Sigma-Aldrich
Product Catalog →  Biochemicals and Reagents →  Fluorescent Probes, Labels, Particles and Stains →  Fluorescent Labels →  Fluorescein and Derivatives
 Print Preview

Fluorescein and Derivatives

Fluorescein fluorophores have been the most common fluorescent derivatization reagents for covalently labeling proteins. Fluorescein isothiocyanates (FITC) are the predominantly used fluorescein derivatives. But alternative derivatives offer significant advantages. Carboxyfluorescein succinimidyl esters react faster than FITC and yield conjugates with higher resistance against hydrolysis. An advantage of fluorescein dichlorotriazine (DTAF) is its direct reactivity with polysaccharides and other alcohols in aqueous solutions at pH greater than nine. 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein (JOE) is very common as label for automated nucleic acid sequencing. The synthesis of these derivatives typically results in a mixture of isomers, which are almost indistinguishable in both wavelength and intensity, but may differ in the geometry of their binding to proteins. Certain applications may require the single-isomer preparations.

Product #
Description
Assay
Fluorescence
07217 1-(O′-Methylfluoresceinyl)piperidine-4-carboxylic acid ≥95.0% (HPCE)
35845 2′,7′-Dichlorofluorescein diacetate ≥95% (HPCE)
 λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)
444839 4(5)-(Iodoacetamido)fluorescein
C7153 5(6)-Carboxyfluorescein Mixed isomers ~99% (HPLC)
C8166 5(6)-Carboxyfluorescein diacetate Mixed isomers ≥90% (HPLC)
21888 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester ≥90% (HPLC)
 λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
36565 5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein hydrochloride ≥99.0% (TLC)
 λex 492 nm; λem 514 nm in 0.1 M Tris pH 9.0
72755 5-(Bromomethyl)fluorescein ≥95% (sum of tautomers, GC)
 λex 492 nm; λem 515 nm in 0.1 M Tris pH 9.0
I9271 5-(Iodoacetamido)fluorescein ≥95% (HPLC)
D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride ≥90% (HPLC)
 λex 495 nm; λem 516 nm
87444 5-Carboxy-fluorescein diacetate N-succinimidyl ester ≥95.0% (HPLC)
86826 5-Carboxyfluorescein ≥95% (HPLC)
 λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
92846 5-Carboxyfluorescein N-succinimidyl ester ≥90% (HPLC)
 λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0
C4109 6-Carboxy-4′,5′-dichloro-2',7'-dimethoxyfluorescein N-hydroxysuccinimide ester
08951 6-Carboxy-fluorescein diacetate N-succinimidyl ester ≥90.0% (HPLC)
 λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)
C0662 6-Carboxyfluorescein ~97% (HPLC)
54115 6-Carboxyfluorescein ≥95% (HPLC)
 λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
596078 6-Carboxyfluorescein
52352 6-Carboxyfluorescein N-hydroxysuccinimide ester ≥85% (HPLC)
≥90% (coupling to amines)
 λex 492 nm; λem 516 nm in 0.1 M Tris pH 8.0
46935 6-[Fluorescein-5(6)-carboxamido]hexanoic acid ≥90% (HPCE)
 λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0
46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester ≥75% (HPLC)
 λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0
A1731 Aminophenyl fluorescein λex 490 nm; λem 515 nm
D6693 Diaminofluorescein-FM diacetate λex 500 nm; λem 515 nm in DMSO
E6003 Eosin Y disodium salt
E4382 Eosin Y disodium salt
32617 Eosin Y disodium salt
45240 Eosin Y disodium salt
318906 Eosin Y solution
F2456 Fluorescein (free acid)
F3651 Fluorescein 5(6)-isothiocyanate ~90% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate ≥90% (HPLC)
 λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
46939 Fluorescein diacetate 5-maleimide ≥98% (HPCE)
 λex 492 nm; λem 530 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol and after cleavage by esterase)
36438 Fluorescein diacetate 6-isothiocyanate ≥90% (HPLC)
 λex 496 nm; λem 517 nm in 0.1 M Tris pH 8.0, esterase
F7250 Fluorescein isothiocyanate isomer I ≥90% (HPLC)
 λex 492 nm; λem 518 nm (green)
F4274 Fluorescein isothiocyanate isomer I ~98% (HPLC)
 λex 492 nm; λem 518 nm (green)
46951 Fluorescein isothiocyanate isomer I ≥97.5% (HPLC)
 λex 492 nm; λem 518 nm (green)
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
46952 Fluorescein isothiocyanate isomer I ≥90% (HPLC)
 λex 492 nm; λem 518 nm (green)
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
F2502 Fluorescein isothiocyanate isomer I 90%
 λex 492 nm; λem 518 nm (green)
46980 Fluorescein mercuric acetate ~90% (UV)
28803 Fluorescein sodium salt 98.5-100.5% (calc. on dry substance)
46970 Fluorescein sodium salt
F9551 Fluorescein-5-EX N-hydroxysuccinimide ester
88596 Fluorescein-O′-acetic acid ≥90% (HPCE)
 λex 455 nm; λem 514 nm in 0.1 M Tris pH 8.0
H7289 Hydroxyphenyl fluorescein λex 490 nm; λem 515 nm
07294 O′-(Carboxymethyl)fluoresceinamide ≥97.0% (HPCE)
 λex 458 nm; λem 520 nm in methanol

Use of this web site constitutes your acceptance of the Site Use Terms

privacy | terms and conditions of sale | Business Development

 

© 2008 Sigma-Aldrich Co. Reproduction forbidden without permission.
Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc. Best viewed in IE6 or higher