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Biochemical Reagents for Identification

A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria.
Classical biochemical tests are often used to identify microorganisms; the results are seen by color change. In most cases, detection is based on the reaction of an enzyme with a certain substrate. Also, methods to detect certain metabolites by chemical reaction and or complex building techniques are used. At the end, a color change gives a result that leads to greater cognition of the unknown organism.

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Product #	Description	Synonym	Application
29333	Barritt’s Reagent A BioChemika, for microbiology	Voges-Proskauer Test with Barritt’s Reagent A VP1 Reagent	This reagent is used in Voges-Proskauer test for detection of acetoin production by bacterial cultures along with Barritt Reagent B.
39442	Barritt’s Reagent B BioChemika, for microbiology	Voges-Proskauer Test with Barritt’s Reagent B VP2 Reagent	This reagent is used in Voges-Proskauer test for detection of acetoin production by bacterial cultures along with Barritt Reagent A.
07770	N,N-Dimethyl-p-phenylenediamine dihydrochloride puriss. p.a., ≥99.0% (AT)	4-Amino-N,N-dimethylaniline dihydrochloride DMPD · 2HCl DMPPDA · 2HCl	Test reagent in microbiology for oxidase-positive aerobic microorganisms.
49825	DMACA Reagent BioChemika, for microbiology	4-(Dimethylamino)-cinnamaldehyde solution	Used to determine the ability of an organism to split indole from the tryptophan molecule.
03891	Ehrlich’s solution BioChemika	4-(Dimethylamino)benzaldehyde – hydrochloric acid solution	reagent for urobilinogen
88597	Hydrogen peroxide solution BioChemika, for microbiology, 3%	Catalase Test
60983	Kovac’s reagent for indoles BioChemika, for microbiology	4-(Dimethylamino)benzaldehyde solution Indole reagent according to Kovac	In the presence of oxygen, some bacteria, like E. coli, are able to split tryptophan into indole and α-aminopropionic acid. This reagent is for detecting the indole and identifying the indole-positive and indole-negative microorganisms.
08714	Methyl Red solution BioChemika, for microbiology		Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red.
39441	Nitrate Reagent B BioChemika, for microbiology	4-Aminobenzenesulfonic acid Sulfanilic acid solution	α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.
07689	O’Meara’s Reagent BioChemika, for microbiology	Voges-Proskauer test with O’Meara Reagent	The reagent is used in Voges-Proskauer test for the detection of acetoin production by bacterial cultures.
07345	Oxidase Reagent according to Gaby-Hadley A BioChemika, for microbiology	Gaby-Hadley Reagent A	The reagent is used for detection of oxidase activity of bacterial culture along with Oxidase Reagent B according to Gaby-Hadley.
07817	Oxidase Reagent according to Gaby-Hadley B BioChemika, for microbiology	Gaby-Hadley Reagent B	The reagent is used for detection of oxidase activity of a bacterial culture along with Oxidase Reagent A according Gaby-Hadley.
18502	Oxidase Reagent according to Gordon-McLeod BioChemika, for microbiology	Gordon-McLeod Reagent	The reagent is used for detection of oxidase activity of a bacterial culture.
80353	TDA Reagent BioChemika, for microbiology	Ferric chloride solution Iron(III) chloride solution Tryptophan Deaminase Reagent	For identification of Proteus species by detection of tryptophan deaminase activity.

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