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RNA-Protein Translation Inhibitors

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Description	Biochem/physiol Actions	Product #
CHAHA ≥98% (HPLC), solid	CHAHA is a novel histone deacetylase (HDAC) inhibitor. CHAHA is as potent as the gold standard SAHA and is more metabolically stable than SAHA or TSA.	C2368
Cordycepin from Cordyceps militaris	Converted to cordycepin 5′-triphosphate. It is incorporated into nucleic acid by poly(A) polymerase, but because it lacks a 3′-hydroxyl group, it causes chain termination. It can be used for 3′-end labeling of RNA.	C3394
Cycloheximide from microbial, ≥94% (TLC)	Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.	C7698
Cycloheximide Ready Made Solution microbial, 100 mg/mL in DMSO, 0.2 μm filtered	Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.	C4859
Diphtheria Toxin from Corynebacterium diphtheriae lyophilized powder	Inhibits protein synthesis by catalyzing ADP-ribosylation of eukaryotic aminoacyltransferase II.	D0564
[Glu⁵²]-Diphtheria toxin from Corynebacterium diphtheriae lyophilized powder		D2189
Emetine dihydrochloride hydrate		E2375
Fusidic acid	Suppresses nitric oxide lysis of pancreatic islet cells. Inhibits protein synthesis in prokaryotes by inhibiting the ribosome-dependent activity of G factor and translocation of peptidyl-tRNA.	F0756
Fusidic acid sodium salt ≥98% (TLC)	Suppresses nitric oxide lysis of pancreatic islet cells. Inhibits protein synthesis in prokaryotes by inhibiting the ribosome-dependent activity of G factor and translocation of peptidyl-tRNA.	F0881
Gentamicin solution 50 mg/mL in deionized water, liquid, sterile-filtered, cell culture tested	Mode of Action: Inhibits protein synthesis by binding to L6 protein of 50S ribosomal subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasma.	G1397
Gentamicin sulfate salt potency: ~600 μg Gentamicin per mg	Mode of action: Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site.^1,² The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface.³ Antimicrobial spectrum: Gram-negative bacteria, Staphylococcus aureus and other Gram-positive bacteria	G3632
Hygromycin B from Streptomyces hygroscopicus powder, cell culture tested, insect cell culture tested	Mode of Action: Blocks polypeptide synthesis and inhibits elongation. For use in the selection and maintenance of prokaryotic and eukaryotic cells.	H3274
ICRF-193	ICRF-193 induces a G2 checkpoint that is associated with an ATR-dependent inhibition of polo-like kinase 1 (plk1) activity and a decrease in cyclin B1 phosphorylation.¹ Induces apoptosis in several cell lines including K562 and Molt-4 cells.^2,^,¹ ICRF-193 is a topoisomerase II inhibitor that targets topoisomerase II-β to a greater extent than it targets topoisomeraseII-α_³ and does not cause DNA damage.³	I4659
Kanamycin sulfate from Streptomyces kanamyceticus Animal Component-free	Mode of Action: Binds to 70S ribosomal subunit; inhibits translocation; elicits miscoding. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasma.	K4000
Kirromycin from Streptomyces collinus ≥90% (HPLC)		K1507
Lycorine hydrochloride 98% (TLC), powder	A selective inhibitor of peptidyl transferase center (PTC).	L5139
Puromycin dihydrochloride Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H₂O, 0.2 μm filtered	Puromycin inhibits the growth of a wide range of eukaryotic and prokaryotic cells by interfering with protein synthesis. It allows the selection of cells expressing the pac gene.	P9620
Puromycin dihydrochloride from Streptomyces alboniger ≥98% (TLC), powder	Nucleoside antibiotic. Protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3'-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells. Acts very quickly and can kill 99% of cells within 2 days, the resistance gene (puromycin acetyltransferase) gives very effective protection.	P7255
Puromycin dihydrochloride from Streptomyces alboniger powder, ≥98% (TLC), cell culture tested	Nucleoside antibiotic. Protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3'-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells. Acts very quickly and can kill 99% of cells within 2 days, the resistance gene (puromycin acetyltransferase) gives very effective protection.	P8833
Saporin from Saponaria officinalis seeds lyophilized powder	A ribosome inactivating protein.	S9896
Spectinomycin Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1, 0.2 μm filtered	Broad spectrum antibiotic produced by the soil bacterium Streptomyces spectabilis. Spectinomycin inhibits protein synthesis (elongation) by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation. Resistance to spectinomycin is conferred by aminoglycoside-3′-adenyltransferase gene (aadA). Spectinomycin is used as a selection marker in plant related transformation systems. Spectinomycin is also used for amplification of low copy number plasmid carrying replicons as Col E1, pMB1 (pBR322 and its derivatives), and p15A/rep (pACYC and its derivatives). The replication of these plasmids relies on long-lived enzymes supplied by the host. Addition of spectinomycin to the plasmid containing cells inhibits replication of the host, while the plasmids continue to replicate for 10-15 hours. The copy number of the plasmid can increase 100-fold, from 20-30 copies to 3000 copies as in the case of ColE1. Mode of Action: Inhibits protein synthesis (elongation) by interfering with peptidyl tRNA translocation. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (Gonnococcus only). Mode of Resistance: Mutation in rpsE (the gene for ribosomal protein S5) prevents binding of spectinomycin.	S0692
Spectinomycin dihydrochloride pentahydrate powder, cell culture tested	Mode of Action: Inhibits protein synthesis (elongation) by interfering with peptidyl tRNA translocation. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (Gonnococcus only). Mode of Resistance: Mutation in rpsE (the gene for ribosomal protein S5) prevents binding of spectinomycin.	S4014
Streptomycin sulfate salt powder	Mode of Action: Inhibits prokaryote protein synthesis. Binds to S12 protein of 30S ribosomal subunit, preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding or inhibiting initiation. Mode of Resistance: Mutation in rpsL (gene for S12 ribosomal protein) prevents binding of streptomycin to ribosome. Aminoglycoside phosphotransferase also inactivates. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.	S6501
Thiostrepton from Streptomyces azureus ≥90% (HPLC)	Peptide antibiotic that prevents the binding of elongation factor G (EF-G) and GTP to the 50S ribosomal subunit.	T8902

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