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Phospholipases

Phosphatidic Acid Synthesis
The endoplasmic reticulum (ER) produces most of the lipids required for the generation of new cell membranes. These lipids include cholesterol as well as the phospholipids phosphatidylcholine (PC) and phosphatidic acid (PA). PA is produced by the cleavage of PC to generate PA by the enzyme phospholipase D1 (PLD1). PLD1 is activated by various ligands (agonists) via G-protein-coupled receptors (GPCRs) or receptor tyrosine kinases (RTKs) and is mediated by protein kinase C (PKC) as well as by the small GTPbinding proteins Rho (RhoA, Rac1 and Cdc42) and ADP-ribosylation factor1 (ARF1).
References:
1. Exton, J.H., Phospholipase D. Ann. N.Y. Acad. Sci., 905, 61-68 (2000).
2. McPhail, L.C., et al., A novel protein kinase target for the lipid second messenger phosphatidic acid. Biochim. Biophys. Acta, 1439, 277-290 (1999).
3. Daniel, L.W., et al., Phospholipase D, tumor promoters, proliferation and prostaglandins. Biochim. Biophys. Acta, 1439, 265-276 (1999).

Reset Table

Product #	Description
P3244	Phospholipase A₂ Group GXII murine >85% (SDS-PAGE), recombinant, expressed in Escherichia coli
P7778	Phospholipase A₂ from Naja mossambica mossambica lyophilized powder, ~1,500 units/mg protein
P8913	Phospholipase A₂ from bovine pancreas lyophilized powder, ≥20 units/mg protein
P9279	Phospholipase A₂ from honey bee venom salt-free, lyophilized powder, 600-2400 units/mg protein
P0861	Phospholipase A₂ from porcine pancreas ≥10,000 unit/mL
P6534	Phospholipase A₂ from porcine pancreas ammonium sulfate suspension, ≥600 units/mg protein
P4039	Phospholipase C from Clostridium perfringens (C. welchii) Type XIV, lyophilized powder, ≥150 units/mg protein
P8023	Phospholipase D from Streptomyces chromofuscus lyophilized powder, ≥2,000 units/mg solid
P5542	Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, ≥1,000 units/mg protein (Lowry)
P0640	Phospholipase D from Arachis hypogaea (peanut) Type II, lyophilized powder, 300-700 units/mg protein

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