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Z730521
What’s Wrong With My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd ed.
Turn to
What’s Wrong with My Mouse?
to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, it includes two new chapters: "Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis. "Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
W2390
What’s Wrong with My Mouse Behavioral Phenotyping of Transgenic and Knockout Mice
This concise review explains the differences between transgenic and knockout mice and their wild-type controls, while providing critical information about gene function and expression. Newly identified genes can provide insights into brain malfunctioning in disease states. It discusses how to generate a transgenic or knockout mouse, functions (open field, holeboard, rotarod, balance, grip, circadian activity, etc.), sensory abilities (olfaction, vision, hearing, taste, touch, nociception), reproductive behavior, social behavior, and emotional behavior.
Z700029
Wheater’s Basic Histopathology: A Color Atlas and Text, 4th ed.
This atlas and text of histopathology provides a practical collection of color photomicrographs of diseased human tissue to supplement standard pathology texts. An ideal companion to "Wheater's Functional Histology," this new edition gives more emphasis to the scientific basis of disease and clinicopathological correlations.
Z701742
When Cells Die II: A Comprehensive Evaluation of Apoptosis and Programmed Cell Death
This edition offers the most thorough coverage of this field. Leading researchers present an up-to-date survey ranging from the history of cell death science to its modern methodology. Revised to include major advances in research, this edition features relevant discussion of: The impact of genomics and proteomics; Gene therapy and pharmacogenetics; Role of mitochondria; Caspase-independent and non-apoptotic cell death; Evolution of mechanism. With the manipulation of programmed cell death in clinical situations, this book also addresses the role of apoptosis in specific organ systems; the immune system, nervous system, and gastrointestinal tract; as well as different disease states, including viral infection, cancer, and myocardial infarct.
Z719218
Whole Genome Amplification: Methods Express Series Softcover
Using Whole Genome Amplification (WGA) methods, it is possible to create microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This title provides a comprehensive overview of the field and will be welcomed by all researchers looking to take advantage of the latest developments.
Z553484
Wiley Guide to Chemical Incompatibilities
Compiles hard-to-find data on over 11,000 chemical compounds, describing a wide range of chemical reactions that produce undesirable results in non-controlled situations.
Z705128
Xenopus Protocols: Cell Biology and Signal Transduction
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.
Z704911
Xpression Primer
A revolutionary tagged primer design tool for expression cloning.
•
High throughput primer design-
Use Xpression Primer's sophisticated algorithm to design thousands of primers in a single search run. The program uses statistical optimization techniques to select the best primer pair for each sequence based on your selection criteria.
•
Extensive Assay Support-
Xpression Primer provides comprehensive support to high throughput cloning systems such as Gateway™, BD In-Fusion, epitope and TOPO™ tools. To work with other expression systems, add functional tags of your choice and design tagged primers.
•
Sophisticated Sequencing Primer design-
Xpression Primer designs optimal primers for multiples sequences in a single search run.
•
Generate Precise transcripts-
Design primers to generate sense or antisense transcripts for in vitro expression studies.
•
Web Savvy-
Xpression Primer searches Entrez and downloads batches of ORF sequences directly into the program.
•
Lightning fast-
Processes three hundred 10kb sequences in approximately 2 minutes.
Z368695
YAC Protocols
This book presents step-by-step protocols covering all aspects of yeast artificial chromosomes, ranging from the construction of YAC libraries to their storage, screening and database management, and to their use in such specialist applications as the transfer of YACs to mammalian cells and the isolation of coding sequences from YACs.
Y4002
Yeast Hybrid Technologies
Two-hybrid system provides a novel way of detecting protein-protein interactions through a powerful genetic approach. This approach has been well adapted to various other purposes, such as detecting protein-DNA interactions (one hybrid) and RNA-protein interactions or multiple protein interactions (three-hybrid). It provides detailed laboratory protocols, basic technology, variations, special applications, and troubleshooting suggestions. There is also useful information on in vitro protein interaction detection methods, and both bacterial and mammalian two-hybrid systems.
Z704776
Yeast Protocols, 2nd ed.
This edition is a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components,including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Z378062
Yeast Two-Hybrid System
This volume, part of the Advances in Molecular Biology series, presents detailed protocols and trouble-shooting advice on the yeast two-hybrid system, one of the most powerful and versatile methods for characterizing a protein's functions. Topics include: characterizing hormone/receptor complexes, identifying peptide ligands, how to identify mutations that disrupt an interaction, how to construct an activation domain hybrid library, and how to dissect the cell cycle and other complex genetic networks.
Z700398
Zebrafish: A Practical Approach
The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory, and also gives a broad overview of commonly used methods and a collection of protocols describing the most powerful techniques.
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