Life Science

New Books

Reset Table

Image	Product #	Book Title	Description
	Z730580	Animal Cell Biotechnology: Methods and Protocols, 2nd ed.	This second edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques.
	Z731005	Biochemistry 6th ed.	In the new edition of Biochemistry, instructors will see the all the hallmark features that made this a consistent bestseller for the undergraduate biochemistry course: exceptional clarity and concision, a more biological focus, cutting-edge content, and an elegant, uncluttered design. Accomplished in both the classroom and the laboratory, coauthors Jeremy Berg and John Tymoczko draw on the field’s dynamic research to illustrate its fundamental ideas.
	Z730998	Cellular and Molecular Immunology 6th ed.	An exceptionally lucid guide to the latest immunology concepts! Through five best-selling editions, readers worldwide have appreciated this book's in-depth yet straightforward, richly illustrated approach to this complex subject. Now, sweeping updates incorporate the latest discoveries about innate immunity, the organization of lymphoid organs, and many other cutting-edge topics. Plus, online access via STUDENT CONSULT makes this an even more powerful learning resource!
	Z730777	Genetic Variation: A Laboratory Manual (pb) Paperback	This manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. It covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. The early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.
	Z730718	In Vitro Transcription and Translation Protocols 2nd ed.	This Second Edition provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration and of continuous-exchange devices to obtain high levels of in vitro protein production, the efficient in vitro production of integral membrane proteins, a protein category known to be extremely resistant to in vivo expression, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. Other chapters also discuss the efficient, high-throughput protein production using the wheat germ in vitro expression system and exogenous protein expression in xenopus oocytes. Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy and to follow the surge of drug resistance, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the use of in vitro transcription and translation to directly identify proteins in 2D-maps. Additional chapters investigate high-throughput in vitro expression of Bacillus anthracis antigens to identify vaccine candidates, the use of Xenopus laevis translation system to study the functional expression of type 1 rat GABA transporter, and the exploitation of wheat germ in vitro transcription and translation system for the production of highly purified proteins amenable to NMR studies.
	Z730971	Merck Manual 18th ed.	The 18th Edition features a wealth of new content and greater ease of use. • New clinical approach chapters • New disease summaries • 34 new chapters and 69 new illustrations • New cross-referencing to specific pages • New two-color printing • Written by more than 300 medical experts in various fields of medicine • All text has been peer reviewed
	Z731110	PCR Primer Design	In this book authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome scale experiments and for small scale individual PCR amplifications. This volume will be useful for organizations perfomring whole genome studies, companies designing instruments that utilize PCR, and individual scientists who routinely use PCR in their research.
	Z730696	Quantitative Proteomics by Mass Spectrometry	This book is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers. Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS. .
	Z730734	Regulatory RNAs Paperback	Regulatory RNAs” was the theme of the 71st annual Cold Spring Harbor Symposium on Quantitative Biology, where scientists from around the world presented the latest advances in the biology of RNAs, including RNA interference, transcriptional and translational control, RNA editing, and the role of RNAs in biological circuits and epigenetic events. Investigators discussed the latest technologies aimed at large-scale characterization of RNAs, as well as the roles of small RNAs in development and cancer. This volume is a timely survey of this important new area of molecular biology.

Copyrights © 2008 Sigma-Aldrich Co. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc.