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Z700622 E. coli Gene Expression Protocols This book is a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The author's focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.
E2027 ELISA Guidebook This is today's premier practical guide to the understanding and application of ELISA. Updating Crowther's publication, ELISA Theory and Practice (1995), this book introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. It contains numerous worked examples, detailed instructions, and extensive illustrations.
Z511854 Electrochemical Methods: Fundamentals and Applications, 2nd Edition This edition is fully revised to reflect the current state off the field. Significant additions include ultramicroelectrodes, modified electrodes, and scanning probe methods. Many chapters have been modified and improved, including electrode kinetics, voltammetric methods, and mechanisms of coupled chemical reactions. Takes the reader from the most basic chemical and physical principles through fundamentals of thermodynamics, kinetics, and mass transfer, to a thorough treatment of all important experimental methods. Treats application of electrochemical methods to elucidation of reaction mechanisms; double layer structure and surface processes, and their effects on electrode processes are developed from first principles; other key features include a chapter on operational amplifier circuits and electrochemical instrumentation, unique coverage of spectrometric and photochemical experiments, and Laplace transform and digital simulation techniques.
Z235776 Electron Flow in Organic Chemistry With the goal of helping students develop a good intuition for organic chemistry, it approaches the material from a mechanistic viewpoint. Presents twenty electron flow pathways as the building blocks of all the common mechanistic processes. Thus, students deal with a smaller number of reactant classes instead of studying each reaction as a separate case. Uses physical models such as energy surfaces to aid the decision-making process; includes a unique chapter that teaches students how to make a multivariable decision; and contains advanced explanations using interaction diagrams and molecular orbital theory.
E8025 Electron Microscopy Methods and Protocols Experts describe key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological x-ray microanalysis, this book includes methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Immunogold labeling techniques for ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in detail to provide insight into their advantages and limitations.
E8890 Electron Microscopy: Principles and Techniques for Biologists. 2nd ed. A direct, uncomplicated introduction to electron microscopy for biologists. This second edition has enhanced layout, illustrations, and text so that it is more accessible to the reader. Over 200 new line drawings and micrographs have been added.
Z422940 Electronic Processes in Organic Crystals and Polymers, 2nd ed. This edition contains the complete text of the first edition, plus an extensive new section, comprising nearly half of the book, which covers recent developments and applications with polymers. The book provides a unified description of what is known in almost every aspect of the field, from basic phenomena to the practical applications, which include LED's, photocopiers, photoconductors, batteries, transistors, liquid crystals, photorefractive devices, and sensors
E1404 Embryonic Stem Cells: Methods and Protocols This book describes productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. These detailed protocols range from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, to the generation of antibodies against very rare antigens, and to the identification of and characterization of protein and protein interactions. This will prove an invaluable resource for those actively using, or planning to use ES cells, to study fate choices and specific lineages.
E3778 Emery’s Elements of Medical Genetics, 11th ed. This book has been the world's most trusted genetics text for more than 30 years. With balanced, integrated reporting on both the basic science and clinical implications of genetics, the content has been thoroughly updated for this edition to keep abreast of this fast-changing subject. There is new coverage of hot topics such as gene therapy, the human genome project and ethical issues in medical genetics, while a brand new illustration program has been specially designed to further help students to understand concepts and retain information.
Z289418 Emulsion Polymerization and Emulsion Polymers Emulsion polymerization is used commercially to produce synthetic alternatives to natural latex rubber. This book provides an up-to-date treatment of all aspects of the field--including basic theory, practice, major industrial uses and related technologies.
Z422436 Enantiocontrolled Synthesis of Fluoro-Organic Compounds: Stereochemical Challenges and Biomedical Targets Selective insertion of fluorine substituents in strategic positions of bioactive molecules is a powerful and versatile tool for the design of new drugs, diagnostic agents, agrochemicals and biomedical probes. This is the first book to give a comprehensive overview of the preparative methods for enantiocontrolled synthesis of fluorine-containing compounds of biomedical importance.
Z704652 Enantioselective Synthesis of Beta Amino Acids, 2nd ed. Beta amino acids are studied in research areas such as combinatorial chemistry, medicinal chemistry, molecular design, proteomics, and others. This second edition updates reviews, covers new developments, and presents detailed discussions of the most important methods for the synthesis. In addition, this book features introductory overviews on the structural types of relevant beta-amino acid targets and salient beta ­amino acids present in natural products; Dedicates several chapters to advances in the synthesis of oligomers from beta-amino acids; Discusses the most important methods that have been recently developed for the asymmetric synthesis of cyclic and open-chain beta-amino acids; and Includes a report on the preparation of libraries of enantiopure beta-amino acids using combinatorial approaches.
Z271780 Encyclopedia of Common Natural Ingredients Used in Food, Drugs, and Cosmetics, 2nd ed. The second edition stands as the most authoritative reference for natural ingredients in commercial use. It encompasses approximately 500 of the most commonly used ingredients, and covers the identification, processing, preparation, and manner of use for each. This volume emphasizes the drugs and herbal formulations created for those purposes. It updates all the original entries of the first edition, and incorporates many new entries, classifications, references, and a new category, Health Foods/Herbal Teas. The features include In-depth coverage of each ingredient, including general description, chemical composition, uses and commercial preparations, and synonyms, as well as regulatory status and references; An easily accessible alphabetical presentation of the entries according to common names, and cross-referenced to scientific names in the index; Glossary of the most commonly encountered terms used in the botanical industry.
Z248053 Encyclopedia of Reagents for Organic Synthesis, 8-volume set This essential reference contains an authoritative and systematic description of the use of all reagents in organic chemistry. It includes approximately 3500 alphabetically arranged articles, a comparison of reagents with others capable of similar chemistry, a pro and con assessment for each reagent, extensive cross-referencing and substantial subject and molecular formula indexes.
Z286788 Encyclopedia of Separation Technology Separations technologies on an industrial scale comprise over 50 percent of the capital and operating costs in industry. This two-volume encyclopedia, based on the authoritative Kirk-Othmer Encyclopedia of Chemical Technology, provides A to Z coverage of the various techniques that can be used to effect separations of gases, liquids, and solids for the purification of new materials, feedstocks, process streams, or finished products. Written by experts in the field, entries provide valuable information on both the technology and the underlying science of separations. This book brings together all of the authoritative articles on separation technology from the main Kirk-Othmer Encyclopedia into a thoroughly updated, easily accessible two-volume set; Contains over 30 percent new and updated material; Includes five new specially commissioned articles on key topics - such as bioseparations, contactors for separations processes, and high purity gases
Z379689 Endothelin: Molecular Biology, Physiology, and Pathology Academic, clinical, and pharmaceutical researchers summarize the dramatic recent progress in endothelin research and speculate on future studies. The respected contributors critically evaluate and synthesize our knowledge of endothelin chemistry, its mechanism of action, and its physiological function. Biochemists studying the role of endothelin in cardiovascular physiology and medicinal chemists developing endothelin-centric drugs will find the book rich in insights.
Z378151 Endothelins in Biology and Medicine The latest findings on function, effects and characteristics of endothelins (ETs), meticulously researched and thoroughly reviewed. Five sections cover ET receptors, synthesis and degradation, pathophysiology, structure-activity relationships, and clinical studies. Almost 2,000 citations to current literature put further resources at your fintertips.
E5027 Endotoxins: Pyrogens, LAL Testing, and Depyrogenation, 2nd ed. This revised edition focuses on environmental prevalence, detection, properties, and biological structure and function of endotoxins from a parenteral manufacturing perspective, including in-depth discussion of state-of-the-art technologies involving Limulus amebocyte lysate (LAL), such as assay development, automation, and depyrogenation. It surveys shift in emphasis away from pyrogens to endotoxins and development and proliferation of semiautomated kinetic LAL assays. With regulatory references and analytical protocols, this book emphasizes application of contamination control in pharmaceutical industry and details instances of endotoxin parenteral contamination.
E6514 Enzfitter brochure
E0401 Enzfitter Windows version EnzFitter is a generic curve-fitting package which has custom features designed to make it especially suitable for analysis of enzyme kinetics experiments. For example, initial rate and parameter values can be obtained with their confidence limits for single and twin substrate rate data. Built-in models include Michaelis-Menten with or without substrate inhibition, competitive, uncompetitive and mixed inhibition, ternary complex or ordered bi-bi systems and ping-pong with and without inhibition by substrates. You can easily add other models in conventional algebraic syntax
EnzFitter can be set up to run in batch mode (performing several analyses automatically, without user intervention) and it also supports OLE Automation. For more general assay use, you can calculate unknowns from fitted standard curves. You can create text Notes that are attached to any file, dataset or curve fit. There is also an automatic Logbook which records all changes made to a given file. The 32-bit version supports Regional settings for things like decimal points. There is comprehensive Help and a manual
E8778 Enzyme Assays: A Practical Approach, 2nd ed. This edition combines revised chapters with new contributions in describing the design and execution of enzyme assays. Topics include protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. Theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high-throughout screening; statistical analysis of enzyme kinetic data; and determination of active site concentration
Z704962 Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting Nowadays, the characterization of enzymes using simple assays is a key technology for finding the optimum enzymes for, among others, catalysis in enzymology and drug discovery. Thus, enzymes have become an everyday tool not only for biologists, but also for chemists, and the number of applications is now overwhelming. This book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.
Z411256 Enzyme Chemistry: Impact and Applications, 3rd ed. The previous editions of this book established it firmly as an invaluable starting point and concise reference source for chemists, biochemists, microbiologists and others seeking an overview of enzyme chemistry and a gateway to the surrounding literature. This new edition covers these advances in understanding while retaining the basic structure which has proved so effective in earlier years. The aim remains the same: to provide those graduates with some knowledge of chemistry but a limited knowledge of enzymes, with a one-stop access point to this strategically critical field.
Z370258 Enzyme Immunoassays: From Concept to Product Development A vertical exposition of enzyme immunoassays; the intended audience is product designers and R&Dgroups who aim to commercialize EIA technology. From concepts of antibody and antigen through laboratory development, all the way to manufacturing, marketing, and regulatory compliance, the book is a practical resource for the diagnostic field.
Z700355 Enzyme Kinetics and Mechanism, Part F: Detection and Characterization of Enzyme Reaction, Methods in Enzymology Vol 354 In this volume of Methods in Enzymology, every chapter is devoted to the task of detecting and characterizing enzyme reaction intermediates. This volume contains detailed descriptions of a broad range of experimental approaches that are applied to a diversified group of enzymes and reaction types. Key features of this volume include Spectroscopic Detection of Reaction Intermediates; Isotopic and Kinetic Detection of Reaction Intermediates; Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates.
Z700363 Enzyme Kinetics: A Modern Approach This book provides a guide to evaluate enzyme kinetics, using common software packages to perform enzymatic analyses. The treatment of enzyme kinetics here is radically different. Marangoni stresses an understanding of how researchers arrive at models, what the models' limitations are, and how they can be used in ways to analyze enzyme kinetic data. Computers make linear transformations of models unnecessary, and this book does away with them, advancing the use of nonlinear regression techniques. Marangoni develops new ways to carry out analyses of enzyme kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes.
E2529 Enzyme Kinetics: Principles and Methods This is a user-friendly and comprehensive treatise on enzyme kinetics, indispensable for biochemists, biologists, medical scientists, and chemists working with enzymes. Theory and practice are well balanced, the relation to the biological system is always emphasized. Theoretical aspects are presented in a way, which is also comprehensible for the beginner. An extensive methodological part provides the expert with valuable support in planning and performing laboratory experiments. With each copy is a CD-ROM with EKI-3, the elaborate and easy-to-use version of the enzyme kinetics practical course.
E3903 Enzyme Technologies for Pharmaceutical and Biotechnological Applications This review of enzyme function in human and animal health covers basic principles and applications in antibiotic biosynthesis, biocatalysis, screening and assay optimization, and new technologies. It offers examples of laboratory, pharmaceutical, and manufacturing processes. Emphasizing modern methods of metabolic engineering and drug discovery processes, it details enzyme targets related to human disease, development of effective enzymatic assays, methodologies in high-throughput screening, discovery of novel compounds for lead generation, improvements in antibiotic yield, and generation of contemporary protein products.
Z704172 Enzymes and Their Inhibition: Drug Development Once an enzyme target is selected or discovered for a particular disease or infection, detailed information must be compiled for the rational design of an inhibitor. This book examines enzyme inhibition in detail, and describes the design and development of such inhibitors. It provides an overview of the chemistry of major types of enzymes and their inhibitors. The opening chapters introduce readers to the structure, substrates, kinetics, and mechanisms of enzymes. Subsequent chapters discuss various types of enzyme inhibitors and the principles involved in developing them into effective drugs. This book describes the problems that can be encountered in translating results from the bench to the market place, and suggests approaches that circumvent these difficulties
E9017 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis, 2nd ed This book explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. New features include a new chapter on protein-ligand binding equilibria, expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity, updated discussions of enzyme inhibitors and multiple substrate reactions, and coverage of current practical applications to the study of enzymology.
Z705101 Epidermal Growth Factor: Methods and Protocols A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols format offers step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls
Z730041 Epigenetics The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals..
E2029 Epithelial Cell Culture Protocols A team of experimenters and researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria.
Z369950 Epithelial Cell Culture: A Practical Approach Cells in culture can sometimes organize into tissue-like structures. In vitro epithelial ¿tissue¿ serves as a useful model system for studying pharmacology, toxicology, and cell biology. Written by an international panel of researchers, this book details the methods for establishing such a culture and describes some interesting research applications.
Z373990 Epitope Mapping Protocols: Methods in Molecular Biology, Vol. 66 This manual offers more than thirty protocols for antibody production and mapping. Methods range from those that use (or can use) whole, native antigens to those that require antigen expressed from recombinant DNA. Methods for T-cell epitopes and techniques for producing panels of antibodies for epitope mapping are also included.
E4527 Epitope Mapping: A Practical Approach This book covers major methods for identification and definition of epitopes. Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides. Site directed mutagenesis is a powerful tool and can be used to evaluate the role of single amino acids in immune complex formation. Protein footprinting relies on the fact that the epitope is protected from cleavage when bound as an antibody-antigen complex. Random phage display libraries can be screened with antibody and resulting DNA sequenced to confirm the amino acid sequence of a specific epitope.
Z700703 Erythropoietin: Molecular Biology and Clinical Use Erythropoietin has been the fountainhead in research on molecular mechanisms of oxygen-sensing and hypoxia-induced gene expression. The availability of recombinant human EPO for prevention and therapy of chronic anemias was a turning point in clinical medicine. This book intends to provide a fairly complete overview and bibliography of the progress made in important areas of basic and clinical EPO research. It is divided into sections on EPO structure and control of production; EPO mechanisms of action; EPO physiology and pathophysiology; EPO pharmacology and therapy; and recombinant human EPO therapy.
Z702889 Essential Cell Biology 2nd ed. The second edition contains basic, core knowledge about how cells work. The text and figures have been prepared to be easy-to-follow, accurate, clear and engaging for the introductory student. Each section follows logically from the previous one, telling a story, rather than being a collection of facts. Questions integrated throughout each chapter encourage the reader to pause, think about what they have read, and attempt to apply the new knowledge in ways that test their understanding. Based on user feedback, this edition now offers increased coverage of genetics and more experimental background.
Z701092 Essential Cell Biology: A Practical Approach 2 Volume set The challenge was not to compile a collection of all the techniques available in cell biology, but to identify those methods that are key to research progress and most relevant to researchers new to the topic.