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Protein Stains for Microscopy

Microscopy offers great potential for qualitative and quantitative studies on the structure and function of cells and is uniquely suitable for probing living cells. The specimen is illuminated by light of a certain range of wavelengths, which may not necessarily be monochromatic, while viewing emitted (fluorescence) light of longer wavelengths. New methods, like confocal microscopy, provide better resolution (~1.4 improvement) and enable the measurement of various parameters. A point source and a point detector, both achieved by a pinhole are used, while light from other than the focal plane is defocused at the confocal aperture and thus detected only weakly.

Schematic diagram of a confocal microscope.

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Product #	Description
21955	5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence, ~80% (HPLC)
21953	5(6)-Carboxytetramethylrhodamine for fluorescence, ≥85% (sum of isomeres, HPCE)
21878	5(6)-Carboxyfluorescein N-hydroxysuccinimide ester BioChemika, for fluorescence, mixture of isomers, ≥90% (HPLC)
72755	5-(Bromomethyl)fluorescein BioChemika, for fluorescence, ≥95% (sum of tautomers, GC)
53048	5-Carboxy-tetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence
22575	5-Carboxytetramethylrhodamine BioChemika, for fluorescence, ≥95% (HPLC)
88997	6-Carboxy-tetramethylrhodamine N-succinimidyl ester BioChemika, for fluorescence
55222	6-Carboxytetramethylrhodamine BioChemika, for fluorescence, ≥90% (HPLC)
08445	7-Amino-4-methyl-3-coumarinylacetic acid BioChemika, for fluorescence, ~90% (HPLC)
10417	8-Anilino-1-naphthalenesulfonic acid ammonium salt BioChemika, for fluorescence, ≥97.0% (HPLC)
10419	8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate BioChemika, for fluorescence, ≥95.0% (T)
75068	9,10-Anthracenediyl-bis(methylene)dimalonic acid BioChemika, ≥90% (HPCE)
72335	9-(2,2-Dicyanovinyl)julolidine BioChemika, for fluorescence, ≥97.0% (HPLC)
72301	9-(2-Carboxy-2-cyanovinyl)julolidine BioChemika, for fluorescence, ≥97.0% (HPLC)
01673	Acriflavine BioChemika, for fluorescence, ≥95.0% (AT)
77810	Atto 520 NHS ester BioChemika, for fluorescence, ≥80.0% (coupling to amines)
70706	Atto 520 BioChemika, for fluorescence, ≥90.0% (HPCE/HPLC)
72464	Atto 565 NHS ester BioChemika, for fluorescence, ≥70.0% (coupling to amines)
75784	Atto 565 BioChemika, for fluorescence, ≥75% (sum of isomers, HPCE/HPLC)
79636	Atto 590 NHS ester BioChemika, for fluorescence, ≥60% (coupling to amines)
70425	Atto 590 BioChemika, for fluorescence, ≥90% (HPCE)
93259	Atto 610 N-succinimidyl ester BioChemika, for fluorescence, ≥90.0% (coupling to amines)
78493	Atto 610 BioChemika, for fluorescence, ≥85.0% (HPCE/HPLC)
76245	Atto 655 NHS ester BioChemika, for fluorescence, ≥90.0% (HPCE)
93711	Atto 655 BioChemika, for fluorescence, ≥85% (HPLC)
75999	Atto 680 NHS ester BioChemika, for fluorescence, ≥90.0% (coupling to amines)
94875	Atto 680 BioChemika, for fluorescence, ≥90% (HPLC)
11340	Auramine O Standard Fluka, for microscopy (Bact., Bot., Fl., Hist.), for the detection of Pb
45242	Eosin yellowish solution BioChemika, for microscopy
78361	Fluorescent red 630 reactive BioChemika, for fluorescence, ≥80% (coupling to amines)
55817	Fluorescent red 630 BioChemika, ≥85% (HPLC)
39779	Fluorescent red 635 reactive BioChemika, for fluorescence
62164	Fluorescent red 646 reactive BioChemika, for fluorescence
95043	Fluorescent red 646 reactive BioChemika, for fluorescence
62642	Lucifer Yellow CH dilithium salt BioChemika, for fluorescence, ≥85% (HPLC)
62649	Lucifer Yellow CH dipotassium salt BioChemika, for fluorescence
62644	Lucifer Yellow VS dilithium salt BioChemika, for fluorescence, ≥90% (HPLC)
72523	NIR-605-carboxylic acid BioChemika, ~80% (HPCE)
94457	NIR-641 N-succinimidyl ester BioChemika, ≥80% (HPLC)
40741	NIR-641 carboxylic acid BioChemika, ≥90% (HPCE)
12790	NIR-664-N-succinimidyl ester BioChemika, for fluorescence, ≥70% (coupling to amines)
91815	NIR-667 N-succinimidyl ester BioChemika
15167	NIR-797 isothiocyanate BioChemika, for fluorescence, ≥70% (coupling to amines)

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