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Z703028 B Cell Protocols Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. This book is a diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods in B cell biology. These readily reproducible protocols follow the successful Methods in Molecular Biology series format.
B7157 Baculovirus Expression Vectors: A Laboratory Manual This laboratory manual simplifies the selection of the most appropriate vector design for a given problem and describes each step in the implementation process from vector construction to large-scale protein production.
B7058 Bailey & Scott’s Diagnostic Microbiology, 11th ed. The classic text known as the 'gold standard' in microbiology is now revised, reorganized, and up-to-date. Always comprehensive and current, this edition features even more new information on hot topics such as identification systems, quality control organisms, antiparasitic agents, HIV viral load testing, HIV genotyping, Hepatitis C virus, antivirals, and a new procedure for the motility test. The additional and revised material reflects the latest advances and developments. New clinical case studies challenge students to think critically and apply what they've learned in realistic situations.
Z702919 Basic Cell Culture Protocols 3rd ed. This third edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research
B2183 Basic Cell Culture: A Practical Approach, 2nd ed. This book is a guide to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced and novice researchers should find this book useful as a source of reference, when entering a new field, or when setting up a new laboratory.
500003 Basic Gas Chromatography This book is a product of three basic gas chromatographic courses that Varian Aerograph offers throughout the world. Useful data are presented, including calibration factors for detectors, chemical structures of liquid phases, and methods for preparing liquid phases and packing columns. The authors hope readers will confirm their belief that a major advantage of GC is its simplicity.
Z406406 Basic Gas Chromatography: Techniques in Analytical Chemistry From Book News, Inc.
Offers thorough coverage of basic principles and techniques of modern gas chromotography, serving as a primer/working reference for bench chemists and as a text for upper-level undergraduate and graduate students. Theoretical issues are explained without complicated equations and derivations and always in terms of how they relate to practical operating principles. Coverage includes both capillary column and packed column chromotography, as well as cutting-edge topics such as chiral analysis and solid phase microextraction. Includes step-by-step guidelines and trouble shooting tips, reference appendices, and use of new IUPAC terms cross-referenced to traditional terms and symbols. Book News, Inc.®, Portland, OR
B4931 Basic Methods in Antibody Production and Characterization This manual uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described include polyclonal and monoclonal and those made by the phage display. It focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications. Ample references provide researchers with many applications pertaining to the technology.
Z719102 Basic Methods in Microscopy Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. This book provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events.
B5678 Basic Molecular Biology: Essential Techniques Begins with a brief overview and then focuses on simple step-by-step protocols of the core techniques. It provides not only a theoretical outline, but also valuable advice on real-world problems and estimates on how much time is required to perform each technique.
Z703311 Basic One- and Two-Dimensional NMR Spectroscopy, 4th Completely revised edition A classic among NMR textbooks, this thoroughly enlarged and updated fourth edition includes polymer solid state NMR, analysis of biopolymers, and applications of Magnetic Resonance Tomography and Magnetic Resonance Spectroscopy. Written by an NMR expert with long-standing teaching experience, this text provides students and professionals with a lucidly written introduction to NMR spectroscopy. The book forms an excellent bridge between the very simple texts on spectral interpreataion and more specialist works with an emphasis on mathematical theory.
Z704903 Beacon Designer Beacon Designer helps design successful SYBR™ Green, TaqMan™, LNA spiked TaqMan, molecular beacon for both standard and NASBA assays and FRET assays by interpreting BLAST results for specificity and locating oligos avoiding template structures.
SYBR Green Primer Design- Highly specific primers are designed by avoiding significant homologies found by automatically interpreting BLAST search results. Primer efficiency is enhanced by avoiding template secondary structures identified by connecting to the Mfold server.
Dual Labeled Probe Design: Dual-labeled fluorescent TaqMan, molecular beacon or FRET probes can be designed with the help of Beacon Designer. For these assays, you can design new primers and probes or find probes compatible with previously designed SYBR Green assays and save on synthesis costs.
TaqMan probes and Molecular Beacon Multiplex experiments- Multiplex assays are designed by avoiding homologies with all primers and probes in the pool. This prevents competition in multiplex real time PCR reactions and assures high signal strength.
Allelic Discrimination- Design both wild and mutant probes for SNP genotyping assays.
LNA Probe Design- In addition to standard TaqMan probes, Beacon Designer also designs the increasingly popular LNA or Locked Nucleic Acid substituted TaqMan probes to improve real-time PCR assay success.
NASBA Assays- Beacon Designer designs molecular beacon probes for NASBA assays for single template, multiplex or alleleic discrimination.
BLAST Search- Both primers and templates/amplicons can be BLAST searched against genomic databases available at NCBI or against local custom databases. This function helps verify the primer specificity.
Avoid Template Structures- Automatically avoid secondary structures in the template identified by connecting to the Mfold server while designing primers.
Generate Report- Generate an attractively formatted
B3184 Before & Beyond Patents This guide provides answers to frequently asked questions on topics such as - Becoming an inventor; Managing inventions and discoveries - step-by-step; Inventions - Rights of collaborators and young scientists; Rights of co-inventors; Effects of disclosing research results before protecting patent rights; How sharing and transfer of biological materials affects patent rights; Inventions and how to go about setting up a business.
Z700843 Beginner’s Guide to Microarrays The manufacture, experimental design, and analysis of microarrays are not always straightforward, and researchers run into roadblocks that hinder progress. This book is directed to the core facility manager who produces, hybridizes, and scans arrays, and the research scientist who will be performing the analysis and interpreting the results. It provides a user friendly coverage and protocols for the technical steps and procedures involved in many facets of microarray technology, including: Coating glass slides; Designing oligonucleotide probes; Troubleshooting spotting problems; Normalizing background signal and controlling for systematic variance; Analyzing data with statistical procedures.
B0411 Bergey’s Manual of Determinative Bacteriology, 9th ed. Based on the data contained in the four-volume Bergey's Manual of Systematic Bacteriology, this book also includes new genera and species, new combinations, and new taxa published through the January 1992 issue of the IJSB. Users will find short general descriptions that encompass all organisms by Groups; shape and size, Gram reaction, other pertinent morphological features, motility and flagella, relations to oxygen, basic type of metabolism, carbon and energy sources, habitat and ecology. BMDB-9 also includes discussions of difficulties in identification, keys or tables to genera and species, genus descriptions, synonyms, other nomenclatural changes, and numerous illustrations.
B8305 Beyond Neurotransmission This book provides the foundations for understanding the cellular and molecular basis for neuromodulatory effects. It illustrates key examples of the roles played by neuromodulation in sensory processing, neuromuscular transmission, generation of motor behaviors, and learning. The book also underscores the importance of information processing by the nervous system in future studies. By spanning the levels of analysis from sub-cellular mechanisms through cellular properties and neuronal systems to behavior, the book provides a framework for understanding this field.
Z730084 Binding and Kinetics for Molecular Biologists This handbook offers a practical guide to the principles of quantitative analysis in biological experiments. The material is primarily aimed at working molecular biologists, but the scope and clarity of presentation make it equally suitable for students. Topics covered range from the basics—such as measuring the concentrations of macromolecules—through considerations of binding constants and the kinetics of molecular interactions. The book ends with a thorough consideration of data analysis.
Z702625 Bio conjugation Protocols: Strategies and Methods Experts update the classic bioconjugation methods and introduce techniques that go beyond pure biomedical applications to include elements from organic synthesis, molecular biology, and materials science. These methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address on-chip synthesis to generate microarrays for the high-throughput analysis of RNA and DNA.
Z700541 BioNMR in Drug Research This book presents the theoretical background on NMR of biomolecules, plus the use of NMR techniques in determining the structures of proteins and nucleic acids. BioNMR spectroscopy offers a universal tool for examining the binding of an active substance to its target protein. Its use benefits the rational development of drugs. This interaction can now be investigated and displayed in 3D - an important prerequisite for the targeted development of new active substances. The latest methods for characterizing substance-receptor complexes are demonstrated backed by case studies from pharmaceutical research. This guide contains basic information, application strategies and real-life examples.
Z702951 Bioanalytical Chemistry Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples. This book covers: Spectroscopic methods for matrix characterization; Structural and functional properties of antibodies; Principles of electrophoresis; Centrifugation methods; Mass spectrometry of biomolecules; Quantitation of enzymes and their substrates; Design and implementation of enzyme assays; Isoelectric focussing; Chromatography of biomolecules; and Validation of new bioanalytical methods.
Z704768 Biocatalysts and Enzyme Technology A comprehensive overview of current knowledge of biocatalysis and enzyme technology. Following an introduction to the history of enzyme applications and the motivations for using these highly selective and environmentally friendly methods, the book goes on to cover enzyme mechanisms and kinetics, production, recovery, characterization and their design, including recombinant methods. Alongside the application of soluble and immobilized biocatalysts, including whole-cell systems, the authors treat the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. In line with the book's didactic approach, a number of case studies further exemplify the advantages of enzyme processes. Each topic includes exercises, designed to facilitate access to this area of research.
Z510734 Biocatalysts for Fine Chemicals Synthesis Includes a state-of-the-art review on biocatalysis followed by a collection of protocols covering areas where cells and enzymes have been proven to be useful catalysts. Contains fully tested and validated protocols and Step-by-step instructions for the expert and the inexperienced chemist
B9911 Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology This truly unique road map to modern biochemistry is an atlas of multi-step reactions in biological systems. The book graphically represents this network of reactions with a series of chemical formulas along with two-dimensional spatial arrangements demonstrating each molecule's cellular, organic, and structural aspects. It is an encyclopedic, comprehensive, and clearly organized reference for any biochemist.
Z704644 Biochemistry and Molecular Biology 3rd ed. An understanding of biochemistry and molecular cell biology opens the door to an understanding of life itself. Biochemistry and Molecular Biology introduces this subject to the widest range of students, providing insights into this fascinating area. The new edition takes students from the fundamentals of cell biology and biochemistry to the latest advances in genomics and proteomics. Maintaining the clear, accessible approach of previous editions, this volume is enriched by carefully layered explanations and vivid illustrations throughout.
B6308 Biochemistry of Cell Signalling This will serve as a reliable map through the maze of cell signaling pathways and help the reader understand how malfunctions in these pathways can lead to disease. Part 1 describes the machinery of signal transduction starting with the properties of signals, receptors (including receptor activation), regulators, and the molecules that link receptor and regulator. Part 2 describes the implementation of the signaling cascades focusing on the effect on gene transcription. Part 3 studies the global cellular regulatory programs for the control of cell growth and proliferation. Part 4 considers loss of regulatory control and its consequences with respect to the molecular basis of cancer.
Z701297 Biochemistry of Signal Transduction and Regulation, 3rd ed. This book has become the "gold standard" in the field of signal transduction and offers unmatched quality from the pen of a single author. The author succeeds in balancing comprehensive coverage with a clear and didactic style. The new edition is completely updated. 50% of the content has been rewritten to incorporate recent findings, and many illustrations have been redrawn and are now presented in full color.
Z700444 Biochemistry:The Chemical Reactions of Living Cells, 2nd ed., Vol. 1 The most comprehensive textbook/reference ever to cover the chemical basis of life, the "Green Bible of Biochemistry" has been a well-respected contribution to the field for more than twenty years. The complex structures that make up cells are described in detail, along with the forces that hold them together, and the chemical reactions that allow for recognition, signaling and movement. There is ample information on the human body, its genome, and the action of muscles, eyes, and the brain. The complete set deals with the natural world, treating the metabolism of bacteria, toxins, antibiotics, specialized compounds made by plants, photosynthesis, luminescence of fireflies, among many other topics.
Z703559 Biocomputing: Computer Tools For Biologists BioComputing: Computer Tools for Biologists contains a selection of BioTechniques' best Biocomputing papers from 1996-2002 augmented with original author updates. This comprehensive collection provides researchers with simple programs and open source code that can be used as models to study, copy, and modify for developing new computer programs. It also contains useful tools and insights into how to design better queries and interpret results more carefully, thus making more effective use of websites such as BLAST and PubMed.
Z374210 Bioconjugate Techniques A practical guide to modification and cross-linking technology for research, diagnostics and therapeutics. Contains detailed information on the chemistry, reagent systems and practical applications for creating labeled or conjugated molecules.
Z269662 Biodegradable Hydrogels for Drug Delivery This book provides an in-depth investigation of recent advances in biodegradable hydrogels. Based on extensive research and experience, the book is the first to deal exclusively with this important area in drug delivery. A valuable guide for all, the book begins with a detailed introduction to current biodegradable hydrogel technology. Following chapters present advanced R&D, delivery systems, and more. It covers the mechanisms of biodegradation, types of biodegradable hydrogels, chemical and physical gels, chemical and enzymatic degradation, and examples of biodegradable drug delivery systems.

Z705004 Biofunctionalization of Nanomaterials Nanotechnologies for the Life Sciences (NtLS) is the first comprehensive source covering the convergence of materials and life sciences on the nanoscale. Tailor-made nanomaterials find widespread new opportunities in diagnostic and monitoring microdevices, microsurgery tools and instruments, tissue engineering, drug delivery artificial organs and any more. NtLS is essential reading for all scientists working in this field from medicine and biology through chemistry, materials science and physics to engineering. Volume 1 brings together the synthetic methods and materials aspects of instilling biocompatibility and desirable properties into nanomaterials, covering numerous types and methods.
B5056 Bioinformatics Basics: Applications in Biological Science and Medicine Scientists use an extensive databank to access structural information on proteins and nucleic acids and a specialized form of software to analyze the hybridization pattern of DNA chips. The success of biotechnology and pharmaceutical industries has been a result of technical advancements in genomics, proteomics, and bioinformatics. This book describes the organization of public domain databases with a tutorial for the services described. It concentrates on three major database clusters and relevant software tools that are maintained in the US, Europe, and Japan.
Z704415 Bioinformatics and Drug Discovery A collection of readily reproducible bioinformatic methods to advance the drug discovery process from gene identification to protein modeling to the identification of specific drug candidates. The authors demonstrate these techniques, including microarray analysis, the analysis of genes as potential drug targets, virtual screening and in silico protein design, and cheminformatics, in a variety of practical situations. Because these technologies are still emergent, each chapter contains an extended introduction that explains the theory and application of the technology and techniques described.
Z701718 Bioinformatics and Functional Genomics Encompassing the field of bioinformatics, from sequence analysis, gene expression, microarrays, and proteomics to molecular phylogenics and whole genome studies, this text shows how bioinformatics applies to biological questions at different levels, such as gene/protein, disease, and protein structures. The author emphasizes practical skills, such as how to analyze genes and proteins, how to make trees using phylogenic software, how to extract data, and how to identify genes and proteins implicated in disease. It provides a systematic three-part format, beginning with the analysis of biological sequence data, continuing with functional genomics (from microarrays to proteomics), and ending with coverage of genomes.
Z730386 Bioinformatics for Dummies 2nd ed. Explore the human genome and analyze DNA — without leaving your desktop! All it takes is the basics of molecular biology and the wealth of information in this friendly guide. You'll discover the online tools and useful tips you need to ask the right questions, analyze sequences, and compare results. It's a plain-English introduction to the field! Discover how to do biological research online; Use gene-centric databases; Visualize proteins in 3-D; Interpret and analyze sequences; Produce and publish results.
Z703591 Bioinformatics: A Guide to the Analysis of Genes and Proteins, 3rd ed. Using relevant biological examples, the book provides background on and strategies for using many of the most powerful and commonly used computational approaches for biological discovery. This new edition features new chapters on genomic databases, predictive methods using RNA sequences, sequence polymorphisms, protein structure prediction, intermolecular interactions, and proteomic approaches for protein identification. It contains detailed worked examples illustrating the use of the concepts presented. Special topic boxes and appendices highlighting experimental strategies and advanced concepts are included with annotated reference lists, comprehensive lists of relevant Web resources, and an extensive glossary of commonly used terms in bioinformatics, genomics, and proteomics
B2931 Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, 2nd ed. The second edition covers the broad spectrum of topics in bioinformatics, ranging from Internet concepts to predictive algorithms used on sequence, structure, and expression data. It includes: (a) all new end-of-chapter Web resources, bibliographies, and problem sets, (b) accompanying Website containing the answers to the problems, as well as links to relevant Web resources, (c) new coverage of comparative genomics, large-scale genome analysis, sequence assembly, and expressed sequence tags, (d) a glossary of commonly used terms in bioinformatics and genomics.
B8308 Bioinformatics: From Genomes to Drugs, 2 Volume Set Bioinformatics promises to revolutionize the process of drug discovery and development. This book provides an application-oriented overview of this technology. In addition, the state-of-the-art in bioinformatics is evaluated from a global view by introducing real application scenarios such as genome projects that require the use of a whole set of bioinformatics tools. The knowledge on bioinformatics presented enables readers to go beyond a mere push-button approach to using bioinformatics software and interpreting the data generated, but also to assess the potential and limitations of today's bioinformatics software and future challenges.