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Protein Stains For Immunoassays

General Features & Benefits
  • Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
  • Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
  • Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
  • Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays
  • Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
  • Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays
  • Fluorescence polarization excitation of fluorochromes
  • Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies

Product #
Description
64958 2-Methoxy-2,4-diphenyl-3(2H)-furanone BioChemika, for fluorescence, ≥98.0% (HPLC)
25455 4-Chloro-7-nitrobenzofurazan BioChemika, for fluorescence, ≥99.0% (HPLC), puriss.
21955 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence, ~80% (HPLC)
21953 5(6)-Carboxytetramethylrhodamine for fluorescence, ≥85% (sum of isomeres, HPCE)
72755 5-(Bromomethyl)fluorescein BioChemika, for fluorescence, ≥95% (sum of tautomers, GC)
53048 5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence
22575 5-Carboxytetramethylrhodamine BioChemika, for fluorescence, ≥95% (HPLC)
07985 6-Aminofluorescein BioChemika, for fluorescence, ~95% (HPLC)
88997 6-Carboxy-tetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence
55222 6-Carboxytetramethylrhodamine BioChemika, for fluorescence, ≥90% (HPLC)
46940 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester BioChemika, for fluorescence, ≥75% (HPLC)
10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt BioChemika, for fluorescence, ≥97.0% (HPLC)
75068 9,10-Anthracenediyl-bis(methylene)dimalonic acid BioChemika, ≥90% (HPCE)
72335 9-(2,2-Dicyanovinyl)julolidine BioChemika, for fluorescence, ≥97.0% (HPLC)
72301 9-(2-Carboxy-2-cyanovinyl)julolidine BioChemika, for fluorescence, ≥97.0% (HPLC)
77810 Atto 520 NHS ester BioChemika, for fluorescence, ≥80.0% (coupling to amines)
70706 Atto 520 BioChemika, for fluorescence, ≥90.0% (HPCE/HPLC)
72464 Atto 565 NHS ester BioChemika, for fluorescence, ≥70.0% (coupling to amines)
75784 Atto 565 BioChemika, for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
79636 Atto 590 NHS ester BioChemika, for fluorescence, ≥60% (coupling to amines)
70425 Atto 590 BioChemika, for fluorescence, ≥90% (HPCE)
93259 Atto 610 N-succinimidyl ester BioChemika, for fluorescence, ≥90.0% (coupling to amines)
78493 Atto 610 BioChemika, for fluorescence, ≥85.0% (HPCE/HPLC)
76245 Atto 655 NHS ester BioChemika, for fluorescence, ≥90.0% (HPCE)
93711 Atto 655 BioChemika, for fluorescence, ≥85% (HPLC)
75999 Atto 680 NHS ester BioChemika, for fluorescence, ≥90.0% (coupling to amines)
94875 Atto 680 BioChemika, for fluorescence, ≥90% (HPLC)
46950 Fluorescein 5(6)-isothiocyanate BioChemika, for fluorescence, mixture of 2 components, ≥90% (HPLC)
78361 Fluorescent red 630 reactive BioChemika, for fluorescence, ≥80% (coupling to amines)
55817 Fluorescent red 630 BioChemika, ≥85% (HPLC)
39779 Fluorescent red 635 reactive BioChemika, for fluorescence
62164 Fluorescent red 646 reactive BioChemika, for fluorescence
95043 Fluorescent red 646 reactive BioChemika, for fluorescence
47614 Fluram® BioChemika, for fluorescence, ≥99.0% (UV)
62642 Lucifer Yellow CH dilithium salt BioChemika, for fluorescence, ≥85% (HPLC)
62649 Lucifer Yellow CH dipotassium salt BioChemika, for fluorescence
72523 NIR-605-carboxylic acid BioChemika, ~80% (HPCE)
94457 NIR-641 N-succinimidyl ester BioChemika, ≥80% (HPLC)
40741 NIR-641 carboxylic acid BioChemika, ≥90% (HPCE)
91815 NIR-667 N-succinimidyl ester BioChemika
86185 Sulforhodamine 101 acid chloride BioChemika, for fluorescence
86186 Sulforhodamine B acid chloride BioChemika, for fluorescence, technical

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