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Z402338 HPLC Columns: Theory, Technology, and Practice High performance liquid chromatography (HPLC) is the most common analytical method for separating substances into their individual components. As the separations occur in a column, this part of the HPLC apparatus is crucial to achieving the best results. This state-of-the-art overview of HPLC column technology covers fundamental principles, column selection criteria and special techniques.
Z730149 HPLC Made to Measure: A Practical Handbook for Optimization The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.
Z424382 HPLC Methods for Pharmaceutical Analysis 4-Volume Set Provides fast, hands-on access to thousands of abstracts from the current literature on HPLC methods for pharmaceutical analysis.
The most commonly used method for analyzing substances, and the first method most researchers turn to, is high performance liquid chromatography (HPLC). Following up on a best-seller, volumes 1-4 continue to provide an easily-accessible collection of procedures for analyzing pharmaceuticals using HPLC.

Z704296 HPLC Methods for Recently Approved Pharmaceuticals Stop wasting time hunting for the right HPLC assay techniques. With this book, you will quickly identify and replicate the ideal procedures without having to refer to original source publications. It arms you with established HPLC assay techniques for hundreds of newly approved drugs, as well as drugs for which assay methods were only recently developed. This handbook includes: HPLC methods for 390 commonly prescribed pharmaceutical compounds; Methods for drugs in biological fluids and for bulk and formulated drugs; Chemical structures, molecular weights and formulas, and CAS Registry Numbers; Cross-references to The Merck Index; Retention times of other drugs that can be assayed using the same methods.
Z514233 HPLC Solvent Guide, 2nd Ed. Solvent selection is perhaps the most commonly overlooked parameter in HPLC. Even the most experienced analytical chemist tends to select one of three familiar solvents. The HPLC Solvent Guide provides detailed coverage of all commonly used HPLC solvents used in a wide range of separations. This book give a practical overview of the role of solvents in HPLC method development. Details the physical, chemical, and chromatographic properties of the solvents followed with specific analytes.
Z730246 HPLC for Pharmaceutical Scientists HPLC for Pharmaceutical Scientists is an excellent book for pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.
Z702269 HPLC of Biological Macromolecules, 2nd ed. Revised and expanded to reflect recent innovations in HPLC, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides and offers class-specific applications for the characterization and fractionation of biological macromolecules. With contributions from experts, this edition provides new chapters summarizing specialized detection systems; current discussions on the chemical and biological properties of specific biomolecules; detailed guidelines for the development of modern analytical techniques; state-of-the-art exploration methods for complex mixtures; successful implementation and investigation procedures.
H3409 HPLC of Macromolecules High pressure (or performance) liquid chromatography is a standard biochemical technique used for the separation of molecules. This volume covers the larger biomolecules - oligosaccharides, glycopeptides, oligonucleotides, polypeptides, and proteins. It also includes the latest advances in microbore and packed capillary technology and the use of mass spectrometric detection.
Z701890 HPLC of Peptides and Proteins: Methods and Protocols Hands-on experts from academia and industry describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology
Z525391 HPLC: Practical and Industry Applications, 2nd ed. Since the publication of the first edition, the areas of mass spectroscopy, electrophoretic separations, and ultra-micro separations have blossomed. The Second Edition addresses all of these changes and more. The book is fully current, examining analytical HPLC as it is actually used in industry.
H6033 Haematopoietic and Lymphoid Cell Culture This reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact haematopoietic and lymphoid cells, and their maintenance as primary cultures. It provides technical information on the signals and mediators required for the differentiation and growth of these cells. Chapters discuss dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, hematopoietic differentiation of embryonal stem cells, and culturing of murine thymic explants.
Z261777 Handbook Of Organic Solvents Presents important information about more than 550 organic compounds that are used as solvents. Includes name, synonyms, molecular formula, CAS Registry Numbers, and useful data for all of the compounds. Health hazards and safety guidelines also discussed.
Z703087 Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology ATP-dependent active ion transport enables cells to regulate their pH value and to control their ion composition. The reverse process, transforming an ion imbalance into chemical energy, drives mitochondrial and chloroplast ATP synthesis. The mediators of these fundamental processes are ion-motive ATPases, highly conserved enzymes that play key roles in cell physiology from bacteria to man. This handbook summarizes recent knowledge about the molecular mechanism of ATPases, relating this information to the physiology and pathophysiology of ion transport, mitochondrial function, vesicle transport and lysosomal acidification. All important P-type, F-type and V-type ATPases are treated systematically, complemented by a special section on the cell biology and physiology of acidic compartments.
H1897 Handbook of Analytical Derivatization Reactions Much of the analytical work involving GC, MS, GC-MS and more recently HPLC involve analytical derivatization prior to analysis. This book presents a collection of these derivatization methods. Those described here involve covalent derivatives of organic compounds.
Z705365 Handbook of Assay Development in Drug Discovery The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery. This book is a reference that describes the complete armament of tools available for performing various assay techniques. It presents descriptions of methods, laboratory guidelines and protocols used to perform such methods, specific examples of each assay system, and troubleshooting tools. The handbook describes biochemical assay classes as well as non-class specific assay development for cell-based assays. It covers a wide range of target classes-including kinases, proteases, nuclear receptors, and GPCRs-and describes currently employed methods and assay types, such as radioligand binding assays, image analysis assays, enzyme fragment complementation, and bioluminescent and fluorescent-based assays.
Z378178 Handbook of Capillary Electrophoresis, 2nd ed. This fully updated edition pulls together diverse areas and applications of CE; the result is a volume that serves scientists in biotechnology, clinical, and pharmaceutical chemistry. The clear organization distinguishes general information − theory, modes of CE, detection, and data analysis − from specifics − with chapters on small molecules, peptides, proteins, carbohydrates, and nucleic acids as analytes. Some of the most useful chapters explore highly specialized applications of CE to large-scale DNA sequencing, drug analysis in biological fluids, binding studies, and enzyme assays.
Z540838 Handbook of Chemical Health and Safety This handbook provides the tools necessary to create and maintain a safe work environment. It provides a starting point for any chemical safety study, from providing information on proper chemical and equipment handling to guidance on purchasing, storage, use and disposal. This is the single most useful how-to guide available on issues related to chemical safety and will be an absolute necessity in any lab, chemist, chemical hygiene officer, or safety specialist’s workplace.
Z705284 Handbook of Chiral Chemicals, 2nd ed. This edition highlights the problems associated with the production of chiral compounds on a commercial scale. The handbook first elaborates upon starting materials obtained from a "chiral pool," which can be derived from natural products. Then it explains methods and reactions that can introduce or influence stereogenic centers, particularly asymmetric hydrogenations, oxidations, pericyclic reactions, and enzymatic methods. The search for the ideal catalyst has consistently led researchers to enzymes present in biological systems. Several chapters concentrate on understanding how to manipulate enzymes for catalyzing new reactions for taking new substrates. Other topics include chiral auxiliaries, chromatographic techniques, enantiomers-specific reactions, and resolution.
Z424234 Handbook of Derivatization Reactions for HPLC, Book and CD-ROM This print and electronic database represents a tremendous time-saver for analytical chemists working in the popular area of HPLC. By abstracting and evaluating the most important derivatization procedures, chemists can avoid lengthy information searches to consult the primary literature. Both the print and fully-searchable CD-ROM databases offer everything an analytical chemist needs to conduct derivatizations.
Z702277 Handbook of Detection of Enzymes on Electrophoretic Gels, 2nd ed. This edition makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a tool proven successful in solving a variety of problems. New is an overview of applications of enzyme electrophoresis and zymogram techniques; zymogram techniques for approximately 100 enzymes not in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes; information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms; an appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels.
H1037 Handbook of Drug Screening This reference provides coverage of current screening techniques, modern technologies, and high-capacity instrumentation for development and discovery of new drugs, chemical compounds, and targeted delivery of pharmaceuticals. It describes the construction and compilation of chemical compound databases and clinical trials. It contains applications and examples of strategies in cell-based and cell-free screens, as well as homogeneous, fluorescence, chemiluminescence, and radioactive-based technologies It covers identification of transcriptional factors for drug targeting; affinity-based approaches to screening; the role of proteomics and bioinformatics in target identification; laboratory automation and assay miniaturization in ultrahigh-throughput screening.
Z704164 Handbook of ELISPOT: Methods and Protocols In this book on ELISPOT, a critical enzyme-linked immunospot assay, experts detail how to design, perform, and analyze these assays. The techniques cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
Z525375 Handbook of Environmental Data on Organic Chemicals, 2 vol. set, 4th ed. For over two decades, the Handbook of Environmental Data on Organic Chemicals has provided comprehensive, in-depth coverage of
dangerous organic compounds along with the control measures designed to reduce their destructive impact on the ecosystem. This
new edition is fully updated to reflect the latest research in the field, including new organic chemicals, mixtures, and preparations.
Z704822 Handbook of Flavors and Fragrances This reference contains detailed information on more than 4000 natural and synthetic trade name and generic chemicals that are used in the flavor and fragrance industry. Flavoring agents are used in the formulation of food, pharmaceuticals, and cosmetics. Fragrances, aroma chemicals, and perfumes are used in these industries as well as other consumer and industrial products in order to mask unpleasant odors. Entries in this reference for both trade names and generic chemicals contain extensive information on identification, synonyms, odor and/or flavor descriptions, molecular formula, chemical properties, function and application, toxicology, precautions, usage level, and regulatory data. Detailed contact information on suppliers of these chemicals/materials is also included. This invaluable research tool will provide easy access to information for all chemical professionals involved in the use of flavors and fragrances in compounding and formulation.
Z704520 Handbook of Fluorous Chemistry This handbook is the first to summarize all the essential aspects of this emerging field of chemistry. Whether the reader is seeking an introduction to the concept of fluorous biphase catalysis, summaries of partition coefficients involving fluorous and organic solvents, or information on the latest fluorous mixture separation techniques, this authoritative compilation provides key information needed for successfully working with the diverse and fascinating families of fluorous molecules. The large number of reliable experimental procedures in particular makes this the ideal guide for newcomers wanting to use this elegant method in the laboratory.
Z553824 Handbook of Free Radical Initiators This book presents the physicochemical data on radical initiators and reactions of radical generation. Free radical initiators serve as reactive intermediates in synthetic methodologies such as organic and polymer synthesis as well as in technological processes, oligomerization, network formation, and kinetic research
Z278998 Handbook of Grignard Reagents This useful handbook provides the theoretical and practical information necessary to explore new applications for Grignard
reagents on a day-to-day basis. Presents a comprehensive overview of current research activities in Grignard chemistry.
Surveys specific reactions and applications of Grignard reagents, organized by type of substrate and the general category of reaction.
Z375632 Handbook of Human Immunology This book provides a complete guide to molecular immunology. The basic principles underlying assays of the immune system are discussed as are the application and interpretation of immune tests. It emphasizes the molecular basis for induction and regulation of immune responses; discusses cell receptors and accessory molecules, cytokines, growth factors and interleukins and their application in the diagnosis of various diseases; reviews serological testing for infectious diseases and the application of molecular biological techniques; and 115 tables summarize textual information.
Z700509 Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells The authors describe the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.
Z513695 Handbook of Industrial Crystallization, 2nd ed. The authors provide an introduction to the field of newcomers and a reference to those involved in the various aspects of industrial crystallization. It is a complete volume covering all aspects of industrial crystallization, including material related to both fundamentals and applications. This new edition presents detailed material on crystallization of biomolecules, precipitation, impurity-crystal interactions, solubility, and design.
Z730599 Handbook of Industrial Surfactants 4th ed, CD-ROM • More than 28,000 trade name products
• More than 5,000 generic products with fully cross-referenced synonyms (hyperlinked in software)
• HLB Classification Index
• Ionic Classification Index
• More than 19,000 Functional/Application Categories in a detailed index format
• CAS, EINECS/ELINCS number and Chemical Formula index
• More than 4,000 International Manufacturers
Z730068 Handbook of Industrial Surfactants, 4th ed. 2 volume set • More than 28,000 trade name products
• More than 5,000 generic products with fully cross-referenced synonyms (hyperlinked in software)
• HLB Classification Index
• Ionic Classification Index
• More than 19,000 Functional/Application Categories in a detailed index format
• CAS, EINECS/ELINCS number and Chemical Formula index
• More than 4,000 International Manufacturers
Z552240 Handbook of Infrared Spectroscopy of Ultrathin Films Because of the rapid increase in commercially available Fourier transform infrared spectrometers and computers over the past ten years, it has now become feasible to use IR spectrometry to characterize very thin films at extended interfaces. At the same time, interest in thin films has grown tremendously because of applications in microelectronics, sensors, catalysis, and nanotechnology. This book provides a practical guide to experimental methods, up-to-date theory, and considerable reference data, critical for scientists who want to measure and interpret IR spectra of ultrathin films.
Z703443 Handbook of Ion Chromatography, 3rd Completely revised edition The third edition of this highly successful handbook has been completely revised and considerably extended, making it unrivaled in the timeliness and comprehensiveness of the information presented. This new edition runs to two volumes, with added chapters or sections that cover new and important applications of ion chromatography in the life sciences, such as the analysis of proteins, nucleic acids, amino acids or carbohydrates. Also, new instrumentation that meets the demand for miniaturization and reduced analysis times; Coupling of ion chromatography to mass-spectrometric or inductively coupled plasma detectors; and Validation of ion-chromatographic methods, which is important for quality assurance.
Z547689 Handbook of Luminescence, Display Materials, and Devices Provides coverage on organic light emitting diodes (OLEDs) and inorganic display devices including materials synthetic strategies, processing and fabrication methods, screening methods, spectroscopic characterization, energy transfer processes, luminescence in conjugated oligomers, polymers, nanostructured materials, carbon nanotubes, flexible display technologies, up-conversion phosphor materials and aging process, emissive displays, display device reliability, electrode material degradation, packaging, surface properties, etc. The three volumes include: Organic Light-Emitting Diodes; Inorganic Display Materials; Display Devices
Z551570 Handbook of Metathesis, 3 volume set There is probably no name more closely linked to metathesis than that of Robert H. Grubbs of the California Institute of Technology. His pioneering work has led to the success of this important and fascinating reaction and in this comprehensive three-volume work he presents all its important aspects. The standard work on the topic. The team of authors reads like a "Who's who" of metathesis. Clearly divided into catalyst developments, organic synthesis applications and polymer synthesis
Z702714 Handbook of Microbiological Media, 3rd ed. The third edition lists the formulations, methods of preparation, and uses for more than 4,000 microbiological media. With almost 30 percent more entries than the previous edition, the book includes new information on international collections of microorganisms. It also contains formulations and uses of media for isolation, culture, identification, and maintenance of microorganisms. The entries are arranged alphabetically by medium name and include synonyms, sources, and more. It makes finding media for culturing diverse microorganisms quick and simple, and presents easy-to-follow directions and "cookbook recipes" for preparing media.
H4161 Handbook of Modern Pharmaceutical Analysis This book describes the role modern pharmaceutical analysis plays in the development of new drugs. Detailed information is provided as to how the quality of drug products is assured from the point of discovery until the patient uses the drug. Coverage includes state-of-the-art topics such as analytics for combinatorial chemistry and high-throughput screening, formulation development, stability studies, international regulatory aspects and documentation, and future technologies that are likely to impact the field. Emphasis is placed on current, easy-to-follow methods that readers can apply in their laboratories.
Z702366 Handbook of Molecular and Cellular Methods in Biology and Medicine, 2nd ed. With step-by-step protocols, this edition presents up-to-date molecular and cellular methods and techniques, including additional methods for analysis of DNA-protein interactions by DNA footprinting and gel retardation assay, mRNA differential display, and microarray technology; Explains the principles behind each method; Includes helpful trouble-shooting guides. This edition includes: new protocols in all chapters, including alternative protocols; In vitro transcription methods; analysis of DNA sequences; new bioseparation techniques. New chapters cover: mRNA differential display; Inhibition of gene expression; In situ hybridization; Combinatorial techniques; Computational data mining methods applied to combinatorial chemistry libraries.
Z550132 Handbook of Organic-Inorganic Hybrid Materials and Nanocomposites Covers molecular building blocks for preparing hybrid materials and nanocomposites, different synthetic routes allowing multifunctionality with a wide range of composition, sol