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Carbohydrate Labels for Chromatography

Advantages of Fluorescent Derivatization:

Measured light parameters are positive (light signal is zero in absence of fluorophore)
Fluorophoric species are relatively rare in analytical matrices resulting in low background noise levels
Detection problems caused by lack of universal detectors for HPLC can be solved partially by UV and fluorescence derivatization techniques
High sensitivity
Pre-column derivatization offers simplicity and less reagent demand as compared with post-column derivatization
Pre- and post-column derivatization techniques are available for both column chromatography and thin layer chromatography, including reversed-phase chromatography (including ion pair chromatography) and ion exchange chromatography (IEC) systems

Our Glyco Profile^™ Labeling Kits are designed for efficient labeling of N-linked, O-linked and glycosylphosphatidylinositol (GPI) anchored glycans using your choice of 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). See our Glycoproteomics Selection Guide for additional details on labeling, detection and quantitation.

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Product #	Description	Assay	Fluorescence
70684	1-(2-Naphthoyl)imidazole	≥95.0% (N)
70930	1-Naphthaleneacetic anhydride	≥97.0% (T)
438952	1-Naphthaleneacetic anhydride	96%
64958	2-Methoxy-2,4-diphenyl-3(2H)-furanone	≥98.0% (HPLC)	λ_ex 384 nm; λ_em 472 nm in acetonitrile (after derivatization with hexylamine)
66807	4,5-Methylenedioxy-1,2-phenylenediamine dihydrochloride	≥99.0% (HPLC)	λ_ex 367 nm; λ_em 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate)
65877	4-(6-Methyl-2-benzothiazolyl)phenyl isocyanate	≥98.0% (HPLC)	λ_ex 327 nm; λ_em 382 nm in methylene chloride (after derivatization with hexylamine)
25455	4-Chloro-7-nitrobenzofurazan	≥99.0% (HPLC)	λ_ex 420 nm; λ_em ~540 nm in ethanol (after derivatization with glycine)
47140	4-Fluoro-7-nitrobenzofurazan	≥98.0% (HPLC)
53892	4-Hydrazino-7-nitrobenzofurazan	≥95.0% (TLC)	λ_ex 445 nm; λ_em 522 nm in chloroform (after derivatization with formaldehyde)
64785	4-Methoxybenzamidine	≥97.0% (NT)	λ_ex 393 nm; λ_em 478 nm (after derivatization with glucose) λ_ex 393 nm; λ_em 478 nm(lit.)
07985	6-Aminofluorescein	~95% (HPLC)	λ_ex 490 nm; λ_em 520 nm in 0.1 M Tris pH 9.0
36798	7-(Diethylamino)coumarin-3-carbohydrazide	≥95% (HPCE)	λ_ex 450 nm; λ_em 468 nm in methanol
31755	7-(Diethylamino)coumarin-3-carbonyl azide	≥95% (HPLC)	λ_ex 390 nm; λ_em 478 nm in methanol
46640	7-Fluorobenzofurazan-4-sulfonic acid ammonium salt	≥98.5% (HPLC)	λ_ex 385 nm; λ_em 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)
08658	8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt	≥90% (CE)	λ_ex 356 nm; λ_em 512 nm in 0.1 M phosphate pH 7.0
09341	8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt	≥96.0% (HPCE)	λ_ex 420 nm; λ_em 500 nm in 0.1 M Tris pH 7.4 (quenching with Vitamin B₁) λ_ex 488 nm; λ_em 520 nm(lit.)
25102	9-(Chloromethyl)anthracene	≥97.0% (AT)	λ_ex 327 nm; λ_em 412 nm (after derivatization with sodium acetate)
10609	9-Anthracenecarbonyl cyanide	≥98.5% (GC)	λ_ex 361 nm; λ_em 451 nm (after derivatization with ethanol)
10603	9-Anthracenecarboxaldehyde	≥97.0% (HPLC)
10678	Anthranilic acid	≥99.0% (T)	λ_ex 315 nm; λ_em 400 nm(lit.)
10710	Anthranilic acid amide	≥98.0% (NT)
75707	Bis(2,4,6-trichlorophenyl) oxalate	≥99.0% (AT)
75705	Bis(2,4-dinitrophenyl) oxalate	≥97.0% (TLC)
39220	Dansyl chloride	≥99.0% (HPLC)	λ_ex 337 nm; λ_em 492 nm in chloroform (after derivatization with hexylamine)
30434	Dansylhydrazine	≥95.0% (HPLC)	λ_ex 340 nm; λ_em 520 nm in ethanol
42815	Diphenylborinic anhydride	≥95.0% (T)
46985	Fluorescein-5-thiosemicarbazide	~80% (HPCE)	λ_ex 492 nm; λ_em 516 nm in 0.1 M Tris pH 9.0
47614	Fluram^®	≥99.0% (UV)	λ_ex 390 nm; λ_em 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
23184	Fmoc chloride	≥99.0% (HPLC)
PP0530	GlycoProfile^™ 2-AA Labeling Kit
PP0520	GlycoProfile^™ 2-AB Labeling Kit
M8010	N,N′-Dimethyl-9,9′-biacridinium dinitrate
39145	N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide	≥99.0% (HPLC)	λ_ex 398 nm; λ_em 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)
57672	N-(Iodoacetaminoethyl)-1-naphthylamine-5-sulfonic acid	≥95% (HPLC)	λ_ex 337 nm; λ_em 498 nm (after derivatization with 2-mercaptoethanol)
12535	NIR-664-iodoacetamide	≥90% (HPLC)	λ_ex 664 nm; λ_em 689 nm in methanol
79765	Phthaldialdehyde	≥97.0% (HPLC)
79760	Phthaldialdehyde	≥99.0% (HPLC)	λ_ex 340 nm; λ_em 450 nm in reaction buffer; glycine
78411	o-Phenylenediamine
78410	o-Phenylenediamine	≥99.0% (NT)
78446	o-Phenylenediamine dihydrochloride
78441	o-Phenylenediamine dihydrochloride
53897	trans-4′-Hydrazino-2-stilbazole dihydrochloride	≥85% (AT)

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