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Chromogenic Media

Culture media for the simple and fast detection of bacteria using chromogenic substrates. The chromogenic mixture contains chromogenic substrates as Salmon-GAL, X-Gal, X-glucuronide, etc. Certain enzymes, produced by some bacteria, cleave these substrates, resulting in the different coloration of certain bacteria colonies.

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Product #	Description	Synonym	Application
72953	Candida Ident Agar BioChemika, for microbiology		For the selective isolation and identification of Candida albicans from clinical material like stool, urine, skin scurf and swabs. In addition the Medium is also used for the isolation and identification of a wide range of Gonnococci, Yeasts and molds.
94382	Candida Ident Agar, modified BioChemika, for microbiology		For the selective isolation and identification of Candida species from clinical material like stool, urine, skin scurf and swabs.
12398	CP ChromoSelect Agar BioChemika, for microbiology	Clostridium perfringens ChromoSelect Agar Clostridium perfringens Chromogen Agar CPC Agar
05662	HiCrome^™ Aureus Agar Base BioChemika, for microbiology	Staphylococcus aureus Agar HiCrome^™ Aureus Agar Base HiCrome^™	HiCrome Aureus Agar Base is recommended for the isolation and enumeration of coagulase positive Staphylococcus aureus from environment samples. Coagulase positive S. aureus gives brown-black colonies where as S. epidermidis gives yellow-slightly brownish colonies.
92325	HiCrome^™ Bacillus Agar BioChemika, for microbiology	Bacillus cereus HiCrome^™ Agar	HiCrome^™ Bacillus Agar is a differential medium recommended for rapid identification of Bacillus species from a mixed culture by chromogenic method.
81938	HiCrome^™ Coliform Agar BioChemika, for microbiology	Coliform Agar HiCrome^™	HiCrome^™ Coliform Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples. Sodium lauryl sulfate inhibits Gram-positive organisms. The chromogenic mixture contains two chromogenic substrates, Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction, thereby increasing detection reliability in combination with the two chromogens. To confirm E. coli, add a drop of Kovac's reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
73009	HiCrome^™ ECC Agar BioChemika	E. coli Coliform Agar HiCrome^™ ECC Agar HiCrome^™	HiCrome ECC Agar is a differential medium recommended for the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. The chromogenic mixture containstwo chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme beta glucuronidase produced by Escherichia coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by majority of coliforns, including Escherichia coli. Color of E. coli colonies: blue/purple
85927	HiCrome^™ ECC Selective Agar BioChemika, for microbiology	E. coli-Coliform Selective Agar HiCrome^™ ECC Selective Agar HiCrome^™	HiCrome^™ ECC Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac's reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.
09142	HiCrome^™ ECD Agar with MUG BioChemika, for microbiology	ECD Agar with MUG, HiCrome^™	For the detection of Escherichia coli in water and food samples by using a combination of chromogenic and fluorogenic substrate.
39894	HiCrome^™ EC O157 Agar BioChemika, for microbiology	EC O157 Agar HiCrome^™	HiCrome EC O157 Agar is a chromogenic medium for isolation and differentiation of E. coli O157 from food and environmental samples.
72557	HiCrome^™ EC O157:H7 Selective Agar, Basis BioChemika, for microbiology	EC O157:H7 Selektive Agar HiCrome^™, Base	HiCrome EC O157:H7 Selective Agar is recommended for selective isolation and easy detection of Escherichia coli O157:H7 from food samples.
95207	HiCrome^™ E. coli Agar A BioChemika, for microbiology	E. coli Agar A HiCrome^™	HiCrome^™ Escherichia coli Agar A is recommended for the detection and enumeration of E. coli in foods without further confirmation on membrane filter or by indole reagent. Most of the E. coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for E. coli. The chromogenic agent X-glucuronide used in this medium helps to detect glucuronidase activity. E. coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies. Casein enzymic hydrolysate, peptone special provide the essential growth nutrients to the organisms. Bile salts mixture inhibits gram-positive organisms.
70722	HiCrome^™ E. coli Agar B BioChemika	E. coli Agar B HiCrome^™	HiCrome E. coli Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.
80330	HiCrome^™ Enrichment Broth (Base) for EC 0157:H7 BioChemika, for microbiology	EC 0157:H7 HiCrome^™ Enrichment Broth (Base)	HiCrome^™ Enrichment Broth (Base) for EC 0157:H7 is recommended for isolation and selective differentiation of E. coli 0157:H7 from food and environmental samples by chromogenic method.
92324	HiCrome^™ Enterobacter sakazakii Agar BioChemika, for microbiology	Enterobacter sakazakii HiCrome^™ Agar	HiCrome^™ Enterobacter sakazakii Agar is recommended for isolation and identification of Enterobacter sakazakii from food, water, sewage, urine and faeces samples.
14703	HiCrome^™ Enterobacter sakazakii Agar, modified BioChemika, for microbiology	Enterobacter sakazakii HiCrome^™ Agar, modified Enterobacter sakazakii isolation agar ESIA	HiCrome^™ Enterobacter sakazakii Agar modified meets the formulation recommended by ISO Committee for the isolation and identification of Enterobacter sakazakii from milk and milk products.
52441	HiCrome^™ Enterococci Broth BioChemika, for microbiology	Enterococci HiCrome^™ Broth	HiCrome^™ Enterococci Broth is recommended for identification and differentiation of Enterococci from water samples.
90919	HiCrome^™ Enterococcus faecium Agar Base BioChemika, for microbiology	Enterococcus faecium HiCrome^™ Agar Base	HiCrome^™ Enterococcus faecium Agar is recommended for chromogenic identification of Enterococcus faecium from faeces, sewage and water supplies.
90925	HiCrome^™ Klebsiella Selective Agar Base BioChemika, for microbiology	Klebsiella Selective HiCrome^™ Agar Base	HiCrome^™ Klebsiella Selective Agar for selective isolation and easy detection of Klebsiella species from water and other sources. This medium can also be used in membrane filtration procedure.
53707	HiCrome^™ Listeria Agar Base, modified BioChemika, for microbiology	Listeria HiCrome^™ Agar Base, modified	HiCrome^™ Listeria Agar Base, modified is a selective and differential agar medium recommended for rapid and direct identification of Listeria species, specifically Listeria monocytogenes.
83339	HiCrome^™ MacConkey-Sorbitol Agar BioChemika, for microbiology	MacConkey-Sorbitol Agar HiCrome^™ SMAC Agar HiCrome^™ Sorbitol MacConkey Agar HiCrome^™	HiCrome^™ MacConkey Sorbitol Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β-D-glucuronidase which is specific for E. coli. Stranins of E. coli possessing β-D-glucuronidase appear as blue colored colonies on the medium. Enteropathogenic strains of E. coli 0157 do not possess beta-D-glucuronidase activity and thus produce colorless colonies. E. coli fermenting sorbitol and possessing beta-D-glucuronidase activity produce purple colored colonies. Most of the gram-positive organisms are inhibited by crystal violet and bile salts. Addition of Tellurite-Cefixime Supplement makes the medium selective. Potassium Tellurite selects the serogroups 0157 from other E. coli serogroups and inhibits Aeromonas species and Providencia species. Cefixime inhibits Proteus species.
75605	HiCrome^™ M-CP Agar Base BioChemika, for microbiology	M-CP HiCrome^™ Agar Base Membrane Clostridium perfringens HiCrome^™ Agar Base	The m-CP Agar Base with selective supplement is recommended by the Directive of the Council of the European Union 98/83/EC for isolation and enumeratin of Clostridium perfringens from water samples using membrane filtration technique.
90923	HiCrome^™ MeReSa Agar Base BioChemika, for microbiology	HiCrome^™ Methicillin Resistant Staphylococcus aureus Agar Base MRSA Chromogen Agar	HiCrome^™ MeReSa Agar is recommended for the isolation and selective identification of Methicillin Resistant Staphylococcus aureus (MRSA) from clinical isolates.
00563	HiCrome^™ MM Agar BioChemika, for microbiology	Miller and Mallinson Agar, HiCrome^™ MM Agar HiCrome^™	HiCrome MM Agar is recommended for identification and differentiation of Salmonella and non-salmonella like Citrobacter from water samples.
87898	HiCrome^™ MS.O157 Agar BioChemika	MS.O157 Agar HiCrome^™
66481	HiCrome^™ OGYE Agar Base BioChemika, for microbiology	OGYE Agar Base HiCrome^™ Oxytetracyclin Glucose Yeast Extract Agar HiCrome^™	HiCrome OGYE Agar Base is a selective and differential medium which facilitates rapid isolation of yeasts and moulds from milk and milk products¹. Oxytetracycline makes the medium more selective by inhibiting the growth of Lactobacilli. A. niger appears as light blue colored colonies with black spores due to presence of chromogenic mixture, C. albicans shows green colored colonies and S. cerevisiae gives colorless colonies.
90918	HiCrome^™ RajHans Medium, modified BioChemika, for microbiology	HiCrome^™ Salmonella Medium, modified Salmonella RajHans Chromogen Medium, modified	HiCrome^™ RajHans Medium modified is recommended for identification and differentiation of Salmonella species from the members of Enterobacteriaceae, especially Proteus species.
51489	HiCrome^™ Rapid Coliform Broth BioChemika, for microbiology	Coliform Rapid HiCrome^™ Broth Rapid Coliform HiCrome^™ Broth	Rapid HiColiform Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.
51759	HiCrome^™ Rapid Enterococci Agar BioChemika, for microbiology	Enterococci Rapid HiCrome^™ Agar Rapid Enterococci HiCrome^™ Agar	HiCrome^™ Rapid Enterococci Agar is used for rapid and easy identification and differentiation of Enterococci from water samples. It contains chromogenic substrate, which aids in the detection of Enterococci from water samples.
78419	HiCrome^™ Salmonella Agar BioChemika	Salmonella Agar HiCrome^™	HiCrome Salmonella Agar is a selective medium used for simultaneous detection of Escherichia coli and Salmonella from food and water. Escherichia coli and Salmonella are easily distinguishable due to the colony characteristics. Salmonella give light purple colonies a halo. Escherichia coli has a characteristic blue color, other organisms give colorless colonies. The characteristic light purple and blue color is due to the chromogenic mixture.
05538	HiCrome^™ Salmonella Agar, Improved BioChemika, for microbiology	Salmonella Agar HiCrome^™, Improved	A selective chromogenic medium used for simultaneous detection of Salmonella and Escherichia coli from food and water samples.
90924	HiCrome^™ M-TEC Agar BioChemika, for microbiology	M-TEC HiCrome^™ Agar	HiCrome^™ M-TEC Agar is recommended by the U.S. Environmental Protection Agency (USEPA) for differentiation and enumeration of thermotolerant E.coli from water by membrane filtration technique.
16636	HiCrome^™ UTI Agar, modified BioChemika, for microbiology	Urinary Tract Infection Agar, modified UTI HiCrome^™ Agar, modified	HiCrome^™ UTI Agar is chromogenic differential medium for identification, differentiation, and conformation of enteric bacteria from specimens such as urine, water, or food which may contain large number of Proteus species as well as potentially pathogenic Gram-positive organisms. Based on these characteristics, Modified HiCrome UTI Agar is suggested for use in place of MacConkey Agar.
92323	HiCrome^™ Vibrio Agar BioChemika, for microbiology	Vibrio HiCrome^™ Agar	HiCrome^™ Vibrio Agar is recommended for isolation, and selective chromogenic differentiation of Vibrio species from seafood.
77408	Listeria mono Differential Agar (Base) BioChemika, for microbiology	Agar Listeria Ottaviani & Agosti Listeria monocytogenes Differential Agar (Base)	L. mono differential Agar has been recommended by ISO Committee for the selective and differential isolation of Listeria monocytogenes.
39734	Membrane Lactose Glucuronide Agar BioChemika, for microbiology	M-Lauryl Sulfate Chromogen Agar MLGA	A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique for E coli and coliforms by reducing the number of filtration stages required from two to one and by reducing the need for further confirmation steps. This medium is described in the Environment Agency's report of UK, 'Methods for Examination of Waters and Associated Material - The Microbiology of Drinking Water (2002) - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7)'.
68879	Phenolphthalein phosphate agar BioChemika, for microbiology		Recommended for identification of phosphatase positive colonies of Staphylococcus aureus.
84369	Salmonella Chromogen Agar BioChemika, for microbiology		A differential diagnostic agar for the detection of Salmonella in food and clinical material. A modification of the formulation described by Rambach (1990).
01993	Salmonella Chromogen Agar Set BioChemika, for microbiology		A differential diagnostic agar for the detection of Salmonella in food and clinical material.
92435	TBX Agar BioChemika, for microbiology	Tryptone Bile X-glucuronide Agar	Chromogenic Agar for the detection and enumeration of E. coli in foodstuffs, animal food and water without further confirmation. E. coli colonies are colored blue-green.

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