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Mega Labels

In contrast to most common fluorescent labels Mega labels are characterized by a large gap between excitation and emission wavelength. This property minimizes background fluorescence resulting from scattered excitation light or autofluorescence. It also enables parallel visualization or measurement of different targets in one sample without any variation of excitation light. Mega labels can be excited with argon lasers or other widely used light sources in the same manner, but the individual fluorescence of each Megal label varies. Mega labels, if combined with Atto labels, enable the visualization of different structures with just one excitation source. Such label combinations can be used with fluorescence microscopes, using a mercury lamp and standard filter sets optimized for conventional fluorophores. The small size of these labels and the direct conversion of excitation light into emission without the use of a FRET mechanism are crucial advantages for applications like fluorescence microscopy and fluorescence in situ hybridization.

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Product #	Description	Assay	Fluorescence
41087	Fluorescent Red Mega 480	≥90% (HPLC)	λ_ex 526 nm; λ_em 640 nm in 0.1 M phosphate pH 7.0
55536	Fluorescent Red Mega 480 NHS-ester	≥90% (coupling to amines)	λ_ex 513 nm; λ_em 640 nm in 0.1 M phosphate pH 7.0
30487	Fluorescent Red Mega 485	≥85% (HPLC)	λ_ex 482 nm; λ_em 559 nm in 0.1 M phosphate pH 7.0
68360	Fluorescent Red Mega 485 NHS-ester	≥90% (coupling to amines)	λ_ex 482 nm; λ_em 559 nm in 0.1 M phosphate pH 7.0
13194	Fluorescent Red Mega 490	≥98.0% (HPLC)	λ_ex 405 nm; λ_em 541 nm in 0.1 M phosphate pH 7.0
30543	Fluorescent Red Mega 490 NHS-ester	≥65% (coupling to amines)	λ_ex 387 nm; λ_em 540 nm in 0.1 M phosphate pH 7.0
56900	Fluorescent Red Mega 500	≥90% (HPLC)	λ_ex 508 nm; λ_em 608 nm in 0.1 M phosphate pH 7.0
08087	Fluorescent Red Mega 500 NHS-ester	≥70% (coupling to amines)	λ_ex 508 nm; λ_em 612 nm in 0.1 M phosphate pH 7.0
41306	Fluorescent Red Mega 520	≥97.0% (HPLC)	λ_ex 527 nm; λ_em 663 nm in 0.1 M phosphate pH 7.0
68493	Fluorescent Red Mega 520 NHS-ester	≥90% (coupling to amines)	λ_ex 556 nm; λ_em 667 nm in 0.1 M phosphate pH 7.0

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