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Fluorescent Conjugates for Microscopy

Microscopy offers great potential for qualitative and quantitative studies on the structure and function of cells and is uniquely suitable for probing living cells. The specimen is illuminated by light of a certain range of wavelengths, which may not necessarily be monochromatic, while viewing emitted (fluorescence) light of longer wavelengths. New methods, like confocal microscopy, provide better resolution (~1.4 improvement) and enable the measurement of various parameters. A point source and a point detector, both achieved by a pinhole are used, while light from other than the focal plane is defocused at the confocal aperture and thus detected only weakly.


Schematic diagram of a confocal microscope

Product #
Description
17861 Biotin-CryptoFluor Crimson BioChemika, for fluorescence
17864 Biotin-B-Phycoerythrin BioChemika
46947 Fluorescein isothiocyanate – Dextran 500000-Conjugate BioChemika, for fluorescence, (FITC:Glucose = 1:100)
46946 Fluorescein isothiocyanate–dextran BioChemika, for fluorescence, average mol wt 150,000, (FITC:Glucose = 1:160)
46945 Fluorescein isothiocyanate–dextran BioChemika, for fluorescence, average mol wt 70,000, (FITC:Glucose = 1:250)
61761 Lectin-Fluorescein isothiocyanate conjugate from Canavalia ensiformis BioChemika, for fluorescence, lyophilized, powder
77418 Phalloidin – tetramethylrhodamine B isothiocyanate conjugate from Amanita phalloides BioChemika, for fluorescence, mixture of isomers
17866 Streptavidin−CryptoFluor Crimson BioChemika, for fluorescence

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