Order Center
 
Product Catalog →  Biochemicals and Reagents →  Fluorescent Probes, Labels, Particles and Stains →  Fluorescent Labels →  Carbohydrate Labels →  Carbohydrate Labels for Spectroscopy
 Print Preview

Carbohydrate Labels for Spectroscopy

Benefits of Spectroscopy
  • Flexibility regarding excitation and wavelengths as well as sample preparation
  • Relative low cost of instrumentation & outstanding selectivity and sensitivity
  • Identification of biomolecules using multiple parameters
  • Direct detection of ions and determination of pH-values

Steady-State Spectroscopy Most of the applications are based on constant excitation
Conventional Fluorometry - Measurements of emission light intensities at defined wavelengths suggested as excitation certain emission maxima of a fluorophore.
Total Fluorometry - Collection of data for a continuum of absorption as well as emission wavelengths.
Fluorescence Polarization - Polarized light used for excitation. Binding of fluorochrome-labeled antigens to specific antibodies affects polarization extent.
Line Narrowing Spectroscopy - Low-temperature spectroscopy that derives its selectivity from narrow-line emission spectra.

Time-Dependent Fluorescence Spectroscopy Time-resolved measurements contain more information than steady-state measurements, since the steady-state values represent the time average of time-resolved determinations
Single Photon Timing Technique - The time between an excitation light pulse and the first photon emitted by the sample is measured.

Frequency-Domain Fluorescence Spectroscopy The time decay of fluorescence is typically measured using a light source with an intensity modulated sinusoidally at a given frequency, by determining the phase delay and the relative modulation of the fluorescence signal with respect to the exciting light

Product #
Description
Assay
Fluorescence
86181 10-(3-Sulfopropyl)acridinium betaine ≥98.0% (HPLC)
 λex 415 nm; λem 488 nm in H2O
06627 2-Aminoacridone ≥98.0% (HPLC)
 λex 420 nm; λem 538 nm in 0.1 M Tris pH 8.0
54890 2-Phenylphenol ≥98.0% (GC)
P28263 2-Phenylphenol 99%
64785 4-Methoxybenzamidine ≥97.0% (NT)
 λex 393 nm; λem 478 nm (after derivatization with glucose)
λex 393 nm; λem 478 nm(lit.)
08658 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt ≥90% (CE)
 λex 356 nm; λem 512 nm in 0.1 M phosphate pH 7.0
30434 Dansylhydrazine ≥95.0% (HPLC)
 λex 340 nm; λem 520 nm in ethanol

 Site Use Terms | Terms and Conditions of Sale | Privacy |
Copyrights © 2008 Sigma-Aldrich Co. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Sigma-Aldrich brand products are sold exclusively through Sigma-Aldrich, Inc.