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Phycobili Proteins

Phycobili Proteins - Enhanced Sensitivity
Sigma offers activated, biotinylated or streptavidin linked phycoerythrin, phycocyanin, and CryptoFluor™ dyes. These dyes are the first choice for applications that require either high sensitivity or simultaneous multicolor detection, e.g. flow cytometry and immunoassay. Phycobili proteins exhibit both quantum yields near 100% and extremely high extinction coefficients. The fluorescence yield of a single molecule is equivalent to at least 30 fluorescein or 100 rhodamine molecules at comparable wavelengths. CryptoFluor dyes are phycobiliproteins isolated from cryptomonad algae that provide additional spectral characteristics to complement the more common phycobiliproteins (e.g., R-PE and APC) as well as providing much smaller molecular weights.
CryptoFluor™ is a trademark of Martek Biosciences Corporation, Columbia, MD, USA.

Product #
Description
Assay
Fluorescence
23192 Allophycocyanin λex 604 nm; λem 661 nm in 0.1 M phosphate pH 7.2
07367 B-Phycoerythrin λex 542 nm; λem 576 nm in 0.1 M phosphate pH 7.2
44709 B-Phycoerythrin, activated λex 542 nm; λem 576 nm in 0.1 M phosphate pH 7.2
17861 Biotin-CryptoFluor™ Crimson λex 585 nm; λem ~658 nm in 0.1 M phosphate pH 7.2
75166 Biotin-B-Phycoerythrin λex 542 nm; λem 576 nm in 0.1 M phosphate pH 7.0
52468 C-Phycocyanin λex 609 nm; λem 643 nm in 0.1 M phosphate pH 7.2
43395 C-Phycocyanin, activated λex 600 nm; λem 641 nm in 0.1 M phosphate pH 7.0
52412 R-Phycoerythrin λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.2
P8912 R-Phycoerythrin from Porphyra tenera “Nori” λex 488 nm; λem 578 nm(lit.)
92984 Streptavidin B-Phycoerythrin λex 542 nm; λem 575 nm in 0.1 M phosphate pH 7.0
61707 Streptavidin C-Phycocyanin λex 600 nm; λem 640 nm in 0.1 M phosphate pH 7.2
17869 Streptavidin−C-Phycocyanin λex 600 nm; λem 640 nm in 0.1 M phosphate pH 7.2
17866 Streptavidin−CryptoFluor™ Crimson λex 585 nm; λem ~658 nm in 0.1 M phosphate pH 7.2
17867 Streptavidin-CryptoFluor™ Gold λex 566 nm; λem ~600 nm in 0.1 M phosphate pH 7.2

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