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Atto Labels

Atto labels are designed for high sensitivity applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding assays, or enzyme assays. Secondary antibody, streptavidin, and biotin conjugates are available for most of the Atto labels.
  • Spectral data: Emission maxima cover a broad range from 500 to 700 nm
  • Stoke's shift: Shift between absorption maxima and emission maximum is sufficient to discriminate emission from excitation
  • Atto labels exhibit bright fluorescence, extraordinary photostability and good hydrophilicity

Atto Label Technical Details

Product #
Description
Assay
Fluorescence
89313 Atto 390 ≥98.0% (HPCE)
 λex 390 nm; λem 475 nm in 0.1 M phosphate pH 7.0
89204 Atto 390 NHS ester ≥80% (coupling to amines)
 λex 389 nm; λem 471 nm in 0.1 M phosphate pH 7.0
89740 Atto 390 maleimide ≥80% (coupling rate)
 λex 390 nm; λem 472 nm in 0.1 M phosphate pH 7.0
16805 Atto 425-NHS ester λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
49349 Atto 425-maleimide ≥70% (coupling to thiols)
 λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0
50712 Atto 465
55607 Atto 465 maleimide ≥98.0% (HPCE)
 λex 452 nm; λem 502 nm in 0.1 M phosphate pH 7.0
53404 Atto 465-NHS ester ≥80% (HPCE)
 λex 449 nm; λem 503 nm in 0.1 M phosphate pH 7.0
41051 Atto 488 ≥90% (HPCE)
 λex 504 nm; λem 521 nm in 0.1 M phosphate pH 7.0
28562 Atto 488 maleimide ≥90% (HPCE)
 λex 501 nm; λem 523 nm in 0.1 M phosphate pH 7.0
41698 Atto 488-NHS ester ≥80% (coupling to amines)
 λex 498 nm; λem 520 nm in 0.1 M phosphate pH 7.0
16951 Atto 495 ≥98.0% (HPCE)
 λex 499 nm; λem 535 nm in 0.1 M phosphate pH 7.0
41022 Atto 495 maleimide ≥98.0% (HPCE)
 λex 500 nm; λem 524 nm in 0.1 M phosphate pH 7.0
00379 Atto 495-NHS ester ≥90% (coupling to amines)
 λex 499 nm; λem 524 nm in 0.1 M phosphate pH 7.0
70706 Atto 520 ≥90.0% (HPCE/HPLC)
 λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
77810 Atto 520 NHS ester ≥80.0% (coupling to amines)
 λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
16590 Atto 520-maleimide ≥90% (HPCE)
06699 Atto 532 ≥90% (HPCE)
 λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
88793 Atto 532 NHS ester ≥80% (coupling to amines)
 λex 530 nm; λem 551 nm in 0.1 M phosphate pH 7.0
68499 Atto 532 maleinimide ≥90% (HPCE)
 λex 532 nm; λem 553 nm in 0.1 M phosphate pH 7.0
40592 Atto 540 Q ≥98.0% (HPCE)
61683 Atto 540 Q NHS ester ≥90% (coupling to amines)
62453 Atto 540 Q maleimide ≥98.0% (HPCE)
42424 Atto 550 ≥90% (HPCE)
 λex 554 nm; λem 574 nm in 0.1 M phosphate pH 7.0
92835 Atto 550 NHS ester ≥80% (coupling to amines)
 λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0
30730 Atto 550 maleimide ≥90% (HPCE)
 λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0; 2-mercaptoethanol
75784 Atto 565 ≥75% (sum of isomers, HPCE/HPLC)
 λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
72464 Atto 565 NHS ester ≥70.0% (coupling to amines)
 λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0
18507 Atto 565-maleimide λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
03722 Atto 580 Q ≥98.0% (HPCE)
44756 Atto 580 Q NHS ester ≥90% (coupling to amines)
68152 Atto 580 Q maleimide ≥98.0% (HPCE)
70425 Atto 590 ≥90% (HPCE)
 λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
79636 Atto 590 NHS ester ≥60% (coupling to amines)
 λex 590 nm; λem ~620 nm in 0.1 M phosphate pH 7.0
39887 Atto 590-maleimide
08637 Atto 594 ≥98.0% (HPCE)
 λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
08741 Atto 594 NHS ester ≥80% (coupling to amines)
 λex 597 nm; λem 625 nm in 0.1 M phosphate pH 7.0
08717 Atto 594 maleimide ≥80% (coupling rate)
 λex 601 nm; λem 627 nm in 0.1 M phosphate pH 7.0
78493 Atto 610 ≥85.0% (HPCE/HPLC)
 λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
93259 Atto 610 N-succinimidyl ester ≥90.0% (coupling to amines)
 λex 610 nm; λem 635 nm in 0.1 M phosphate pH 7.0
41061 Atto 610-maleimide ≥98% (HPCE)
 λex 610 nm; λem 637 nm in 0.1 M phosphate pH 7.0; 2-mercaptoethanol
40049 Atto 611X λex 611 nm; λem 673 nm in 0.1 M phosphate pH 7.0
18708 Atto 611X NHS ester ≥90% (coupling to amines)
 λex 611 nm; λem 673 nm in 0.1 M phosphate pH 7.0
04327 Atto 612 Q ≥98.0% (HPCE)
53988 Atto 612 Q NHS ester ≥90% (coupling to amines)
50332 Atto 612 Q maleimide ≥98.0% (HPLC)
92716 Atto 620 ≥90% (HPCE)
 λex 617 nm; λem 641 nm in 0.1 M phosphate pH 7.0
67351 Atto 620 NHS ester ≥80% (coupling to amines)
 λex 620 nm; λem 641 nm in 0.1 M phosphate pH 7.0
18620 Atto 633
01464 Atto 633 NHS ester ≥90.0% (coupling to amines)
 λex 630 nm; λem 650 nm in 0.1 M phosphate pH 7.0
08968 Atto 635 ≥90% (HPCE)
 λex 635 nm; λem 656 nm in 0.1 M phosphate pH 7.0
43429 Atto 635 NHS ester ≥80% (coupling to amines)
 λex 624 nm; λem 655 nm in 0.1 M phosphate pH 7.0
97875 Atto 647 ≥90% (HPCE)
 λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0
07376 Atto 647 NHS ester ≥80% (coupling to amines)
 λex 643 nm; λem 669 nm in 0.1 M phosphate pH 7.0
41784 Atto 647 maleimide ≥90% (coupling to thiols)
 λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0
04507 Atto 647N ≥98.0% (HPCE)
 λex 644 nm; λem 667 nm in 0.1 M phosphate pH 7.0
18373 Atto 647N NHS ester ≥85% (coupling to amines)
 λex 647 nm; λem 661 nm in 0.1 M phosphate pH 7.0
05316 Atto 647N maleimide ≥90% (sum of isomers, HPCE)
 λex 644 nm; λem 669 nm in 0.1 M phosphate pH 7.0
93711 Atto 655 ≥85% (HPLC)
 λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
76245 Atto 655 NHS ester ≥90.0% (HPCE)
 λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
80661 Atto 655-maleimide ≥90% (coupling rate)
 λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0