 Peptides: Biology and Chemistry Peptides play a decisive role in many physiological processes, whether as neurotransmitters, hormones or antibiotics. The rapid developments in peptide research over the past few decades make it almost impossible for newcomers to gain an overview. This unique work covers all the important aspects of this wide-ranging field in one handy volume. On the basis of the fundamental chemical and structural properties of peptides, this reference runs the gamut from analysis, the occurrence and biological importance of peptides, via chemical, biochemical and genetic methods of peptide synthesis, right up to peptide libraries, peptide design and their role in drug research. Yet this book offers much more than a mere overview of the latest level of research. An encyclopedic appendix with valuable data on more than 500 biological relevant peptides and proteins, a comprehensive register and details of further literature references make this the ideal reference for all questions regarding peptide research.
Z410217 Perfume Handbook, 2nd ed. The first edition of this book established itself as an unparalleled source of information on perfume. This edition is now aimed squarely at perfumery marketing specialists and others in the industry and covers in particular the needs of publicity/advertising teams, who like to have a wide range of information at their fingertips. Changes include: an expansion of the number of profiles of the perfume houses, and of the 50 or so new perfumes worthy of record which have been launched since the previous edition. There is also increased coverage of the essences and the plants and other material from which they are derived. Coverage of perfume containers is substantially expanded and linked to other parts of the book
Z704121 Perspectives in Flavor and Fragrance Research Research is more than ever central to the F&F industry with its constant demand for innovation and its frequently changing trends. Especially, in the classic and well-explored domains of musks and amber odorants fascinating new discoveries were made only very recently, which proves the endless possibilities in the search for new aroma chemicals. Fragrance and fragrance materials by definition elicit a biological response, serve as versatile signals, trigger the sense of smell and taste in various ways - and every odorant design is nothing more than 'chemistry probing nature'. But Fragrance Chemistry can also document and even preserve the biodiversity of scents.
Z701939 Pesticide Manual, 13th ed. This edition of this world compendium is an essential reference book for anyone with a professional interest in pesticides. Extensively revised and updated, it contains 858 detailed main entries as well as abbreviated details covering 611 superseded products. Entries cover herbicides, fungicides, insecticides, acaricides, nematicides, plant growth regulators, herbicide safeners, repellents, synergists, pheromones, beneficial microbial and invertebrate agents, rodenticides and animal ectoparasiticides. 50 new entries added, including more than 30 new synthetic molecules, 13 further minor substances not previously listed and several more pheromones
P5861 Phage Display: A Laboratory Manual Phage display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage display technology is challenging and the aim of this manual is to provide comprehensive instruction in its theory and application.
Z730815 Phage and the Origins of Molecular Biology, the Centennial Edition This hugely influential book, published in 1966 as a 60th birthday tribute to Max Delbrück, is now republished as The Centennial Edition. On first publication, the book was hailed as “[introducing] into the literature of science, for the first time, a self–conscious historical element in which the participants in scientific discovery engage in writing their own chronicle. As such, it is an important document in the history of biology...” (Journal of History of Biology). And in another review it was described as “required reading for every student of experimental biology...[who] will sense the smell and rattle of the laboratory” (Bioscience). The book was a formative influence on many of today’s leading scientists.
Z701637 Pharmaceutical Analysis This single-volume text explores the analytical aspects of drug development and manufacture. This reference provides pharmacists and scientists - specifically, analytical and organic chemists and biochemists -- with a source of information to a broad range of techniques and their applications. This allows readers to choose the most appropriate analytical technique for a particular purpose. The text discusses a variety of concepts, including: quality control and regulatory requirements, chiral and solid state analyses, vibrational spectroscopy, particle properties, microscopy and imaging, and nuclear magnetic resonance
Z700746 Pharmaceutical Chemistry: Therapeutic Aspects of Biomacromolecules In this introductory book, key strategies involved in the development and production of a range of clinically useful compounds are presented along with a broad overview of the way in which peptides, proteins, nucleic acids and carbohydrates are used therapeutically. Illustrated with numerous examples, the book covers essential compounds and explains how they exert their influence through an understanding of biological processes. Pharmaceutical chemistry has become progressively more important in terms of both research and teaching, and this book includes latest developments with examples selected in order to emphasise the molecular basis of specific disease processes
Z703206 Pharmaceutical Excipients: Characterization by IR, Raman and NMR Spectroscopy Recognizing the need for a hands-on guide elucidating the role of molecular spectroscopy in the physical characterization of pharmaceutical solids, two experts gather theoretical discussions of infrared, Raman, and NMR spectroscopy, and helpful recommendations on spectral data acquisition techniques, as well as 600 spectra for 300 of the most commonly used excipients in pharmaceutical dosage formulation. Furnishes accurate spectral data on each compound for interpretation, such as peak listings for identifying observed spectral features, excipient grades, Chemical Abstracts Service (CAS) registry numbers, molecular formulae, molecular weights, other common excipient names, and excipient usage.
Z515078 Pharmaceutical Master Validation Plan: The Ultimate Guide to FDA, GMP, and GLP Compliance Pharmaceutical Master Validation Plan: The Ultimate Guide to FDA, GMP, and GLP Compliance will allow you to more easily achieve satisfactory inspections, new medical product approval, minimize non-conformance, reduce rework and rejected lots, and avoid recall lots by developing and managing a Master Validation Plan. The accompanying CD allows users to input the template plan into their computers and tailor it to incorporate additional regulatory requirements specific to individual companies worldwide and print the required documents. Together, the book and CD contain everything required to develop and execute a successful Master Validation Plan based on FDA guidelines for the pharmaceutical industry, and allows the templates to be extended to diagnostic products, medical device, medical equipment, and biotech industry products. It supplies valuable text establishing a master plan in compliance with FDA, GMP, and GLP requirements and provides a missing link between the Validation Master Plan to the Validation Standard Operating Procedures and Protocols developed and executed
The Master Validation Plan provides a roadmap to management for on-time start-up of facility operations, and validation of existing facilities, in compliance with GMP requirements. The lack of a comprehensive Master Validation Plan and well-documented validation procedures is the main reason that new drug, medical device, medical equipment, and related product applications are rejected by the FDA. This thorough guide provides the needed solutions and guidance for both foreign and U.S. companies to achieve FDA compliance and authorization to market their products in the United States.