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Rhodamine and Derivatives

Rhodamines are supplements to fluoresceins, as they offer longer wavelength emission maxima and provide opportunities for multicolor labeling or staining. DNA sequencing, microarray detection and fluorescence in situ hybridization (FISH) demand a number of fluorophores with distinct spectra. Rhodamines exhibit higher photostability than fluorescein. Isothiocyanates and succinimidyl esters are available for convenient labeling.

Tetramethylrhodamine (TMR): a common fluorophore for preparing protein conjugates, especially antibody and avidin conjugates
Carboxy tetramethyl-rhodamine (TAMRA): important for olignucleotide labeling and automated nucleic acid sequencing
Carboxy-X-rhodamine (ROX): important for oligonucleotide labeling and automated nucleic acid sequencing
Sulforhodamine 101: a common protein label for three-color applications

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Product #	Description	Assay	Fluorescence
21953	5(6)-Carboxytetramethylrhodamine	≥85% (sum of isomeres, HPCE)	λ_ex 543 nm; λ_em 570 nm in methanol
C4759	5(6)-Carboxytetramethylrhodamine N-hydroxysuccinimide ester	~90%
21955	5(6)-Carboxytetramethylrhodamine N-succinimidyl ester	~80% (HPLC)	λ_ex 543 nm; λ_em 576 nm in methanol λ_ex 554 nm; λ_em 584 nm in 0.1 M phosphate pH 8.0
21965	5(6)-Carboxy-X-rhodamine		λ_ex 570 nm; λ_em ~595 nm in 0.1 M Tris pH 8.0
21966	5(6)-Carboxy-X-rhodamine N-succinimidyl ester		λ_ex 575 nm; λ_em 600 nm in 0.1 M phosphate pH 7.0
53048	5-Carboxy-tetramethylrhodamine N-succinimidyl ester		λ_ex 543 nm; λ_em 578 nm in 0.1 M phosphate pH 7.0
41969	5-Carboxy-X-rhodamine N-succinimidyl ester		λ_ex 575 nm; λ_em 600 nm in 0.1 M phosphate pH 7.0
53047	5-Carboxy-X-rhodamine triethylamine salt	≥90% (HPLC)	λ_ex 575 nm; λ_em 600 nm in 0.1 M Tris pH 8.0
C2734	5-Carboxytetramethylrhodamine		λ_ex ~543 nm; λ_em ~570 nm in methanol
22575	5-Carboxytetramethylrhodamine	≥95% (HPLC)	λ_ex 543 nm; λ_em 575 nm in 0.1 M Tris pH 8.0 λ_ex ~543 nm; λ_em ~570 nm in methanol
88997	6-Carboxy-tetramethylrhodamine N-succinimidyl ester		λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0
C3734	6-Carboxy-X-rhodamine
52472	6-Carboxy-X-rhodamine	≥90% (HPLC)	λ_ex 575 nm; λ_em 600 nm in 0.1 M Tris pH 8.0
44617	6-Carboxy-X-rhodamine N-succinimidyl ester
55222	6-Carboxytetramethylrhodamine	≥90% (HPLC)	λ_ex 543 nm; λ_em 575 nm in 0.1 M Tris pH 8.0
D6568	Diaminorhodamine-4M		λ_ex 560 nm; λ_em 575 nm in DMSO
D6443	Diaminorhodamine-4M AM		λ_ex 560 nm; λ_em 575 nm in DMSO
05803	N-(2-Aminoethyl)rhodamine 6G-amide bis(trifluoroacetate)	≥95.0% (HPCE)	λ_ex 528 nm; λ_em 553 nm in 0.1 M phosphate pH 3.0
00814	N-(6-Aminohexyl)rhodamine 6G-amide bis(trifluoroacetate)	≥95.0% (HPCE)	λ_ex 528 nm; λ_em 553 nm in 0.1 M phosphate pH 3.0
42514	N-(8-Amino-3,6-dioxaoctyl)rhodamine 6G-amide bis(trifluoroacetate)	≥95.0% (HPCE)	λ_ex 528 nm; λ_em 553 nm in 0.1 M phosphate pH 3.0
29297	N-[2-(2-Aminoethylamino)ethyl]rhodamine 6G-amide bis(trifluoroacetate)	≥90% (HPCE)	λ_ex 528 nm; λ_em 553 nm in 0.1 M phosphate pH 3.0
42932	N-[4-(Aminomethyl)benzyl]rhodamine 6G-amide bis(trifluoroacetate)	≥95.0% (HPCE)	λ_ex 528 nm; λ_em 553 nm in 0.1 M phosphate pH 3.0
90877	N-[trans-4-(Succinimidyloxycarbonyl)cyclohexylmethyl]-sulforhodamine B-acid amide	≥85.0% (coupling to amines)	λ_ex ≥566 nm; λ_em ≥587 nm in 0.1 M phosphate pH 7.0
77418	Phalloidin – tetramethylrhodamine B isothiocyanate conjugate from Amanita phalloides
83686	Rhodamine B 5-[3(4)-(aminocarbonyl)-4(3)-carboxybenzenesulfonyl fluoride]	≥98.0% (¹⁹F-NMR)
283924	Rhodamine B isothiocyanate
R1755	Rhodamine B isothiocyanate
83692	Rhodamine B isothiocyanate		λ_ex 540 nm; λ_em 573 nm in ethanol
S3388	Sulforhodamine 101 acid chloride
86185	Sulforhodamine 101 acid chloride		λ_ex 586 nm; λ_em 605 nm in H₂O
86187	Sulforhodamine B 2-acid fluoride	≥97.0% (UV)	λ_ex 560 nm; λ_em ~580 nm in chloroform
86188	Sulforhodamine B 5-acid fluoride	~85% (UV)
86186	Sulforhodamine B acid chloride		λ_ex 560 nm; λ_em 580 nm in chloroform λ_ex 560 nm; λ_em ~580 nm in chloroform
86190	Sulforhodamine Q 2-acid fluoride	≥97.0% (UV)
T2639	Tetramethylrhodamine isothiocyanate mixed isomers
87918	Tetramethylrhodamine isothiocyanate mixed isomers		λ_ex 529 nm; λ_em 596 nm in DMSO
94506	Tetramethylrhodamine-5-maleimide	≥85% (HPLC)	λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.0
92364	Tetramethylrhodamine-6-maleimide	≥50% (HPLC) ≥97% (isomer purity, HPLC)	λ_ex 543 nm; λ_em 575 nm in 0.1 M phosphate pH 7.5

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