Fluorescent Conjugates for Immunoassays

General Features & Benefits

Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays

Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays

Fluorescence polarization excitation of fluorochromes
Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies