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Z703567
Understanding Mass Spectra: A Basic Approach
This edition combines coverage of the principles underlying mass spectral analysis with clear guidelines on how to apply them in a laboratory setting. Completely revised, it provides an updated and unified approach to mass spectral interpretation that emphasizes the application of basic principles. A detailed overview of theory and instrumentation, this useful guide contains step-by-step descriptions of interpretative strategies and convenient lists and tables detailing the information needed to solve unknowns. Other features include real-world case studies and examples, skill-building problems with clearly explained answers, and easy-to-follow explanations of the important mathematical derivations.
A8968
Using Antibodies: A Lab Manual
Harlow and Lane have revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been rewritten, reorganized, and updated to provide background, context and step-by-step instructions. Techniques range from choosing the right antibody and handling it correctly to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, this title instead identifies in each case the best approach to specific problems.
Z703699
Using Mass Spectrometry for Drug Metabolism Studies
Mass spectrometry is fast becoming the premier tool for analyzing various drug metabolism samples in the early phases of drug discovery and research. Introducing the newer, more powerful MS equipment and exploring new applications for using them, this book provides a state-of-the-art look at this field. It offers current knowledge in stand-alone chapters that address specific topics with notes and references to other chapters for further reading. The first eight chapters discuss current topics regarding the use of MS for analyzing various types of in vitro and in vivo drug metabolism samples and the final four chapters describe the latest MS technology and its uses. In each chapter, the authors demonstrate how to apply MS to determine drug metabolism parameters, and also explain the different drug metabolism concepts and their importance.
Z705896
Vaccine Adjuvants: Immunological and Clinical Principles
A cutting-edge review of the major research areas of adjuvant discovery, design, development, and use. The authors lay down a rational basis for vaccine adjuvant function and analyze a number of significantly distinct adjuvant-active molecules to illuminate the principles of their function and use. The focus is on specific receptor-ligand interactions, including the molecular features needed for a compound to possess adjuvant activity. The critical interface zone between the innate and adaptive immune systems is also analyzed to show how adjuvants exert their effects on T- and B-cell activation. Additional chapters address the possibility of tailoring adjuvants to yield optimally safe and effective responses.
Z730157
Validating Chromatographic Methods: A Practical Guide
This guide focuses on high-performance liquid chromatographic methods validation; however, the concepts are generally applicable to the validation of other analytical techniques as well. Following an overview of analytical method validation and a discussion of its various components, the author dedicates a complete chapter to each step of validation: Method evaluation and further method development; Final method development and trial method validation; Formal method validation and report generation; Formal data review and report issuance. Templates and examples for Methods Validation Standard Operating Procedures, Standard Test Methods, Methods Validation Protocols, and Methods Validation Reports are all provided. Moreover, the guide features detailed flowcharts and checklists that lead readers through every stage of method validation to ensure success. All of the templates are also included on a CD-ROM, enabling readers to easily work with and customize them.
Z379891
Vectors, Cloning Applications: Essential Techniques Series
A quick reference to the basic cloning procedures that must be mastered before DNA characterization or manipulation can be attempted. Includes in vitro transcription, cloning of very large DNA fragments for library construction, cloning of PCR products, and selected downstream manipulation techniques.
V1763
Virtual Screening for Bioactive Molecules
Screening techniques are very common in biochemistry and biology to find out if new molecules are potential drugs. The huge amounts of new potential drugs are tested in most of the cases in a screening in view of only one special disease or one special function. There is not enough human power or financial resources to test these substances with common screening methods in order to know if they are active or helpful. Virtual screening can help industry to increase their efficiency drastically by estimating the activity of compounds not in an experiment, but with the computer.
Z340898
Virus Culture: A Practical Approach
This book provides a broad treatment of the principles and practice of virus culture. The first section is an overview of cell culture techniques essential for virologists. Other techniques covered are isolating, identifying, concentrating, and purifying viruses. Electron microscopy as applied to virology is explained. Topics include creating virus vaccines, antiserum production, monoclonal antibodies, antiviral drug testing and methods to study plant viruses.
V1888
Visualization of Receptors in situ: Applications of Radioligand Binding
Radioligand binding in situ is the most sensitive method for detecting available receptors. But the success and reliability of this approach are determined by a number of technical requirements and constraints. This book combines theory and practice. It covers procedures such as radioligand and tissue preparations, in vitro assays, autoradiographic emulsion handling and image analysis. It also provides detailed protocols and uses parallel columns to convey practical comments and advise, often not encountered in original research papers.
Z274054
Vogel’s Qualitative Inorganic Analysis, 7th ed.
Discusses experimental techniques, reactions of ions, and selected tests and separations.
Z506931
Vogel’s Textbook Of Quantitative Chemical Analysis, 6th ed.
Includes many organic applications and experiments. Topics covered include: fundamentals; errors, statistics, and sampling; separative techniques, titrimetry and gravimetry, and electroanalytical and spectroanalytical methods.
Z203947
Vogel’s Textbook of Practical Organic Chemistry, 5th Ed.
This edition retains the comprehensive, reference-text character, updated, with the introduction of new reagents and techniques as well as the development of a philosophy of organic synthesis, integrating mechanistic theory with the strategy and methodology of synthesis. Chapters deal with experimental techniques, and spectroscopic methods, as well as specific aliphatic, aromatic, alicyclic, heterocyclic, and organic compounds.
Z705551
Voltage Gated Ion Channels as Drug Targets
Edited by the most prominent person in the field and top researchers at US pharmaceutical companies, this is a unique resource for drug developers and physiologists seeking a molecular-level understanding of ion channel pharmacology. After an introduction to the topic, the authors evaluate the structure and function of ion channels, as well as related drug interaction. A section on assay technologies is followed by a section each on calcium, sodium and potassium channels. Further chapters cover genetic and acquired channelopathies, before the book closes with a look at safety issues in ion channel drug development.
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