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Fluorescent Enzyme Substrates for Immunoassays

General Features & Benefits

Immunological methods are based upon the competitive binding of labeled ligands versus unlabeled ones on highly specific receptor sites
Very important tools for clinical and analytical biochemistry and the analysis of drugs, pesticides, hormones and proteins
Outstanding specificity and sensitivity, adaptability to automation & an ability to quantitate an almost limitless number of analytes.
Competitive (higher selectivity) and non-competitive (higher sensitivity) immunoassay design

Heterogeneous Immunoassays

Physical separation of bound from free labeled analyte. Mostly the analyte or antibody is attached to a solid surface
Detection can be direct (only a primary antibody is used) or indirect (a secondary antibody is also used)

Homogenous Immunoassays

Fluorescence polarization excitation of fluorochromes
Double-antibody fluorophore-labeled antigen reaction with antibodies directed against both the antigen and the fluorophore. Labeled and unlabeled antigens compete for a limited number of anti-antigen antibodies

Reset Table

Product #	Description
11557	2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt BioChemika, purum, ≥99.0% (HPLC)
A9941	2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt tablet, 10 mg substrate per tablet
70448	2-Naphthol BioChemika, for analytical purposes, ≥99.0% (GC)
87750	3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate purum, ≥98.0% (calc. on dry substance, AT)
87748	3,3′,5,5′-Tetramethylbenzidine purum, ≥98.0% (NT)
69590	4-Methylumbelliferyl β-D-galactopyranoside BioChemika, for fluorescence, ≥97.0% (HPLC)
71770	4-Nitrophenyl phosphate disodium salt hexahydrate BioChemika, ≥97.0% (NT)
73730	4-Nitrophenyl phosphate disodium salt hexahydrate BioChemika, ≥99.0% (5 mg substrate per tablet, rest binding agent, TLC), tablets
71768	4-Nitrophenyl phosphate disodium salt hexahydrate BioChemika, for enzyme immunoassay, ≥99.0% (enzymatic)
73724	4-Nitrophenyl phosphate disodium salt BioChemika, purum, tablets, 20 mg/tablet substrate (remainder binding agent)
A80602	5-Aminotetrazole monohydrate 97%
09470	5-Aminotetrazole monohydrate purum, ≥96.0% (NT)
37288	Dihydrofluorescein diacetate Standard Fluka, ≥90% (HPLC)
83638	Resorufin β-D-galactopyranoside BioChemika, for fluorescence, ≥95% (HPCE)
93810	Tyramine BioChemika, for fluorescence, ≥99.0% (TLC)
33430	o-Dianisidine purum p.a., for the determination of Au, NO₂^-, Ce(IV) (spectrophotometric), for the detection of Au, Co, Cu, SCN^-, V, ≥97.0% (CHN)
78441	o-Phenylenediamine dihydrochloride BioChemika, for fluorescence, tablet, ~2 mg substrate per tablet
78449	o-Phenylenediamine dihydrochloride BioChemika, tablet, ~30 mg substrate per tablet
P8287	o-Phenylenediamine dihydrochloride tablet, 10 mg substrate per tablet
P6787	o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet
P8412	o-Phenylenediamine dihydrochloride tablet, 30 mg substrate per tablet
P3804	o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
P6912	o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet
78446	o-Phenylenediamine dihydrochloride tablet, ~10 mg substrate per tablet
78411	o-Phenylenediamine BioChemika, for fluorescence, free base
78410	o-Phenylenediamine purum, for fluorescence, ≥99.0% (NT)

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