Order Center

Fluorogenic Media

Culture media for the simple and fast detection of E. coli or other organisms using fluorescence substrates. E. coli, for example, can be detected using the MUG (glucuronidase) test and confirmed with a positive indole test. ß-D-glucoronidase, which is produced by E. coli, cleaves 4-Methylumbelliferyl-ß-D-glucuronide to 4-methylumbelliferone and glucuronide. The fluorogen 4-methylumbelliferone can be detected under a long wavelength UV lamp.

Reset Table

Product #	Description	Synonym	Application
16016	BRILA MUG Broth BioChemika, for microbiology		In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation. The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.
31401	DEV Lactose Peptone MUG Broth BioChemika, for microbiology		For the enrichment and titre determination of coliform bacteria in connection with the bacteriological examination of water. The presence of E. coli can be demonstrated by fluorescence in the UV and a positive indole test.
44657	ECD MUG Agar BioChemika, for microbiology		The bile-salt mixture in this E. coli Direct Agar extensively inhibits the non-obligatory intestinal accompanying flora. Fluorescence in the UV and a positive indole test demonstrate the presence of E. coli in the colonies.
44782	E. coli O157:H7 MUG Agar BioChemika, for microbiology		Selective agar for the isolation and differentiation of enterohaemorrhagic (EHEC) E. coli O157:H7-strains from food and clinical material.
09142	HiCrome^™ ECD Agar with MUG BioChemika, for microbiology	ECD Agar with MUG, HiCrome^™	For the detection of Escherichia coli in water and food samples by using a combination of chromogenic and fluorogenic substrate.
51489	HiCrome^™ Rapid Coliform Broth BioChemika, for microbiology	Coliform Rapid HiCrome^™ Broth Rapid Coliform HiCrome^™ Broth	Rapid HiColiform Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.
78996	HiFluoro^™ Pseudomonas Agar Base BioChemika, for microbiology	Pseudomonas Agar Base HiFluoro^™	Hifluoro Pseudomonas Agar base is used as a selective medium for the isolation of Pseudomonas aeruginosa from pus, sputum, and drains etc. Pseudomonas aeruginosa breaks the fluorogenic compound to release the fluorogen which produces a visible fluorescence under long wave UV light.
62634	LST-MUG Broth BioChemika, for microbiology		Fluorescent method for the detection of E. coli.
63014	MacConkey MUG Agar BioChemika, for microbiology		For the isolation of Salmonella, Shigella and coliform bacteria, in particular E. coli, from diverse material. Bile salts and crystal violet extensively inhibit the Gram-positive flora. The presence of lactose and neutral red indicate lactose-positive colonies from which E. coli can be identified by fluorescence in the UV.
M1678	MUG EC broth BioChemika, for microbiology	4-Methylumbelliferyl β-D-Glucuronide Escherichia coli Broth	Used for the detection of E. coli by a fluorogenic procedure.
17165	MUG Tryptone Soya Agar BioChemika, for microbiology	CASO MUG Agar Soybean Casein digest MUG Agar Tryptone Soya MUG Agar	For cultivation of fastidious and nonfastidious microorganisms by fluorogenic method.
51413	Plate Count MUG Agar BioChemika, for microbiology	MUG PCA MUG Plate Count Agar	Plate Count MUG Agar is used for determination of plate count of microorganisms in milk and other dairy products by fluorogenic method.
95273	VRB MUG Agar BioChemika, for microbiology	Violet Red Bile Lactose MUG Agar	Selective medium for the detection and enumeration of coliform bacteria, in particular E. coli. Gram-positive accompanying flora are extensively inhibited by crystalviolet and bile salts. A color-change to red indicates lactose-positive colonies, within which E. coli can be demonstrated by fluorescence in the UV.

Copyrights © 2008 Sigma-Aldrich Co. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc.