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Reagents

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Description	Biochem/physiol Actions	Product #
AP-1 Consensus Oligonucleotide		A9590
Cyclo-(Pro-Tyr) >99% (TLC)		C8607
Merbarone ≥98% (HPLC), solid	Selective topoisomerase II inhibitor. Blocks topo II-mediated DNA cleavage without stabilizing DNA-topo II-cleavable complexes. Induces apoptosis in CEM cells via caspase 3 dependent mechanism.	M2070
SIRT1 human recombinant, expressed in Escherichia coli, N-terminal histidine tagged, >90% (SDS-PAGE), buffered aqueous glycerol solution	Sirtuins are a family of NAD⁺ dependent deacetylases that remove an acetyl group from the e-amino group of lysine residues. The proteins within this family are named after the first protein discovered, from yeast, called Sir2 (Silent Information Regulator 2). The proteins are conserved from bacteria to higher eukaryotes. In humans, there are seven Sir2 family members (SIRT1 to SITR7). SIRT1 plays a pivotal role in the regulation of cellular differentiation, metabolism, cell cycle, apoptosis and regulation of p53. Several targets for SIRT1 were identified among them Lys³⁸² of p53.¹ Using RNA interference, additional targets were identified. It was demonstrated that reduced levels of human SIRT1 led to increased acetylation of Histone H4-Lys¹⁶, H4-Lys²⁰, and Histone H3-Lys⁹ as well as histone H1-Lys²⁶.²	S8446
Suptopin-2 ≥98% (HPLC), solid	Suppressor of topoisomerase II inhibition. Reverses cell cycle arrest; bypass of checkpoint function. Has inherent fluorescence and a distinct advantage in identification of molecule targets; effective concentraion in the μM range.	S6321
Topoisomerase I from vaccinia virus buffered aqueous glycerol solution	Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.	T9194
Trichostatin A Ready Made Solution, 5 mM in DMSO (0.2 μm-filtered), from Streptomyces sp.	Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo.¹ Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription.^2,³ TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.⁴	T1952
Trichostatin A ≥98% (HPLC), from Streptomyces sp.	Inhibits histone deacetylase at nanomolar concentrations; resultant histone hyperacetylation leads to chromatin relaxation and modulation of gene expression. May be involved in cell cycle progression of several cell types, inducing cell growth arrest at both G and G/M phases; may induce apoptosis. Enhances the efficacy of anticancer agents that target DNA.	T8552
Verotoxin 2 from Escherichia coli, solution	Verotoxin-2 is a Shiga-like toxin produced by E. coli that epidemiologically is a more important than verotoxin-1 in the development of hemolytic uremia. It is composed of an A subunit that confers its biological activity and a B polypeptide pentamer binds to the globotriaosylceramide (Gb3) receptor inducing its cellular uptake by endocytosis. The A subunit has N-glycosidase activity and cleaves an specific adenine residue in the 28S ribosome thus inhibiting protein synthesis in eukaryotes by blocking the interaction with elongation factors EF-1 and EF-2. Epidemiologically it is more important than verotoxin-1 in the development of hemolytic uremia. The A subunit has N-glycosidase activity, cleaves a specific adenine residue in the 28S ribosome thus inhibiting protein synthesis in eukaryotes by blocking the interaction with elongation factors EF-1 and EF-2. The B polypeptide pentamer binds to the globotriaosylceramide (Gb3) receptor inducing its cellular uptake by endocytosis.	V2513

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